Strain Fitness in Escherichia coli BW25113 around b1889

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcheA and motB are separated by 4 nucleotidesmotB and motA overlap by 4 nucleotides b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348 cheA b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279 motB b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163 motA Position (kb) 1973 1974 1975Strain fitness (log2 ratio) -1 0 1at 1972.498 kb on + strand, within cheAat 1972.631 kb on + strand, within cheAat 1972.777 kb on + strand, within cheAat 1972.859 kb on + strand, within cheAat 1972.874 kb on + strand, within cheAat 1972.963 kb on + strand, within cheAat 1973.015 kb on - strand, within cheAat 1973.103 kb on - strand, within cheAat 1973.279 kb on - strandat 1973.279 kb on - strandat 1973.443 kb on - strandat 1973.443 kb on - strandat 1973.577 kb on - strand, within motBat 1973.595 kb on - strand, within motBat 1973.697 kb on + strand, within motBat 1973.846 kb on - strand, within motBat 1973.850 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.197 kb on + strandat 1974.226 kb on + strandat 1974.233 kb on + strandat 1974.233 kb on + strandat 1974.414 kb on + strand, within motAat 1974.854 kb on - strand, within motAat 1974.934 kb on + strand, within motAat 1974.934 kb on + strand, within motAat 1975.040 kb on + strand, within motAat 1975.092 kb on + strandat 1975.093 kb on - strandat 1975.173 kb on + strandat 1975.179 kb on + strandat 1975.179 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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1,972,498 + cheA b1888 0.57 +0.2
1,972,631 + cheA b1888 0.63 +0.0
1,972,777 + cheA b1888 0.71 -1.2
1,972,859 + cheA b1888 0.75 +0.2
1,972,874 + cheA b1888 0.76 -0.2
1,972,963 + cheA b1888 0.80 -0.7
1,973,015 - cheA b1888 0.83 +0.2
1,973,103 - cheA b1888 0.87 +0.2
1,973,279 - +0.2
1,973,279 - -0.2
1,973,443 - +0.6
1,973,443 - -0.2
1,973,577 - motB b1889 0.24 +0.7
1,973,595 - motB b1889 0.26 +1.1
1,973,697 + motB b1889 0.37 +0.2
1,973,846 - motB b1889 0.53 +0.5
1,973,850 - motB b1889 0.54 +0.5
1,974,089 - motB b1889 0.79 +0.3
1,974,089 - motB b1889 0.79 +0.9
1,974,197 + +0.6
1,974,226 + +0.5
1,974,233 + -0.1
1,974,233 + +0.7
1,974,414 + motA b1890 0.16 +0.3
1,974,854 - motA b1890 0.65 +1.2
1,974,934 + motA b1890 0.74 +0.5
1,974,934 + motA b1890 0.74 +0.6
1,975,040 + motA b1890 0.86 +0.6
1,975,092 + +0.2
1,975,093 - +0.7
1,975,173 + +0.6
1,975,179 + +0.0
1,975,179 + +0.2

Or see this region's nucleotide sequence