Strain Fitness in Escherichia coli BW25113 around b1948

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliM and fliN overlap by 4 nucleotidesfliN and fliO are separated by 2 nucleotidesfliO and fliP overlap by 1 nucleotidesfliP and fliQ are separated by 9 nucleotidesfliQ and fliR are separated by 7 nucleotides b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115 fliM b1946: fliN - flagellar motor switch protein (NCBI), at 2,019,112 to 2,019,525 fliN b1947: fliO - flagellar biosynthesis (VIMSS), at 2,019,528 to 2,019,893 fliO b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630 fliP b1949: fliQ - flagellar biosynthesis protein Q (NCBI), at 2,020,640 to 2,020,909 fliQ b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702 fliR Position (kb) 2019 2020 2021Strain fitness (log2 ratio) -1 0 1 2at 2019.025 kb on + strandat 2019.106 kb on + strandat 2019.397 kb on + strand, within fliNat 2019.437 kb on + strand, within fliNat 2019.538 kb on + strandat 2019.538 kb on + strandat 2019.995 kb on + strand, within fliPat 2020.000 kb on + strand, within fliPat 2020.231 kb on - strand, within fliPat 2020.359 kb on - strand, within fliPat 2020.359 kb on - strand, within fliPat 2020.375 kb on + strand, within fliPat 2020.382 kb on - strand, within fliPat 2020.668 kb on + strand, within fliQat 2020.719 kb on - strand, within fliQat 2020.840 kb on + strand, within fliQat 2020.894 kb on + strandat 2020.899 kb on - strandat 2020.950 kb on - strandat 2021.298 kb on + strand, within fliRat 2021.377 kb on + strand, within fliRat 2021.377 kb on + strand, within fliRat 2021.413 kb on - strand, within fliRat 2021.498 kb on + strand, within fliRat 2021.501 kb on + strand, within fliRat 2021.501 kb on + strand, within fliRat 2021.522 kb on - strand, within fliRat 2021.572 kb on + strand, within fliRat 2021.594 kb on + strand, within fliRat 2021.594 kb on + strand, within fliRat 2021.595 kb on + strand, within fliRat 2021.595 kb on + strand, within fliR

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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2,019,025 + +0.1
2,019,106 + +0.2
2,019,397 + fliN b1946 0.69 +0.2
2,019,437 + fliN b1946 0.79 +0.7
2,019,538 + +0.6
2,019,538 + +0.8
2,019,995 + fliP b1948 0.14 +0.2
2,020,000 + fliP b1948 0.14 +0.4
2,020,231 - fliP b1948 0.46 +0.0
2,020,359 - fliP b1948 0.63 +0.0
2,020,359 - fliP b1948 0.63 +0.0
2,020,375 + fliP b1948 0.65 +0.5
2,020,382 - fliP b1948 0.66 +0.6
2,020,668 + fliQ b1949 0.10 +0.8
2,020,719 - fliQ b1949 0.29 +0.4
2,020,840 + fliQ b1949 0.74 -0.1
2,020,894 + -0.2
2,020,899 - +0.3
2,020,950 - +0.7
2,021,298 + fliR b1950 0.48 +0.0
2,021,377 + fliR b1950 0.59 +1.2
2,021,377 + fliR b1950 0.59 -0.6
2,021,413 - fliR b1950 0.63 +0.5
2,021,498 + fliR b1950 0.74 -0.5
2,021,501 + fliR b1950 0.74 +0.5
2,021,501 + fliR b1950 0.74 +0.6
2,021,522 - fliR b1950 0.77 +2.0
2,021,572 + fliR b1950 0.83 +0.5
2,021,594 + fliR b1950 0.86 -0.5
2,021,594 + fliR b1950 0.86 +0.0
2,021,595 + fliR b1950 0.86 -1.1
2,021,595 + fliR b1950 0.86 +0.2

Or see this region's nucleotide sequence