Strain Fitness in Escherichia coli BW25113 around b2722

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycE and hycD are separated by 17 nucleotideshycD and hycC are separated by 2 nucleotides b2721: hycE - hydrogenase 3, large subunit (NCBI), at 2,842,784 to 2,844,493 hycE b2722: hycD - hydrogenase 3, membrane subunit (NCBI), at 2,844,511 to 2,845,434 hycD b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263 hycC Position (kb) 2844 2845 2846Strain fitness (log2 ratio) -2 -1 0 1at 2843.564 kb on + strand, within hycEat 2843.572 kb on - strand, within hycEat 2843.600 kb on + strand, within hycEat 2843.600 kb on + strand, within hycEat 2843.626 kb on + strand, within hycEat 2843.631 kb on + strand, within hycEat 2843.663 kb on + strand, within hycEat 2843.674 kb on - strand, within hycEat 2843.704 kb on + strand, within hycEat 2843.704 kb on + strand, within hycEat 2843.731 kb on - strand, within hycEat 2843.753 kb on - strand, within hycEat 2843.753 kb on - strand, within hycEat 2843.765 kb on + strand, within hycEat 2843.952 kb on + strand, within hycEat 2844.142 kb on + strand, within hycEat 2844.231 kb on + strand, within hycEat 2844.284 kb on - strand, within hycEat 2844.422 kb on + strandat 2844.422 kb on + strandat 2844.443 kb on - strandat 2844.462 kb on + strandat 2844.470 kb on - strandat 2844.470 kb on - strandat 2844.569 kb on - strandat 2844.576 kb on + strandat 2844.607 kb on - strand, within hycDat 2844.761 kb on - strand, within hycDat 2844.789 kb on - strand, within hycDat 2844.937 kb on + strand, within hycDat 2844.945 kb on - strand, within hycDat 2844.945 kb on - strand, within hycDat 2844.984 kb on + strand, within hycDat 2845.140 kb on - strand, within hycDat 2845.212 kb on - strand, within hycDat 2845.231 kb on - strand, within hycDat 2845.269 kb on - strand, within hycDat 2845.320 kb on + strand, within hycDat 2845.428 kb on - strandat 2845.428 kb on - strandat 2845.487 kb on + strandat 2845.594 kb on + strandat 2845.594 kb on + strandat 2846.220 kb on + strand, within hycCat 2846.226 kb on + strand, within hycCat 2846.264 kb on + strand, within hycCat 2846.269 kb on + strand, within hycCat 2846.269 kb on + strand, within hycCat 2846.269 kb on + strand, within hycCat 2846.269 kb on + strand, within hycCat 2846.272 kb on - strand, within hycCat 2846.345 kb on - strand, within hycCat 2846.381 kb on + strand, within hycCat 2846.406 kb on - strand, within hycCat 2846.406 kb on - strand, within hycC

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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2,843,564 + hycE b2721 0.46 +0.6
2,843,572 - hycE b2721 0.46 +0.4
2,843,600 + hycE b2721 0.48 +0.2
2,843,600 + hycE b2721 0.48 -0.1
2,843,626 + hycE b2721 0.49 +0.3
2,843,631 + hycE b2721 0.50 +0.1
2,843,663 + hycE b2721 0.51 -0.1
2,843,674 - hycE b2721 0.52 +0.2
2,843,704 + hycE b2721 0.54 -0.4
2,843,704 + hycE b2721 0.54 -0.3
2,843,731 - hycE b2721 0.55 +0.1
2,843,753 - hycE b2721 0.57 +0.2
2,843,753 - hycE b2721 0.57 +0.4
2,843,765 + hycE b2721 0.57 -0.1
2,843,952 + hycE b2721 0.68 +0.1
2,844,142 + hycE b2721 0.79 +0.6
2,844,231 + hycE b2721 0.85 +0.1
2,844,284 - hycE b2721 0.88 +0.8
2,844,422 + +0.6
2,844,422 + -0.6
2,844,443 - +0.2
2,844,462 + +0.5
2,844,470 - -0.6
2,844,470 - -1.2
2,844,569 - +0.6
2,844,576 + -0.8
2,844,607 - hycD b2722 0.10 +0.0
2,844,761 - hycD b2722 0.27 +0.2
2,844,789 - hycD b2722 0.30 -0.1
2,844,937 + hycD b2722 0.46 -0.0
2,844,945 - hycD b2722 0.47 +0.2
2,844,945 - hycD b2722 0.47 -1.9
2,844,984 + hycD b2722 0.51 +0.0
2,845,140 - hycD b2722 0.68 +0.2
2,845,212 - hycD b2722 0.76 +0.3
2,845,231 - hycD b2722 0.78 -0.5
2,845,269 - hycD b2722 0.82 +1.0
2,845,320 + hycD b2722 0.88 -0.1
2,845,428 - -0.2
2,845,428 - +0.3
2,845,487 + +0.3
2,845,594 + -0.7
2,845,594 + -0.4
2,846,220 + hycC b2723 0.43 +1.1
2,846,226 + hycC b2723 0.43 -0.5
2,846,264 + hycC b2723 0.45 -0.5
2,846,269 + hycC b2723 0.46 +0.7
2,846,269 + hycC b2723 0.46 -0.6
2,846,269 + hycC b2723 0.46 +1.3
2,846,269 + hycC b2723 0.46 +0.8
2,846,272 - hycC b2723 0.46 -0.9
2,846,345 - hycC b2723 0.50 +0.0
2,846,381 + hycC b2723 0.52 -0.3
2,846,406 - hycC b2723 0.53 -0.7
2,846,406 - hycC b2723 0.53 -1.1

Or see this region's nucleotide sequence