Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddlA and yaiB are separated by 462 nucleotides yaiB and phoA are separated by 100 nucleotides
b0381: ddlA - D-alanylalanine synthetase (NCBI), at 399,053 to 400,147
ddlA
b0382: yaiB - hypothetical protein (NCBI), at 400,610 to 400,870
yaiB
b0383: phoA - alkaline phosphatase (VIMSS), at 400,971 to 402,386
phoA
Position (kb) 400
401 Strain fitness (log2 ratio) -1
0
1
2
3 at 399.659 kb on - strand, within ddlA at 399.707 kb on - strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.787 kb on + strand, within ddlA at 399.824 kb on + strand, within ddlA at 399.916 kb on + strand, within ddlA at 399.933 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA at 400.092 kb on + strand at 400.092 kb on + strand at 400.093 kb on + strand at 400.093 kb on + strand at 400.168 kb on + strand at 400.178 kb on - strand at 400.240 kb on + strand at 400.240 kb on + strand at 400.251 kb on + strand at 400.258 kb on + strand at 400.263 kb on + strand at 400.264 kb on + strand at 400.282 kb on - strand at 400.288 kb on - strand at 400.373 kb on + strand at 400.559 kb on - strand at 400.561 kb on + strand at 400.638 kb on - strand, within yaiB at 400.667 kb on + strand, within yaiB at 400.675 kb on + strand, within yaiB at 400.684 kb on + strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.692 kb on - strand, within yaiB at 400.707 kb on + strand, within yaiB at 400.715 kb on - strand, within yaiB at 400.750 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.762 kb on + strand, within yaiB at 400.770 kb on - strand, within yaiB at 400.770 kb on - strand, within yaiB at 400.775 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.777 kb on + strand, within yaiB at 400.782 kb on + strand, within yaiB at 400.783 kb on + strand, within yaiB at 400.783 kb on + strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.783 kb on - strand, within yaiB at 400.812 kb on + strand, within yaiB at 400.812 kb on - strand, within yaiB at 400.815 kb on + strand, within yaiB at 400.815 kb on + strand, within yaiB at 400.816 kb on - strand, within yaiB at 400.823 kb on - strand, within yaiB at 400.828 kb on - strand, within yaiB at 400.835 kb on + strand, within yaiB at 400.865 kb on - strand at 400.898 kb on - strand at 400.899 kb on + strand at 400.901 kb on - strand at 400.901 kb on - strand at 400.915 kb on + strand at 400.915 kb on + strand at 400.982 kb on - strand at 400.992 kb on - strand at 400.998 kb on - strand at 401.004 kb on + strand at 401.006 kb on - strand at 401.006 kb on - strand at 401.015 kb on - strand at 401.015 kb on - strand at 401.020 kb on - strand at 401.078 kb on - strand at 401.086 kb on - strand at 401.088 kb on - strand at 401.088 kb on - strand at 401.091 kb on - strand at 401.091 kb on - strand at 401.105 kb on + strand at 401.113 kb on - strand, within phoA at 401.113 kb on - strand, within phoA at 401.113 kb on - strand, within phoA at 401.120 kb on + strand, within phoA at 401.123 kb on + strand, within phoA at 401.123 kb on + strand, within phoA at 401.125 kb on + strand, within phoA at 401.126 kb on - strand, within phoA at 401.133 kb on - strand, within phoA at 401.192 kb on - strand, within phoA at 401.199 kb on - strand, within phoA at 401.217 kb on - strand, within phoA at 401.271 kb on - strand, within phoA at 401.280 kb on + strand, within phoA at 401.282 kb on + strand, within phoA at 401.290 kb on - strand, within phoA at 401.332 kb on - strand, within phoA at 401.348 kb on + strand, within phoA at 401.348 kb on + strand, within phoA at 401.350 kb on - strand, within phoA at 401.350 kb on - strand, within phoA at 401.350 kb on - strand, within phoA at 401.363 kb on + strand, within phoA at 401.380 kb on + strand, within phoA at 401.486 kb on + strand, within phoA at 401.486 kb on + strand, within phoA at 401.494 kb on - strand, within phoA at 401.503 kb on + strand, within phoA at 401.616 kb on + strand, within phoA at 401.621 kb on + strand, within phoA at 401.653 kb on - strand, within phoA at 401.800 kb on + strand, within phoA at 401.800 kb on + strand, within phoA at 401.808 kb on - strand, within phoA at 401.808 kb on - strand, within phoA at 401.830 kb on - strand, within phoA at 401.839 kb on + strand, within phoA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 399,659 - ddlA b0381 0.55 +0.4 399,707 - ddlA b0381 0.60 +0.6 399,727 + ddlA b0381 0.62 +0.1 399,727 + ddlA b0381 0.62 +0.2 399,787 + ddlA b0381 0.67 +0.7 399,824 + ddlA b0381 0.70 +0.0 399,916 + ddlA b0381 0.79 +0.8 399,933 + ddlA b0381 0.80 +0.6 399,948 + ddlA b0381 0.82 +1.2 399,948 + ddlA b0381 0.82 +0.5 400,092 + +2.3 400,092 + +1.6 400,093 + +2.9 400,093 + +1.0 400,168 + +0.5 400,178 - +0.6 400,240 + +0.5 400,240 + +1.0 400,251 + -0.5 400,258 + +0.4 400,263 + -0.3 400,264 + +1.0 400,282 - +0.2 400,288 - -1.5 400,373 + -0.5 400,559 - +2.6 400,561 + +1.3 400,638 - yaiB b0382 0.11 +1.4 400,667 + yaiB b0382 0.22 +0.7 400,675 + yaiB b0382 0.25 +0.7 400,684 + yaiB b0382 0.28 +1.9 400,692 - yaiB b0382 0.31 +2.2 400,692 - yaiB b0382 0.31 +1.7 400,692 - yaiB b0382 0.31 +1.3 400,707 + yaiB b0382 0.37 +0.6 400,715 - yaiB b0382 0.40 -0.7 400,750 + yaiB b0382 0.54 +1.4 400,762 + yaiB b0382 0.58 +1.4 400,762 + yaiB b0382 0.58 +1.2 400,762 + yaiB b0382 0.58 +1.5 400,770 - yaiB b0382 0.61 -0.0 400,770 - yaiB b0382 0.61 +0.9 400,775 + yaiB b0382 0.63 +1.4 400,777 + yaiB b0382 0.64 +1.3 400,777 + yaiB b0382 0.64 +1.4 400,777 + yaiB b0382 0.64 +0.3 400,777 + yaiB b0382 0.64 +2.1 400,782 + yaiB b0382 0.66 +1.2 400,783 + yaiB b0382 0.66 +1.5 400,783 + yaiB b0382 0.66 +0.3 400,783 - yaiB b0382 0.66 +3.1 400,783 - yaiB b0382 0.66 -0.8 400,783 - yaiB b0382 0.66 +1.7 400,812 + yaiB b0382 0.77 +0.1 400,812 - yaiB b0382 0.77 +2.3 400,815 + yaiB b0382 0.79 +0.4 400,815 + yaiB b0382 0.79 +0.3 400,816 - yaiB b0382 0.79 +1.6 400,823 - yaiB b0382 0.82 +3.0 400,828 - yaiB b0382 0.84 +0.0 400,835 + yaiB b0382 0.86 +1.0 400,865 - +2.3 400,898 - -0.5 400,899 + +0.8 400,901 - -0.5 400,901 - +1.7 400,915 + -0.0 400,915 + -0.2 400,982 - +0.2 400,992 - +0.5 400,998 - +0.1 401,004 + +1.7 401,006 - +1.2 401,006 - +1.8 401,015 - +0.2 401,015 - +0.6 401,020 - +0.8 401,078 - +0.4 401,086 - +0.5 401,088 - +0.4 401,088 - +1.0 401,091 - +0.6 401,091 - +0.2 401,105 + +0.3 401,113 - phoA b0383 0.10 +0.4 401,113 - phoA b0383 0.10 -0.5 401,113 - phoA b0383 0.10 +0.1 401,120 + phoA b0383 0.11 +0.6 401,123 + phoA b0383 0.11 +0.2 401,123 + phoA b0383 0.11 +0.4 401,125 + phoA b0383 0.11 +0.4 401,126 - phoA b0383 0.11 +0.4 401,133 - phoA b0383 0.11 -1.3 401,192 - phoA b0383 0.16 +0.6 401,199 - phoA b0383 0.16 +0.3 401,217 - phoA b0383 0.17 +0.2 401,271 - phoA b0383 0.21 +0.1 401,280 + phoA b0383 0.22 +0.8 401,282 + phoA b0383 0.22 +0.8 401,290 - phoA b0383 0.23 -0.2 401,332 - phoA b0383 0.25 +0.1 401,348 + phoA b0383 0.27 +0.3 401,348 + phoA b0383 0.27 +1.1 401,350 - phoA b0383 0.27 +1.0 401,350 - phoA b0383 0.27 -0.4 401,350 - phoA b0383 0.27 +0.7 401,363 + phoA b0383 0.28 -0.2 401,380 + phoA b0383 0.29 -0.0 401,486 + phoA b0383 0.36 +0.8 401,486 + phoA b0383 0.36 -0.1 401,494 - phoA b0383 0.37 -0.2 401,503 + phoA b0383 0.38 -0.3 401,616 + phoA b0383 0.46 -0.1 401,621 + phoA b0383 0.46 -0.1 401,653 - phoA b0383 0.48 +0.4 401,800 + phoA b0383 0.59 +0.2 401,800 + phoA b0383 0.59 +0.5 401,808 - phoA b0383 0.59 +0.5 401,808 - phoA b0383 0.59 +0.1 401,830 - phoA b0383 0.61 +1.1 401,839 + phoA b0383 0.61 -0.2
Or see this region's nucleotide sequence