Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entF and fepE are separated by 215 nucleotides fepE and fepC overlap by 4 nucleotides fepC and fepG overlap by 4 nucleotides
b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261
entF
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422
fepC
b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411
fepG
Position (kb) 617
618
619 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 616.529 kb on + strand, within entF at 616.529 kb on + strand, within entF at 616.645 kb on + strand, within entF at 616.779 kb on - strand, within entF at 616.779 kb on - strand, within entF at 616.793 kb on - strand, within entF at 616.814 kb on + strand, within entF at 616.868 kb on + strand, within entF at 616.988 kb on - strand at 616.993 kb on - strand at 617.023 kb on - strand at 617.024 kb on + strand at 617.024 kb on + strand at 617.038 kb on - strand at 617.048 kb on + strand at 617.066 kb on - strand at 617.120 kb on + strand at 617.121 kb on + strand at 617.122 kb on + strand at 617.133 kb on + strand at 617.135 kb on - strand at 617.136 kb on - strand at 617.136 kb on - strand at 617.138 kb on + strand at 617.146 kb on - strand at 617.155 kb on + strand at 617.160 kb on - strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.166 kb on + strand at 617.168 kb on + strand at 617.168 kb on - strand at 617.170 kb on + strand at 617.175 kb on + strand at 617.175 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.182 kb on + strand at 617.183 kb on - strand at 617.195 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.235 kb on + strand at 617.247 kb on + strand at 617.272 kb on + strand at 617.587 kb on - strand at 617.616 kb on + strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.701 kb on - strand, within fepE at 617.755 kb on - strand, within fepE at 617.849 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.947 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 618.013 kb on + strand, within fepE at 618.013 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.029 kb on - strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.053 kb on - strand, within fepE at 618.071 kb on + strand, within fepE at 618.082 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.114 kb on + strand, within fepE at 618.120 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.190 kb on + strand, within fepE at 618.250 kb on - strand, within fepE at 618.267 kb on - strand, within fepE at 618.271 kb on - strand, within fepE at 618.300 kb on - strand, within fepE at 618.305 kb on - strand, within fepE at 618.361 kb on - strand, within fepE at 618.371 kb on - strand, within fepE at 618.377 kb on + strand, within fepE at 618.377 kb on + strand, within fepE at 618.383 kb on - strand, within fepE at 618.386 kb on - strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.418 kb on - strand, within fepE at 618.457 kb on + strand, within fepE at 618.484 kb on + strand, within fepE at 618.498 kb on + strand at 618.500 kb on - strand at 618.500 kb on - strand at 618.520 kb on - strand at 618.545 kb on - strand at 618.561 kb on - strand at 618.561 kb on - strand at 618.598 kb on - strand at 618.721 kb on + strand, within fepC at 618.725 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.876 kb on - strand, within fepC at 618.905 kb on - strand, within fepC at 618.927 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.970 kb on - strand, within fepC at 619.003 kb on + strand, within fepC at 619.038 kb on + strand, within fepC at 619.066 kb on - strand, within fepC at 619.131 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.261 kb on + strand, within fepC at 619.375 kb on + strand at 619.568 kb on - strand, within fepG at 619.568 kb on - strand, within fepG
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 616,529 + entF b0586 0.81 -0.5 616,529 + entF b0586 0.81 -0.7 616,645 + entF b0586 0.84 +0.2 616,779 - entF b0586 0.88 -0.6 616,779 - entF b0586 0.88 -0.2 616,793 - entF b0586 0.88 +0.1 616,814 + entF b0586 0.88 -1.1 616,868 + entF b0586 0.90 -0.2 616,988 - -0.6 616,993 - +0.3 617,023 - -0.4 617,024 + -0.0 617,024 + -0.1 617,038 - -0.3 617,048 + +0.3 617,066 - +0.1 617,120 + -0.4 617,121 + -1.0 617,122 + +1.6 617,133 + -0.5 617,135 - -1.0 617,136 - -1.0 617,136 - -1.2 617,138 + +0.2 617,146 - -0.5 617,155 + -0.4 617,160 - +0.7 617,162 + -1.0 617,162 + -0.9 617,162 + -0.1 617,166 + -0.2 617,168 + -0.7 617,168 - +0.4 617,170 + -0.2 617,175 + -0.0 617,175 + +0.3 617,181 + -1.2 617,181 + -0.7 617,182 + -0.8 617,183 - +0.3 617,195 + +0.1 617,206 + -0.4 617,206 + -0.5 617,206 + +0.2 617,206 + -0.5 617,206 + -1.6 617,206 + -1.1 617,235 + +0.2 617,247 + -0.7 617,272 + -1.2 617,587 - -0.9 617,616 + fepE b0587 0.12 +2.3 617,644 - fepE b0587 0.15 -0.9 617,644 - fepE b0587 0.15 -0.2 617,644 - fepE b0587 0.15 +1.0 617,701 - fepE b0587 0.20 -0.5 617,755 - fepE b0587 0.25 -0.3 617,849 + fepE b0587 0.33 -1.0 617,893 + fepE b0587 0.37 -0.8 617,893 + fepE b0587 0.37 -0.6 617,947 - fepE b0587 0.41 +3.3 617,985 - fepE b0587 0.45 +1.3 617,985 - fepE b0587 0.45 +0.7 617,986 - fepE b0587 0.45 +0.2 617,986 - fepE b0587 0.45 +2.3 617,986 - fepE b0587 0.45 +2.3 618,013 + fepE b0587 0.47 -0.4 618,013 + fepE b0587 0.47 +1.1 618,018 + fepE b0587 0.48 +1.3 618,018 + fepE b0587 0.48 +0.7 618,029 - fepE b0587 0.49 +0.3 618,035 + fepE b0587 0.49 +1.0 618,035 + fepE b0587 0.49 +1.1 618,035 + fepE b0587 0.49 +0.7 618,045 + fepE b0587 0.50 -1.2 618,045 + fepE b0587 0.50 +1.1 618,053 - fepE b0587 0.51 +2.3 618,071 + fepE b0587 0.52 -1.0 618,082 + fepE b0587 0.53 -0.6 618,104 + fepE b0587 0.55 -0.9 618,104 + fepE b0587 0.55 +1.3 618,114 + fepE b0587 0.56 +1.3 618,120 - fepE b0587 0.57 +2.9 618,151 - fepE b0587 0.59 -0.0 618,151 - fepE b0587 0.59 +2.3 618,190 + fepE b0587 0.63 -0.3 618,250 - fepE b0587 0.68 -1.7 618,267 - fepE b0587 0.70 -0.3 618,271 - fepE b0587 0.70 +0.1 618,300 - fepE b0587 0.73 -0.2 618,305 - fepE b0587 0.73 -1.0 618,361 - fepE b0587 0.78 +0.8 618,371 - fepE b0587 0.79 +0.0 618,377 + fepE b0587 0.79 -0.4 618,377 + fepE b0587 0.79 -0.7 618,383 - fepE b0587 0.80 +0.2 618,386 - fepE b0587 0.80 +0.1 618,412 + fepE b0587 0.82 -0.1 618,412 + fepE b0587 0.82 +0.2 618,412 + fepE b0587 0.82 +0.3 618,418 - fepE b0587 0.83 -0.5 618,457 + fepE b0587 0.86 -0.9 618,484 + fepE b0587 0.89 -0.0 618,498 + +0.2 618,500 - +0.3 618,500 - +0.5 618,520 - -0.4 618,545 - +0.2 618,561 - +0.7 618,561 - +0.4 618,598 - +0.0 618,721 + fepC b0588 0.14 -3.0 618,725 - fepC b0588 0.14 -5.0 618,815 - fepC b0588 0.25 -2.3 618,815 - fepC b0588 0.25 -5.6 618,876 - fepC b0588 0.33 -4.0 618,905 - fepC b0588 0.37 -4.9 618,927 - fepC b0588 0.39 -4.9 618,935 - fepC b0588 0.40 -2.6 618,935 - fepC b0588 0.40 -3.5 618,970 - fepC b0588 0.44 -2.6 619,003 + fepC b0588 0.49 -2.9 619,038 + fepC b0588 0.53 -3.7 619,066 - fepC b0588 0.56 -3.4 619,131 - fepC b0588 0.64 -3.2 619,259 - fepC b0588 0.80 -3.9 619,259 - fepC b0588 0.80 -2.9 619,261 + fepC b0588 0.80 -4.8 619,375 + -4.8 619,568 - fepG b0589 0.15 -3.3 619,568 - fepG b0589 0.15 -1.7
Or see this region's nucleotide sequence