Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisJ and argT are separated by 220 nucleotides argT and ubiX are separated by 265 nucleotides ubiX and purF are separated by 94 nucleotides
b2309: hisJ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,424,028 to 2,424,810
hisJ
b2310: argT - lysine/arginine/ornithine transporter subunit (NCBI), at 2,425,031 to 2,425,813
argT
b2311: ubiX - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (NCBI), at 2,426,079 to 2,426,648
ubiX
b2312: purF - amidophosphoribosyltransferase (NCBI), at 2,426,743 to 2,428,260
purF
Position (kb)
2425
2426 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2424.158 kb on - strand, within hisJ at 2424.158 kb on - strand, within hisJ at 2424.158 kb on - strand, within hisJ at 2424.239 kb on - strand, within hisJ at 2424.383 kb on + strand, within hisJ at 2424.384 kb on - strand, within hisJ at 2424.406 kb on - strand, within hisJ at 2424.863 kb on - strand at 2425.153 kb on + strand, within argT at 2425.153 kb on + strand, within argT at 2425.161 kb on - strand, within argT at 2425.161 kb on - strand, within argT at 2425.253 kb on + strand, within argT at 2425.280 kb on + strand, within argT at 2425.286 kb on - strand, within argT at 2425.319 kb on - strand, within argT at 2425.339 kb on - strand, within argT at 2425.353 kb on - strand, within argT at 2425.353 kb on - strand, within argT at 2425.387 kb on - strand, within argT at 2425.387 kb on - strand, within argT at 2425.402 kb on - strand, within argT at 2425.543 kb on + strand, within argT at 2425.702 kb on + strand, within argT at 2425.705 kb on - strand, within argT at 2425.732 kb on - strand, within argT at 2425.742 kb on - strand at 2425.984 kb on - strand at 2426.073 kb on - strand at 2426.795 kb on + strand at 2426.795 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 2,424,158 - hisJ b2309 0.17 +0.2 2,424,158 - hisJ b2309 0.17 +0.1 2,424,158 - hisJ b2309 0.17 -0.1 2,424,239 - hisJ b2309 0.27 +0.1 2,424,383 + hisJ b2309 0.45 +0.8 2,424,384 - hisJ b2309 0.45 +0.4 2,424,406 - hisJ b2309 0.48 +0.0 2,424,863 - -0.3 2,425,153 + argT b2310 0.16 +0.8 2,425,153 + argT b2310 0.16 +0.2 2,425,161 - argT b2310 0.17 -0.8 2,425,161 - argT b2310 0.17 -0.9 2,425,253 + argT b2310 0.28 +0.5 2,425,280 + argT b2310 0.32 +0.4 2,425,286 - argT b2310 0.33 +0.1 2,425,319 - argT b2310 0.37 +0.1 2,425,339 - argT b2310 0.39 +0.7 2,425,353 - argT b2310 0.41 +0.1 2,425,353 - argT b2310 0.41 -0.5 2,425,387 - argT b2310 0.45 +0.7 2,425,387 - argT b2310 0.45 -1.0 2,425,402 - argT b2310 0.47 -0.6 2,425,543 + argT b2310 0.65 -0.1 2,425,702 + argT b2310 0.86 -0.3 2,425,705 - argT b2310 0.86 -0.7 2,425,732 - argT b2310 0.90 -0.2 2,425,742 - -0.5 2,425,984 - +0.1 2,426,073 - +0.2 2,426,795 + -5.4 2,426,795 + -4.3
Or see this region's nucleotide sequence