Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflA and pflB are separated by 191 nucleotides pflB and focA are separated by 54 nucleotides
b0902: pflA - pyruvate formate lyase activating enzyme 1 (NCBI), at 949,563 to 950,303
pflA
b0903: pflB - pyruvate formate lyase I (NCBI), at 950,495 to 952,777
pflB
b0904: focA - formate transporter (NCBI), at 952,832 to 953,689
focA
Position (kb) 950
951
952
953 Strain fitness (log2 ratio) -2
-1
0
1
2 at 949.525 kb on - strand at 949.587 kb on - strand at 949.587 kb on - strand at 949.597 kb on - strand at 949.610 kb on - strand at 949.611 kb on + strand at 949.615 kb on - strand at 949.642 kb on - strand, within pflA at 949.642 kb on - strand, within pflA at 949.648 kb on + strand, within pflA at 949.664 kb on - strand, within pflA at 949.675 kb on - strand, within pflA at 949.685 kb on - strand, within pflA at 949.799 kb on - strand, within pflA at 949.860 kb on - strand, within pflA at 949.863 kb on - strand, within pflA at 949.870 kb on - strand, within pflA at 949.997 kb on - strand, within pflA at 949.998 kb on + strand, within pflA at 950.126 kb on - strand, within pflA at 950.126 kb on - strand, within pflA at 950.270 kb on + strand at 950.270 kb on + strand at 950.511 kb on + strand at 950.520 kb on - strand at 950.571 kb on - strand at 950.686 kb on + strand at 950.686 kb on + strand at 950.707 kb on + strand at 950.707 kb on + strand at 950.722 kb on + strand at 950.722 kb on + strand at 950.772 kb on - strand, within pflB at 950.772 kb on - strand, within pflB at 950.777 kb on + strand, within pflB at 950.836 kb on - strand, within pflB at 950.836 kb on - strand, within pflB at 950.854 kb on + strand, within pflB at 950.944 kb on - strand, within pflB at 950.974 kb on - strand, within pflB at 950.974 kb on - strand, within pflB at 950.975 kb on + strand, within pflB at 951.040 kb on + strand, within pflB at 951.040 kb on + strand, within pflB at 951.120 kb on - strand, within pflB at 951.193 kb on - strand, within pflB at 951.269 kb on + strand, within pflB at 951.269 kb on + strand, within pflB at 951.281 kb on + strand, within pflB at 951.288 kb on + strand, within pflB at 951.308 kb on + strand, within pflB at 951.308 kb on - strand, within pflB at 951.374 kb on + strand, within pflB at 951.374 kb on + strand, within pflB at 951.483 kb on - strand, within pflB at 951.533 kb on - strand, within pflB at 951.581 kb on - strand, within pflB at 951.658 kb on - strand, within pflB at 951.658 kb on - strand, within pflB at 951.658 kb on - strand, within pflB at 951.663 kb on - strand, within pflB at 951.690 kb on - strand, within pflB at 951.905 kb on - strand, within pflB at 951.961 kb on - strand, within pflB at 951.963 kb on - strand, within pflB at 951.963 kb on - strand, within pflB at 952.102 kb on - strand, within pflB at 952.107 kb on - strand, within pflB at 952.107 kb on - strand, within pflB at 952.132 kb on + strand, within pflB at 952.170 kb on + strand, within pflB at 952.170 kb on + strand, within pflB at 952.178 kb on - strand, within pflB at 952.178 kb on - strand, within pflB at 952.183 kb on - strand, within pflB at 952.256 kb on + strand, within pflB at 952.256 kb on + strand, within pflB at 952.352 kb on + strand, within pflB at 952.405 kb on - strand, within pflB at 952.444 kb on + strand, within pflB at 952.508 kb on + strand, within pflB at 952.521 kb on - strand, within pflB at 952.527 kb on - strand, within pflB at 952.776 kb on + strand at 952.983 kb on - strand, within focA at 952.983 kb on - strand, within focA at 953.066 kb on - strand, within focA at 953.096 kb on + strand, within focA at 953.155 kb on - strand, within focA at 953.163 kb on - strand, within focA at 953.175 kb on + strand, within focA at 953.175 kb on + strand, within focA at 953.193 kb on + strand, within focA at 953.246 kb on + strand, within focA at 953.423 kb on - strand, within focA at 953.423 kb on - strand, within focA at 953.514 kb on + strand, within focA at 953.516 kb on - strand, within focA at 953.516 kb on - strand, within focA at 953.557 kb on + strand, within focA at 953.566 kb on - strand, within focA at 953.581 kb on + strand, within focA at 953.610 kb on + strand at 953.611 kb on + strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.618 kb on - strand at 953.659 kb on + strand at 953.682 kb on + strand at 953.682 kb on + strand at 953.691 kb on - strand at 953.697 kb on - strand at 953.774 kb on + strand at 953.774 kb on + strand at 953.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 949,525 - -0.0 949,587 - +0.2 949,587 - +0.4 949,597 - +0.4 949,610 - +0.4 949,611 + +2.2 949,615 - +1.2 949,642 - pflA b0902 0.11 -0.3 949,642 - pflA b0902 0.11 -0.6 949,648 + pflA b0902 0.11 -0.6 949,664 - pflA b0902 0.14 -0.1 949,675 - pflA b0902 0.15 +0.4 949,685 - pflA b0902 0.16 -0.6 949,799 - pflA b0902 0.32 -0.3 949,860 - pflA b0902 0.40 -0.0 949,863 - pflA b0902 0.40 -1.0 949,870 - pflA b0902 0.41 -0.4 949,997 - pflA b0902 0.59 -0.1 949,998 + pflA b0902 0.59 -0.5 950,126 - pflA b0902 0.76 +0.5 950,126 - pflA b0902 0.76 +0.4 950,270 + -0.8 950,270 + +0.1 950,511 + -1.3 950,520 - +0.3 950,571 - +2.5 950,686 + -0.5 950,686 + +0.8 950,707 + +0.1 950,707 + +0.3 950,722 + -1.5 950,722 + -0.4 950,772 - pflB b0903 0.12 +0.2 950,772 - pflB b0903 0.12 -0.2 950,777 + pflB b0903 0.12 -0.2 950,836 - pflB b0903 0.15 -1.0 950,836 - pflB b0903 0.15 -0.2 950,854 + pflB b0903 0.16 -0.9 950,944 - pflB b0903 0.20 +0.3 950,974 - pflB b0903 0.21 -0.4 950,974 - pflB b0903 0.21 -1.1 950,975 + pflB b0903 0.21 +0.2 951,040 + pflB b0903 0.24 +0.5 951,040 + pflB b0903 0.24 +0.7 951,120 - pflB b0903 0.27 +0.0 951,193 - pflB b0903 0.31 +0.6 951,269 + pflB b0903 0.34 +0.6 951,269 + pflB b0903 0.34 -0.4 951,281 + pflB b0903 0.34 -0.6 951,288 + pflB b0903 0.35 -0.3 951,308 + pflB b0903 0.36 +0.0 951,308 - pflB b0903 0.36 -0.1 951,374 + pflB b0903 0.39 +0.6 951,374 + pflB b0903 0.39 +0.6 951,483 - pflB b0903 0.43 -0.3 951,533 - pflB b0903 0.45 +0.3 951,581 - pflB b0903 0.48 +0.3 951,658 - pflB b0903 0.51 +0.1 951,658 - pflB b0903 0.51 +0.2 951,658 - pflB b0903 0.51 -0.1 951,663 - pflB b0903 0.51 -0.1 951,690 - pflB b0903 0.52 +0.8 951,905 - pflB b0903 0.62 -0.2 951,961 - pflB b0903 0.64 +0.1 951,963 - pflB b0903 0.64 -1.9 951,963 - pflB b0903 0.64 -2.0 952,102 - pflB b0903 0.70 -0.2 952,107 - pflB b0903 0.71 -0.1 952,107 - pflB b0903 0.71 -0.7 952,132 + pflB b0903 0.72 -0.5 952,170 + pflB b0903 0.73 +0.1 952,170 + pflB b0903 0.73 -0.2 952,178 - pflB b0903 0.74 +0.2 952,178 - pflB b0903 0.74 -0.0 952,183 - pflB b0903 0.74 -1.3 952,256 + pflB b0903 0.77 -0.3 952,256 + pflB b0903 0.77 +0.0 952,352 + pflB b0903 0.81 +0.8 952,405 - pflB b0903 0.84 +0.2 952,444 + pflB b0903 0.85 +0.3 952,508 + pflB b0903 0.88 -0.7 952,521 - pflB b0903 0.89 -0.5 952,527 - pflB b0903 0.89 +0.3 952,776 + -1.0 952,983 - focA b0904 0.18 -1.0 952,983 - focA b0904 0.18 +0.1 953,066 - focA b0904 0.27 -0.3 953,096 + focA b0904 0.31 +0.1 953,155 - focA b0904 0.38 -0.3 953,163 - focA b0904 0.39 -0.3 953,175 + focA b0904 0.40 +0.2 953,175 + focA b0904 0.40 +0.4 953,193 + focA b0904 0.42 -0.0 953,246 + focA b0904 0.48 +0.7 953,423 - focA b0904 0.69 -0.2 953,423 - focA b0904 0.69 -0.0 953,514 + focA b0904 0.79 +0.7 953,516 - focA b0904 0.80 -0.7 953,516 - focA b0904 0.80 -1.0 953,557 + focA b0904 0.84 +1.1 953,566 - focA b0904 0.86 -0.2 953,581 + focA b0904 0.87 +0.6 953,610 + +0.3 953,611 + -0.5 953,618 - -0.6 953,618 - -1.2 953,618 - +0.5 953,618 - +1.8 953,618 - +0.2 953,618 - -0.9 953,618 - -0.1 953,618 - -0.9 953,618 - +1.4 953,659 + +0.3 953,682 + +0.6 953,682 + -1.0 953,691 - +1.2 953,697 - -0.8 953,774 + +0.1 953,774 + -0.4 953,774 + +0.4
Or see this region's nucleotide sequence