Strain Fitness in Escherichia coli BW25113 around b0273

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyagH and yagI are separated by 4 nucleotidesyagI and argF are separated by 138 nucleotidesargF and ykgS are separated by 123 nucleotidesykgS and insB-3 are separated by 15 nucleotidesinsB-3 and insA-3 overlap by 82 nucleotides b0271: yagH - CP4-6 prophage; predicted xylosidase/arabinosidase (NCBI), at 286,013 to 287,623 yagH b0272: yagI - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 287,628 to 288,386 yagI b0273: argF - CP4-6 prophage; ornithine carbamoyltransferase 2, chain F (NCBI), at 288,525 to 289,529 argF b4688: ykgS - no description, at 289,653 to 289,857 ykgS b0274: insB-3 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 289,873 to 290,376 insB-3 b0275: insA-3 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 290,295 to 290,570 insA-3 Position (kb) 288 289 290Strain fitness (log2 ratio) -2 -1 0 1at 287.599 kb on - strandat 287.604 kb on + strandat 287.643 kb on - strandat 287.643 kb on - strandat 287.670 kb on - strandat 287.743 kb on - strand, within yagIat 287.766 kb on + strand, within yagIat 287.782 kb on + strand, within yagIat 287.870 kb on + strand, within yagIat 287.904 kb on + strand, within yagIat 287.914 kb on + strand, within yagIat 287.914 kb on + strand, within yagIat 287.955 kb on + strand, within yagIat 288.049 kb on + strand, within yagIat 288.049 kb on + strand, within yagIat 288.056 kb on - strand, within yagIat 288.056 kb on - strand, within yagIat 288.057 kb on - strand, within yagIat 288.057 kb on - strand, within yagIat 288.103 kb on + strand, within yagIat 288.271 kb on + strand, within yagIat 288.271 kb on + strand, within yagIat 288.274 kb on + strand, within yagIat 288.282 kb on - strand, within yagIat 288.282 kb on - strand, within yagIat 288.308 kb on - strand, within yagIat 288.339 kb on - strandat 288.470 kb on - strandat 288.470 kb on - strandat 288.476 kb on + strandat 288.480 kb on - strandat 288.501 kb on - strandat 288.501 kb on - strandat 288.536 kb on - strandat 288.592 kb on - strandat 288.617 kb on - strandat 288.750 kb on + strand, within argFat 288.750 kb on + strand, within argFat 288.758 kb on - strand, within argFat 288.809 kb on - strand, within argFat 288.820 kb on + strand, within argFat 288.820 kb on + strand, within argFat 289.058 kb on - strand, within argFat 289.099 kb on + strand, within argFat 289.099 kb on - strand, within argFat 289.107 kb on - strand, within argFat 289.107 kb on - strand, within argFat 289.193 kb on + strand, within argFat 289.193 kb on + strand, within argFat 289.207 kb on + strand, within argFat 289.207 kb on + strand, within argFat 289.480 kb on - strandat 289.483 kb on - strandat 289.636 kb on - strandat 289.687 kb on - strand, within ykgS

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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287,599 - -0.1
287,604 + -0.2
287,643 - -0.0
287,643 - +0.2
287,670 - +0.6
287,743 - yagI b0272 0.15 -0.7
287,766 + yagI b0272 0.18 -0.0
287,782 + yagI b0272 0.20 +0.2
287,870 + yagI b0272 0.32 -0.3
287,904 + yagI b0272 0.36 +1.2
287,914 + yagI b0272 0.38 -1.3
287,914 + yagI b0272 0.38 -1.3
287,955 + yagI b0272 0.43 -0.0
288,049 + yagI b0272 0.55 -0.0
288,049 + yagI b0272 0.55 +0.5
288,056 - yagI b0272 0.56 +0.9
288,056 - yagI b0272 0.56 +0.3
288,057 - yagI b0272 0.57 -2.0
288,057 - yagI b0272 0.57 +0.0
288,103 + yagI b0272 0.63 +0.5
288,271 + yagI b0272 0.85 -0.2
288,271 + yagI b0272 0.85 +0.6
288,274 + yagI b0272 0.85 +0.2
288,282 - yagI b0272 0.86 -0.4
288,282 - yagI b0272 0.86 +0.2
288,308 - yagI b0272 0.90 +1.1
288,339 - +0.4
288,470 - -0.0
288,470 - +0.5
288,476 + -0.1
288,480 - -0.1
288,501 - -0.7
288,501 - +0.3
288,536 - -0.2
288,592 - -0.3
288,617 - +0.6
288,750 + argF b0273 0.22 -0.4
288,750 + argF b0273 0.22 -0.1
288,758 - argF b0273 0.23 -0.9
288,809 - argF b0273 0.28 -0.2
288,820 + argF b0273 0.29 +0.1
288,820 + argF b0273 0.29 +0.2
289,058 - argF b0273 0.53 +0.5
289,099 + argF b0273 0.57 -0.6
289,099 - argF b0273 0.57 +0.6
289,107 - argF b0273 0.58 -0.5
289,107 - argF b0273 0.58 +0.9
289,193 + argF b0273 0.66 +0.0
289,193 + argF b0273 0.66 +0.5
289,207 + argF b0273 0.68 +0.4
289,207 + argF b0273 0.68 -0.9
289,480 - -0.2
289,483 - +0.7
289,636 - +0.6
289,687 - ykgS b4688 0.17 +0.0

Or see this region's nucleotide sequence