Strain Fitness in Escherichia coli BW25113 around b2379

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntddg and ypdK are separated by 355 nucleotidesypdK and yfdZ are separated by 64 nucleotidesyfdZ and ypdA are separated by 375 nucleotides b2378: ddg - putative heat shock protein (VIMSS), at 2,493,667 to 2,494,587 ddg b4680: ypdK - expressed protein (RefSeq), at 2,494,943 to 2,495,014 ypdK b2379: yfdZ - hypothetical protein (NCBI), at 2,495,079 to 2,496,317 yfdZ b2380: ypdA - predicted sensory kinase in two-component system with YpdB (NCBI), at 2,496,693 to 2,498,390 ypdA Position (kb) 2495 2496 2497Strain fitness (log2 ratio) -1 0 1at 2494.277 kb on - strand, within ddgat 2494.430 kb on + strand, within ddgat 2494.446 kb on + strand, within ddgat 2494.446 kb on + strand, within ddgat 2494.522 kb on - strandat 2494.524 kb on + strandat 2494.527 kb on + strandat 2494.527 kb on + strandat 2494.527 kb on + strandat 2494.769 kb on + strandat 2494.795 kb on - strandat 2494.815 kb on - strandat 2494.883 kb on + strandat 2494.968 kb on + strand, within ypdKat 2495.161 kb on - strandat 2495.163 kb on - strandat 2495.163 kb on - strandat 2495.165 kb on + strandat 2495.358 kb on + strand, within yfdZat 2495.371 kb on + strand, within yfdZat 2495.404 kb on + strand, within yfdZat 2495.412 kb on - strand, within yfdZat 2495.412 kb on - strand, within yfdZat 2495.444 kb on - strand, within yfdZat 2495.444 kb on - strand, within yfdZat 2495.480 kb on - strand, within yfdZat 2495.480 kb on - strand, within yfdZat 2495.682 kb on + strand, within yfdZat 2495.690 kb on - strand, within yfdZat 2495.865 kb on + strand, within yfdZat 2496.012 kb on - strand, within yfdZat 2496.051 kb on + strand, within yfdZat 2496.139 kb on - strand, within yfdZat 2496.247 kb on - strandat 2496.247 kb on - strandat 2496.282 kb on - strandat 2496.296 kb on + strandat 2496.348 kb on - strandat 2496.348 kb on - strandat 2496.448 kb on - strandat 2496.570 kb on + strandat 2496.570 kb on + strandat 2496.579 kb on - strandat 2496.655 kb on + strandat 2496.659 kb on + strandat 2496.687 kb on - strandat 2496.780 kb on - strandat 2496.814 kb on - strandat 2496.863 kb on + strand, within ypdAat 2496.863 kb on + strand, within ypdAat 2496.954 kb on + strand, within ypdAat 2497.033 kb on + strandat 2497.033 kb on + strand, within ypdAat 2497.065 kb on - strand, within ypdAat 2497.135 kb on - strand, within ypdA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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2,494,277 - ddg b2378 0.66 -0.3
2,494,430 + ddg b2378 0.83 -0.1
2,494,446 + ddg b2378 0.85 -0.5
2,494,446 + ddg b2378 0.85 -0.6
2,494,522 - -0.1
2,494,524 + +0.2
2,494,527 + +0.4
2,494,527 + +0.1
2,494,527 + -0.1
2,494,769 + +0.7
2,494,795 - +1.2
2,494,815 - +0.2
2,494,883 + -0.1
2,494,968 + ypdK b4680 0.35 -0.0
2,495,161 - -0.5
2,495,163 - -0.3
2,495,163 - -0.8
2,495,165 + +0.1
2,495,358 + yfdZ b2379 0.23 -1.2
2,495,371 + yfdZ b2379 0.24 -0.7
2,495,404 + yfdZ b2379 0.26 -1.0
2,495,412 - yfdZ b2379 0.27 -0.5
2,495,412 - yfdZ b2379 0.27 -0.8
2,495,444 - yfdZ b2379 0.29 -1.0
2,495,444 - yfdZ b2379 0.29 -0.3
2,495,480 - yfdZ b2379 0.32 -1.5
2,495,480 - yfdZ b2379 0.32 +0.3
2,495,682 + yfdZ b2379 0.49 -0.8
2,495,690 - yfdZ b2379 0.49 -0.0
2,495,865 + yfdZ b2379 0.63 -0.4
2,496,012 - yfdZ b2379 0.75 -0.9
2,496,051 + yfdZ b2379 0.78 -0.5
2,496,139 - yfdZ b2379 0.86 -0.9
2,496,247 - -0.3
2,496,247 - -0.5
2,496,282 - -0.3
2,496,296 + +0.0
2,496,348 - -0.5
2,496,348 - -0.9
2,496,448 - +0.1
2,496,570 + -0.2
2,496,570 + -0.6
2,496,579 - +0.3
2,496,655 + -0.3
2,496,659 + +0.3
2,496,687 - -0.2
2,496,780 - +0.2
2,496,814 - -0.2
2,496,863 + ypdA b2380 0.10 -1.0
2,496,863 + ypdA b2380 0.10 +0.3
2,496,954 + ypdA b2380 0.15 -0.9
2,497,033 + +1.3
2,497,033 + ypdA b2380 0.20 -1.4
2,497,065 - ypdA b2380 0.22 -0.3
2,497,135 - ypdA b2380 0.26 +0.6

Or see this region's nucleotide sequence