Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjC and nfsA overlap by 17 nucleotides nfsA and rimK are separated by 60 nucleotides rimK and ybjN are separated by 87 nucleotides ybjN and potF are separated by 350 nucleotides
b0850: ybjC - predicted inner membrane protein (NCBI), at 890,136 to 890,423
ybjC
b0851: nfsA - nitroreductase A, NADPH-dependent, FMN-dependent (NCBI), at 890,407 to 891,129
nfsA
b0852: rimK - ribosomal protein S6 modification protein (NCBI), at 891,190 to 892,092
rimK
b0853: ybjN - predicted oxidoreductase (NCBI), at 892,180 to 892,656
ybjN
b0854: potF - putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 893,007 to 894,119
potF
Position (kb) 891
892
893 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 890.213 kb on + strand, within ybjC at 890.218 kb on + strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.326 kb on + strand, within ybjC at 890.491 kb on - strand, within nfsA at 890.714 kb on - strand, within nfsA at 890.715 kb on - strand, within nfsA at 890.783 kb on + strand, within nfsA at 890.792 kb on - strand, within nfsA at 890.958 kb on + strand, within nfsA at 890.979 kb on + strand, within nfsA at 890.979 kb on + strand, within nfsA at 891.034 kb on + strand, within nfsA at 891.057 kb on - strand at 891.213 kb on + strand at 891.222 kb on + strand at 891.243 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.251 kb on + strand at 891.254 kb on + strand at 891.387 kb on - strand, within rimK at 891.387 kb on - strand, within rimK at 891.703 kb on - strand, within rimK at 891.703 kb on - strand, within rimK at 891.766 kb on - strand, within rimK at 891.868 kb on - strand, within rimK at 892.020 kb on - strand at 892.167 kb on + strand at 892.206 kb on + strand at 892.244 kb on - strand, within ybjN at 892.284 kb on - strand, within ybjN at 892.284 kb on - strand, within ybjN at 892.304 kb on + strand, within ybjN at 892.389 kb on - strand, within ybjN at 892.389 kb on - strand, within ybjN at 892.411 kb on + strand, within ybjN at 892.411 kb on + strand, within ybjN at 892.524 kb on + strand, within ybjN at 892.550 kb on - strand, within ybjN at 892.609 kb on - strand at 892.609 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 890,213 + ybjC b0850 0.27 +1.0 890,218 + ybjC b0850 0.28 +0.5 890,232 - ybjC b0850 0.33 +0.6 890,232 - ybjC b0850 0.33 -0.0 890,326 + ybjC b0850 0.66 +1.3 890,491 - nfsA b0851 0.12 +2.9 890,714 - nfsA b0851 0.42 +0.0 890,715 - nfsA b0851 0.43 -0.4 890,783 + nfsA b0851 0.52 -0.5 890,792 - nfsA b0851 0.53 +1.0 890,958 + nfsA b0851 0.76 +0.0 890,979 + nfsA b0851 0.79 -0.6 890,979 + nfsA b0851 0.79 -0.6 891,034 + nfsA b0851 0.87 -0.4 891,057 - +0.2 891,213 + +1.0 891,222 + +0.9 891,243 + +0.1 891,251 + +0.1 891,251 + -0.7 891,251 + -0.3 891,251 + -0.6 891,254 + +0.6 891,387 - rimK b0852 0.22 +0.2 891,387 - rimK b0852 0.22 +0.8 891,703 - rimK b0852 0.57 +0.5 891,703 - rimK b0852 0.57 +1.0 891,766 - rimK b0852 0.64 -0.1 891,868 - rimK b0852 0.75 +0.0 892,020 - +0.4 892,167 + -0.1 892,206 + -0.8 892,244 - ybjN b0853 0.13 +1.0 892,284 - ybjN b0853 0.22 +0.9 892,284 - ybjN b0853 0.22 -0.5 892,304 + ybjN b0853 0.26 +0.1 892,389 - ybjN b0853 0.44 +0.2 892,389 - ybjN b0853 0.44 -0.4 892,411 + ybjN b0853 0.48 -1.4 892,411 + ybjN b0853 0.48 -0.9 892,524 + ybjN b0853 0.72 -0.7 892,550 - ybjN b0853 0.78 -2.1 892,609 - -1.1 892,609 - +1.6
Or see this region's nucleotide sequence