Strain Fitness in Escherichia coli BW25113 around b1848

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebE and yebF are separated by 326 nucleotidesyebF and yebG are separated by 66 nucleotidesyebG and purT are separated by 133 nucleotides b1846: yebE - hypothetical protein (NCBI), at 1,927,072 to 1,927,731 yebE b1847: yebF - predicted protein (RefSeq), at 1,928,058 to 1,928,414 yebF b1848: yebG - conserved protein regulated by LexA (NCBI), at 1,928,481 to 1,928,771 yebG b1849: purT - phosphoribosylglycinamide formyltransferase 2 (NCBI), at 1,928,905 to 1,930,083 purT Position (kb) 1928 1929Strain fitness (log2 ratio) -2 -1 0 1at 1927.533 kb on + strand, within yebEat 1927.578 kb on + strand, within yebEat 1927.767 kb on - strandat 1927.861 kb on + strandat 1927.896 kb on + strandat 1928.014 kb on - strandat 1928.054 kb on + strandat 1928.054 kb on + strandat 1928.324 kb on + strand, within yebFat 1928.324 kb on + strand, within yebFat 1928.426 kb on + strandat 1928.426 kb on + strandat 1928.449 kb on - strandat 1928.449 kb on - strandat 1928.740 kb on - strand, within yebGat 1928.981 kb on - strandat 1928.981 kb on - strandat 1928.981 kb on - strandat 1929.008 kb on + strandat 1929.150 kb on - strand, within purTat 1929.362 kb on + strand, within purTat 1929.362 kb on + strand, within purTat 1929.640 kb on - strand, within purTat 1929.640 kb on - strand, within purTat 1929.683 kb on + strand, within purTat 1929.683 kb on + strand, within purTat 1929.759 kb on - strand, within purT

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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1,927,533 + yebE b1846 0.70 +0.4
1,927,578 + yebE b1846 0.77 -2.5
1,927,767 - +0.2
1,927,861 + +0.4
1,927,896 + +1.0
1,928,014 - +1.0
1,928,054 + -2.4
1,928,054 + +1.5
1,928,324 + yebF b1847 0.75 -0.6
1,928,324 + yebF b1847 0.75 -0.1
1,928,426 + -0.4
1,928,426 + +0.3
1,928,449 - +0.1
1,928,449 - +0.2
1,928,740 - yebG b1848 0.89 +0.5
1,928,981 - +1.0
1,928,981 - +0.4
1,928,981 - -0.6
1,929,008 + +0.1
1,929,150 - purT b1849 0.21 +0.1
1,929,362 + purT b1849 0.39 +0.4
1,929,362 + purT b1849 0.39 -0.5
1,929,640 - purT b1849 0.62 -1.0
1,929,640 - purT b1849 0.62 -0.1
1,929,683 + purT b1849 0.66 -0.1
1,929,683 + purT b1849 0.66 -0.2
1,929,759 - purT b1849 0.72 +0.0

Or see this region's nucleotide sequence