Strain Fitness in Escherichia coli BW25113 around b2004

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2001 and yeeS overlap by 4 nucleotidesyeeS and yeeT are separated by 62 nucleotidesyeeT and yeeU are separated by 73 nucleotidesyeeU and yeeV are separated by 88 nucleotidesyeeV and yeeW overlap by 4 nucleotides b2001: b2001 - orf, hypothetical protein (VIMSS), at 2,072,803 to 2,074,335 b2001 b2002: yeeS - CP4-44 prophage; predicted DNA repair protein (NCBI), at 2,074,332 to 2,074,778 yeeS b2003: yeeT - CP4-44 prophage; predicted protein (NCBI), at 2,074,841 to 2,075,062 yeeT b2004: yeeU - CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI), at 2,075,136 to 2,075,504 yeeU b2005: yeeV - CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI), at 2,075,593 to 2,075,967 yeeV b2006: yeeW - CP4-44 prophage; predicted protein (RefSeq), at 2,075,964 to 2,076,131 yeeW Position (kb) 2075 2076Strain fitness (log2 ratio) -1 0 1 2at 2074.258 kb on + strandat 2074.258 kb on + strandat 2074.262 kb on + strandat 2074.319 kb on + strandat 2074.396 kb on + strand, within yeeSat 2074.498 kb on + strand, within yeeSat 2074.527 kb on + strand, within yeeSat 2074.527 kb on + strand, within yeeSat 2074.565 kb on - strand, within yeeSat 2074.622 kb on + strand, within yeeSat 2074.649 kb on + strand, within yeeSat 2074.657 kb on - strand, within yeeSat 2074.690 kb on + strand, within yeeSat 2074.696 kb on + strand, within yeeSat 2074.696 kb on - strand, within yeeSat 2074.782 kb on + strandat 2074.865 kb on + strand, within yeeTat 2074.920 kb on - strand, within yeeTat 2074.949 kb on - strand, within yeeTat 2074.949 kb on - strand, within yeeTat 2075.051 kb on + strandat 2075.051 kb on + strandat 2075.051 kb on + strandat 2075.199 kb on - strand, within yeeUat 2075.199 kb on - strand, within yeeUat 2075.201 kb on + strand, within yeeUat 2075.209 kb on + strand, within yeeUat 2075.302 kb on + strand, within yeeUat 2075.407 kb on + strand, within yeeUat 2075.416 kb on + strand, within yeeUat 2075.452 kb on - strand, within yeeUat 2075.452 kb on - strand, within yeeUat 2075.642 kb on + strand, within yeeVat 2075.642 kb on + strand, within yeeVat 2075.827 kb on + strand, within yeeVat 2075.848 kb on - strand, within yeeVat 2075.848 kb on - strand, within yeeVat 2075.933 kb on + strandat 2076.005 kb on + strand, within yeeWat 2076.019 kb on + strand, within yeeWat 2076.019 kb on + strand, within yeeWat 2076.081 kb on + strand, within yeeWat 2076.111 kb on - strand, within yeeWat 2076.111 kb on - strand, within yeeWat 2076.134 kb on + strandat 2076.134 kb on + strandat 2076.161 kb on - strandat 2076.161 kb on - strandat 2076.178 kb on - strandat 2076.184 kb on - strandat 2076.251 kb on + strandat 2076.332 kb on - strandat 2076.415 kb on - strandat 2076.436 kb on + strandat 2076.436 kb on + strandat 2076.462 kb on + strandat 2076.462 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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2,074,258 + +0.6
2,074,258 + -0.3
2,074,262 + -0.9
2,074,319 + +0.1
2,074,396 + yeeS b2002 0.14 -0.0
2,074,498 + yeeS b2002 0.37 -0.2
2,074,527 + yeeS b2002 0.44 +0.8
2,074,527 + yeeS b2002 0.44 +0.1
2,074,565 - yeeS b2002 0.52 +0.2
2,074,622 + yeeS b2002 0.65 -0.8
2,074,649 + yeeS b2002 0.71 +0.1
2,074,657 - yeeS b2002 0.73 -0.4
2,074,690 + yeeS b2002 0.80 +0.6
2,074,696 + yeeS b2002 0.81 +0.1
2,074,696 - yeeS b2002 0.81 -0.0
2,074,782 + -0.1
2,074,865 + yeeT b2003 0.11 +0.0
2,074,920 - yeeT b2003 0.36 -0.0
2,074,949 - yeeT b2003 0.49 -0.3
2,074,949 - yeeT b2003 0.49 -0.1
2,075,051 + +0.7
2,075,051 + -0.1
2,075,051 + -0.1
2,075,199 - yeeU b2004 0.17 -0.1
2,075,199 - yeeU b2004 0.17 +0.1
2,075,201 + yeeU b2004 0.18 +0.6
2,075,209 + yeeU b2004 0.20 -0.2
2,075,302 + yeeU b2004 0.45 +0.4
2,075,407 + yeeU b2004 0.73 -0.7
2,075,416 + yeeU b2004 0.76 +0.1
2,075,452 - yeeU b2004 0.86 +2.0
2,075,452 - yeeU b2004 0.86 +2.2
2,075,642 + yeeV b2005 0.13 +0.3
2,075,642 + yeeV b2005 0.13 -0.3
2,075,827 + yeeV b2005 0.62 +0.3
2,075,848 - yeeV b2005 0.68 +0.2
2,075,848 - yeeV b2005 0.68 -0.0
2,075,933 + +0.5
2,076,005 + yeeW b2006 0.24 +0.2
2,076,019 + yeeW b2006 0.33 +0.1
2,076,019 + yeeW b2006 0.33 -0.5
2,076,081 + yeeW b2006 0.70 -0.2
2,076,111 - yeeW b2006 0.88 -0.3
2,076,111 - yeeW b2006 0.88 +0.9
2,076,134 + +0.4
2,076,134 + -0.2
2,076,161 - +0.3
2,076,161 - -0.5
2,076,178 - +0.1
2,076,184 - +0.4
2,076,251 + -0.1
2,076,332 - -0.9
2,076,415 - -1.4
2,076,436 + -0.0
2,076,436 + -0.1
2,076,462 + +0.3
2,076,462 + +0.2

Or see this region's nucleotide sequence