Strain Fitness in Escherichia coli BW25113 around b3992

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiH and thiG overlap by 4 nucleotidesthiG and thiS are separated by 1 nucleotidesthiS and thiF overlap by 17 nucleotidesthiF and thiE overlap by 8 nucleotidesthiE and thiC overlap by 1 nucleotides b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891 thiH b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658 thiG b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860 thiS b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599 thiF b3993: thiE - thiamine-phosphate pyrophosphorylase (NCBI), at 4,191,592 to 4,192,227 thiE b3994: thiC - thiamine biosynthesis protein ThiC (NCBI), at 4,192,227 to 4,194,122 thiC Position (kb) 4190 4191 4192Strain fitness (log2 ratio) -1 0 1 2at 4190.005 kb on - strand, within thiGat 4190.005 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.253 kb on + strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.287 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.404 kb on + strand, within thiGat 4190.412 kb on - strand, within thiGat 4190.528 kb on - strand, within thiGat 4190.579 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.584 kb on - strandat 4190.586 kb on - strandat 4190.791 kb on + strand, within thiSat 4190.820 kb on + strand, within thiSat 4190.830 kb on + strand, within thiSat 4190.860 kb on - strandat 4190.908 kb on - strandat 4190.968 kb on - strand, within thiFat 4190.968 kb on - strand, within thiFat 4191.030 kb on + strand, within thiFat 4191.121 kb on + strand, within thiFat 4191.121 kb on + strand, within thiFat 4191.129 kb on - strand, within thiFat 4191.234 kb on + strand, within thiFat 4191.236 kb on - strand, within thiFat 4191.248 kb on + strand, within thiFat 4191.269 kb on + strand, within thiFat 4191.282 kb on - strand, within thiFat 4191.310 kb on - strand, within thiFat 4191.368 kb on - strand, within thiFat 4191.376 kb on + strand, within thiFat 4191.468 kb on - strand, within thiFat 4191.475 kb on - strand, within thiFat 4191.475 kb on - strand, within thiFat 4191.483 kb on - strand, within thiFat 4191.483 kb on - strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.489 kb on + strand, within thiFat 4191.680 kb on + strand, within thiEat 4191.690 kb on + strand, within thiEat 4191.731 kb on - strand, within thiEat 4191.755 kb on + strand, within thiEat 4191.755 kb on + strand, within thiEat 4191.859 kb on - strand, within thiEat 4191.869 kb on - strand, within thiEat 4191.938 kb on + strand, within thiEat 4191.983 kb on - strand, within thiEat 4191.995 kb on - strand, within thiEat 4191.995 kb on - strand, within thiEat 4192.021 kb on - strand, within thiEat 4192.021 kb on - strand, within thiEat 4192.054 kb on - strand, within thiEat 4192.054 kb on - strand, within thiEat 4192.122 kb on - strand, within thiEat 4192.122 kb on - strand, within thiEat 4192.151 kb on + strand, within thiEat 4192.151 kb on + strand, within thiEat 4192.156 kb on + strand, within thiEat 4192.158 kb on + strand, within thiEat 4192.165 kb on + strandat 4192.165 kb on + strandat 4192.167 kb on - strandat 4192.167 kb on - strandat 4192.175 kb on + strandat 4192.175 kb on + strandat 4192.176 kb on - strandat 4192.180 kb on + strandat 4192.183 kb on - strandat 4192.183 kb on - strandat 4192.183 kb on - strandat 4192.410 kb on - strandat 4192.474 kb on + strand, within thiCat 4192.474 kb on + strand, within thiCat 4192.547 kb on + strand, within thiCat 4192.576 kb on - strand, within thiCat 4192.576 kb on - strand, within thiC

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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4,190,005 - thiG b3991 0.15 +0.3
4,190,005 - thiG b3991 0.15 +0.5
4,190,187 - thiG b3991 0.39 -0.5
4,190,187 - thiG b3991 0.39 -0.1
4,190,253 + thiG b3991 0.47 -0.1
4,190,267 - thiG b3991 0.49 -0.1
4,190,267 - thiG b3991 0.49 -0.1
4,190,270 + thiG b3991 0.50 -0.8
4,190,270 + thiG b3991 0.50 +0.1
4,190,278 - thiG b3991 0.51 -0.0
4,190,278 - thiG b3991 0.51 +0.0
4,190,287 - thiG b3991 0.52 -0.5
4,190,306 - thiG b3991 0.54 +0.8
4,190,306 - thiG b3991 0.54 -0.3
4,190,404 + thiG b3991 0.67 -1.0
4,190,412 - thiG b3991 0.68 +0.5
4,190,528 - thiG b3991 0.83 +0.3
4,190,579 + thiG b3991 0.90 -0.7
4,190,581 + thiG b3991 0.90 +0.5
4,190,581 + thiG b3991 0.90 -0.3
4,190,584 - +0.3
4,190,586 - -0.1
4,190,791 + thiS b4407 0.65 +0.4
4,190,820 + thiS b4407 0.80 -0.6
4,190,830 + thiS b4407 0.85 -0.1
4,190,860 - -0.3
4,190,908 - -0.9
4,190,968 - thiF b3992 0.16 +0.1
4,190,968 - thiF b3992 0.16 +0.2
4,191,030 + thiF b3992 0.25 +0.2
4,191,121 + thiF b3992 0.37 +0.1
4,191,121 + thiF b3992 0.37 -0.2
4,191,129 - thiF b3992 0.38 -0.6
4,191,234 + thiF b3992 0.52 +0.2
4,191,236 - thiF b3992 0.52 +0.1
4,191,248 + thiF b3992 0.53 +2.4
4,191,269 + thiF b3992 0.56 +0.6
4,191,282 - thiF b3992 0.58 +0.1
4,191,310 - thiF b3992 0.62 +0.4
4,191,368 - thiF b3992 0.69 -0.1
4,191,376 + thiF b3992 0.70 -0.8
4,191,468 - thiF b3992 0.83 -0.2
4,191,475 - thiF b3992 0.83 +0.3
4,191,475 - thiF b3992 0.83 +0.1
4,191,483 - thiF b3992 0.85 -0.6
4,191,483 - thiF b3992 0.85 +0.2
4,191,489 + thiF b3992 0.85 -0.3
4,191,489 + thiF b3992 0.85 +1.9
4,191,489 + thiF b3992 0.85 +0.2
4,191,489 + thiF b3992 0.85 -0.4
4,191,680 + thiE b3993 0.14 -0.3
4,191,690 + thiE b3993 0.15 -1.6
4,191,731 - thiE b3993 0.22 +0.7
4,191,755 + thiE b3993 0.26 -0.3
4,191,755 + thiE b3993 0.26 +0.3
4,191,859 - thiE b3993 0.42 -0.3
4,191,869 - thiE b3993 0.44 +1.0
4,191,938 + thiE b3993 0.54 +0.6
4,191,983 - thiE b3993 0.61 +0.1
4,191,995 - thiE b3993 0.63 +0.1
4,191,995 - thiE b3993 0.63 +0.4
4,192,021 - thiE b3993 0.67 +0.5
4,192,021 - thiE b3993 0.67 +0.7
4,192,054 - thiE b3993 0.73 +0.1
4,192,054 - thiE b3993 0.73 -0.3
4,192,122 - thiE b3993 0.83 -1.7
4,192,122 - thiE b3993 0.83 -0.9
4,192,151 + thiE b3993 0.88 +1.2
4,192,151 + thiE b3993 0.88 -0.2
4,192,156 + thiE b3993 0.89 -1.5
4,192,158 + thiE b3993 0.89 -0.1
4,192,165 + -0.1
4,192,165 + +0.4
4,192,167 - +0.1
4,192,167 - -0.8
4,192,175 + -1.0
4,192,175 + +0.2
4,192,176 - -1.5
4,192,180 + +0.3
4,192,183 - +0.2
4,192,183 - -0.0
4,192,183 - +0.5
4,192,410 - +0.0
4,192,474 + thiC b3994 0.13 +0.2
4,192,474 + thiC b3994 0.13 +0.5
4,192,547 + thiC b3994 0.17 -0.8
4,192,576 - thiC b3994 0.18 +0.3
4,192,576 - thiC b3994 0.18 -0.1

Or see this region's nucleotide sequence