Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispA and xseB overlap by 1 nucleotides xseB and thiI are separated by 205 nucleotides thiI and thiJ are separated by 53 nucleotides thiJ and panE overlap by 38 nucleotides
b0421: ispA - geranyltranstransferase (NCBI), at 439,426 to 440,325
ispA
b0422: xseB - exodeoxyribonuclease VII small subunit (NCBI), at 440,325 to 440,567
xseB
b0423: thiI - thiamine biosynthesis protein ThiI (NCBI), at 440,773 to 442,221
thiI
b0424: thiJ - 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS), at 442,275 to 442,865
thiJ
b0425: panE - 2-dehydropantoate 2-reductase (NCBI), at 442,828 to 443,739
panE
Position (kb) 440
441
442
443 Strain fitness (log2 ratio) -2
-1
0
1 at 440.335 kb on - strand at 440.546 kb on - strand at 440.581 kb on - strand at 440.581 kb on - strand at 440.609 kb on + strand at 440.650 kb on + strand at 440.650 kb on + strand at 440.650 kb on - strand at 440.716 kb on - strand at 440.750 kb on - strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.890 kb on - strand at 440.890 kb on - strand at 440.968 kb on + strand, within thiI at 440.968 kb on + strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.040 kb on - strand, within thiI at 441.066 kb on - strand, within thiI at 441.100 kb on + strand, within thiI at 441.430 kb on - strand, within thiI at 441.471 kb on + strand, within thiI at 441.575 kb on - strand, within thiI at 441.575 kb on - strand, within thiI at 441.603 kb on + strand, within thiI at 441.605 kb on - strand, within thiI at 441.711 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 441.723 kb on - strand, within thiI at 442.032 kb on + strand, within thiI at 442.079 kb on + strand at 442.111 kb on + strand at 442.111 kb on + strand at 442.126 kb on + strand at 442.169 kb on + strand at 442.169 kb on + strand at 442.173 kb on + strand at 442.173 kb on + strand at 442.175 kb on + strand at 442.183 kb on + strand at 442.189 kb on + strand at 442.189 kb on + strand at 442.191 kb on - strand at 442.197 kb on - strand at 442.197 kb on - strand at 442.207 kb on - strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.267 kb on + strand at 442.345 kb on - strand, within thiJ at 442.385 kb on + strand, within thiJ at 442.387 kb on + strand, within thiJ at 442.425 kb on + strand, within thiJ at 442.438 kb on + strand, within thiJ at 442.514 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.576 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.660 kb on - strand, within thiJ at 442.668 kb on + strand, within thiJ at 442.684 kb on + strand, within thiJ at 442.717 kb on - strand, within thiJ at 442.796 kb on - strand, within thiJ at 442.814 kb on + strand at 442.823 kb on + strand at 442.823 kb on + strand at 442.831 kb on - strand at 442.848 kb on - strand at 442.848 kb on - strand at 442.853 kb on - strand at 442.902 kb on - strand at 442.917 kb on - strand at 443.001 kb on - strand, within panE at 443.091 kb on - strand, within panE at 443.139 kb on + strand, within panE at 443.147 kb on - strand, within panE at 443.147 kb on - strand, within panE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 440,335 - +0.3 440,546 - +0.5 440,581 - -1.2 440,581 - -0.5 440,609 + +1.1 440,650 + +0.9 440,650 + +0.3 440,650 - -0.6 440,716 - +0.1 440,750 - -0.9 440,882 + -0.8 440,882 + +0.4 440,882 + -0.6 440,890 - +0.2 440,890 - +0.7 440,968 + thiI b0423 0.13 +0.4 440,968 + thiI b0423 0.13 +0.2 440,976 - thiI b0423 0.14 +0.2 440,976 - thiI b0423 0.14 +1.2 440,976 - thiI b0423 0.14 +0.4 441,031 - thiI b0423 0.18 -0.2 441,031 - thiI b0423 0.18 -0.7 441,040 - thiI b0423 0.18 -0.4 441,066 - thiI b0423 0.20 +0.2 441,100 + thiI b0423 0.23 +0.1 441,430 - thiI b0423 0.45 +0.7 441,471 + thiI b0423 0.48 -0.1 441,575 - thiI b0423 0.55 +1.1 441,575 - thiI b0423 0.55 +0.1 441,603 + thiI b0423 0.57 +0.5 441,605 - thiI b0423 0.57 -0.5 441,711 - thiI b0423 0.65 -0.2 441,723 - thiI b0423 0.66 -0.2 441,723 - thiI b0423 0.66 +1.6 442,032 + thiI b0423 0.87 +0.5 442,079 + +0.2 442,111 + -0.1 442,111 + +0.3 442,126 + -0.1 442,169 + +0.4 442,169 + +0.7 442,173 + +1.0 442,173 + +0.3 442,175 + +1.0 442,183 + +0.3 442,189 + +0.2 442,189 + +0.6 442,191 - -0.2 442,197 - +0.7 442,197 - -0.1 442,207 - +0.3 442,267 + -0.1 442,267 + -0.2 442,267 + +0.3 442,345 - thiJ b0424 0.12 -0.4 442,385 + thiJ b0424 0.19 +0.0 442,387 + thiJ b0424 0.19 -0.4 442,425 + thiJ b0424 0.25 +0.2 442,438 + thiJ b0424 0.28 +0.0 442,514 - thiJ b0424 0.40 +0.2 442,576 - thiJ b0424 0.51 -0.1 442,576 - thiJ b0424 0.51 +0.4 442,660 - thiJ b0424 0.65 +1.0 442,660 - thiJ b0424 0.65 -2.1 442,668 + thiJ b0424 0.66 -0.1 442,684 + thiJ b0424 0.69 -0.8 442,717 - thiJ b0424 0.75 +0.0 442,796 - thiJ b0424 0.88 -0.0 442,814 + -0.1 442,823 + -0.1 442,823 + +0.3 442,831 - +0.3 442,848 - +0.5 442,848 - -0.3 442,853 - -0.3 442,902 - +0.5 442,917 - -0.3 443,001 - panE b0425 0.19 -0.6 443,091 - panE b0425 0.29 +0.2 443,139 + panE b0425 0.34 +0.1 443,147 - panE b0425 0.35 +0.2 443,147 - panE b0425 0.35 -0.3
Or see this region's nucleotide sequence