Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiV and ybiW are separated by 145 nucleotides ybiW and ybiY are separated by 5 nucleotides
b0822: ybiV - predicted hydrolase (NCBI), at 858,436 to 859,251
ybiV
b0823: ybiW - predicted pyruvate formate lyase (NCBI), at 859,397 to 861,829
ybiW
b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734
ybiY
Position (kb) 859
860
861
862 Strain fitness (log2 ratio) -1
0
1
2 at 858.400 kb on - strand at 858.400 kb on - strand at 858.424 kb on + strand at 858.439 kb on + strand at 858.457 kb on + strand at 858.460 kb on + strand at 858.556 kb on - strand, within ybiV at 858.604 kb on - strand, within ybiV at 858.759 kb on + strand, within ybiV at 858.796 kb on - strand, within ybiV at 858.920 kb on - strand, within ybiV at 859.134 kb on + strand, within ybiV at 859.134 kb on + strand, within ybiV at 859.446 kb on - strand at 859.446 kb on - strand at 859.472 kb on + strand at 859.486 kb on + strand at 859.569 kb on + strand at 859.569 kb on + strand at 859.651 kb on + strand, within ybiW at 859.661 kb on - strand, within ybiW at 859.740 kb on + strand, within ybiW at 859.775 kb on + strand, within ybiW at 859.783 kb on - strand, within ybiW at 859.783 kb on - strand, within ybiW at 859.876 kb on + strand, within ybiW at 859.884 kb on - strand, within ybiW at 859.884 kb on - strand, within ybiW at 859.967 kb on - strand, within ybiW at 860.047 kb on + strand, within ybiW at 860.081 kb on - strand, within ybiW at 860.081 kb on - strand, within ybiW at 860.146 kb on + strand, within ybiW at 860.210 kb on + strand, within ybiW at 860.228 kb on + strand, within ybiW at 860.228 kb on + strand, within ybiW at 860.245 kb on - strand, within ybiW at 860.295 kb on + strand, within ybiW at 860.295 kb on + strand, within ybiW at 860.306 kb on + strand, within ybiW at 860.307 kb on - strand, within ybiW at 860.310 kb on + strand, within ybiW at 860.469 kb on - strand, within ybiW at 860.510 kb on + strand, within ybiW at 860.623 kb on + strand, within ybiW at 860.631 kb on - strand, within ybiW at 860.634 kb on - strand, within ybiW at 860.687 kb on - strand, within ybiW at 860.764 kb on + strand, within ybiW at 860.884 kb on - strand, within ybiW at 861.029 kb on - strand, within ybiW at 861.287 kb on - strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.715 kb on + strand at 861.715 kb on + strand at 861.723 kb on - strand at 861.728 kb on - strand at 861.749 kb on - strand at 861.947 kb on - strand, within ybiY at 862.001 kb on + strand, within ybiY at 862.063 kb on - strand, within ybiY at 862.219 kb on - strand, within ybiY at 862.295 kb on + strand, within ybiY at 862.398 kb on - strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.439 kb on - strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.462 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.580 kb on + strand, within ybiY at 862.600 kb on + strand, within ybiY at 862.697 kb on - strand at 862.717 kb on - strand at 862.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 858,400 - +0.3 858,400 - +0.5 858,424 + -0.7 858,439 + -0.1 858,457 + -0.4 858,460 + +0.4 858,556 - ybiV b0822 0.15 +0.3 858,604 - ybiV b0822 0.21 +0.4 858,759 + ybiV b0822 0.40 +0.6 858,796 - ybiV b0822 0.44 +0.1 858,920 - ybiV b0822 0.59 +0.6 859,134 + ybiV b0822 0.86 -0.1 859,134 + ybiV b0822 0.86 -0.8 859,446 - +0.4 859,446 - -0.2 859,472 + -0.2 859,486 + -1.2 859,569 + +0.6 859,569 + +0.4 859,651 + ybiW b0823 0.10 -0.5 859,661 - ybiW b0823 0.11 +0.1 859,740 + ybiW b0823 0.14 +0.4 859,775 + ybiW b0823 0.16 -0.8 859,783 - ybiW b0823 0.16 -0.0 859,783 - ybiW b0823 0.16 +0.5 859,876 + ybiW b0823 0.20 -0.0 859,884 - ybiW b0823 0.20 +0.2 859,884 - ybiW b0823 0.20 -1.6 859,967 - ybiW b0823 0.23 +0.6 860,047 + ybiW b0823 0.27 -0.0 860,081 - ybiW b0823 0.28 -0.2 860,081 - ybiW b0823 0.28 -0.4 860,146 + ybiW b0823 0.31 -0.1 860,210 + ybiW b0823 0.33 +0.3 860,228 + ybiW b0823 0.34 +0.8 860,228 + ybiW b0823 0.34 +1.9 860,245 - ybiW b0823 0.35 +0.3 860,295 + ybiW b0823 0.37 +0.8 860,295 + ybiW b0823 0.37 +0.1 860,306 + ybiW b0823 0.37 +0.7 860,307 - ybiW b0823 0.37 -0.6 860,310 + ybiW b0823 0.38 +0.4 860,469 - ybiW b0823 0.44 +0.0 860,510 + ybiW b0823 0.46 +0.1 860,623 + ybiW b0823 0.50 -0.8 860,631 - ybiW b0823 0.51 +0.2 860,634 - ybiW b0823 0.51 -0.5 860,687 - ybiW b0823 0.53 +0.1 860,764 + ybiW b0823 0.56 +0.0 860,884 - ybiW b0823 0.61 -1.0 861,029 - ybiW b0823 0.67 -0.1 861,287 - ybiW b0823 0.78 -0.1 861,428 + ybiW b0823 0.83 -0.5 861,428 + ybiW b0823 0.83 -1.1 861,428 + ybiW b0823 0.83 -0.3 861,715 + -0.7 861,715 + -0.6 861,723 - -0.1 861,728 - +0.2 861,749 - +0.4 861,947 - ybiY b0824 0.12 +0.3 862,001 + ybiY b0824 0.18 +0.4 862,063 - ybiY b0824 0.25 +0.7 862,219 - ybiY b0824 0.43 +0.2 862,295 + ybiY b0824 0.51 +0.5 862,398 - ybiY b0824 0.63 -0.6 862,435 + ybiY b0824 0.67 -0.6 862,435 + ybiY b0824 0.67 +0.3 862,439 - ybiY b0824 0.67 -0.4 862,442 + ybiY b0824 0.67 -0.5 862,442 + ybiY b0824 0.67 -0.2 862,462 + ybiY b0824 0.70 -0.0 862,575 + ybiY b0824 0.82 +0.1 862,575 + ybiY b0824 0.82 +0.1 862,580 + ybiY b0824 0.83 -0.8 862,600 + ybiY b0824 0.85 -0.9 862,697 - -0.9 862,717 - -1.1 862,828 + -1.1
Or see this region's nucleotide sequence