Strain Fitness in Escherichia coli BW25113 around b1752

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydjX and ydjY are separated by 4 nucleotidesydjY and ydjZ are separated by 14 nucleotidesydjZ and ynjA overlap by 1 nucleotidesynjA and b1754 are separated by 9 nucleotides b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135 ydjX b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817 ydjY b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539 ydjZ b1753: ynjA - hypothetical protein (NCBI), at 1,833,539 to 1,834,087 ynjA b1754: b1754 - orf, hypothetical protein (VIMSS), at 1,834,097 to 1,835,263 b1754 Position (kb) 1832 1833 1834Strain fitness (log2 ratio) -2 -1 0 1at 1831.834 kb on + strand, within ydjXat 1831.992 kb on - strand, within ydjXat 1832.016 kb on + strand, within ydjXat 1832.016 kb on + strand, within ydjXat 1832.084 kb on - strandat 1832.113 kb on - strandat 1832.113 kb on - strandat 1832.187 kb on + strandat 1832.403 kb on + strand, within ydjYat 1832.456 kb on + strand, within ydjYat 1832.456 kb on + strand, within ydjYat 1832.456 kb on + strand, within ydjYat 1832.456 kb on + strand, within ydjYat 1832.475 kb on - strand, within ydjYat 1832.475 kb on - strand, within ydjYat 1832.682 kb on + strand, within ydjYat 1832.707 kb on - strand, within ydjYat 1833.245 kb on + strand, within ydjZat 1833.394 kb on - strand, within ydjZat 1833.430 kb on - strand, within ydjZat 1833.789 kb on + strand, within ynjAat 1833.789 kb on + strand, within ynjAat 1834.210 kb on + strandat 1834.332 kb on - strand, within b1754at 1834.427 kb on + strand, within b1754

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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1,831,834 + ydjX b1750 0.58 +0.1
1,831,992 - ydjX b1750 0.80 -0.5
1,832,016 + ydjX b1750 0.83 +0.5
1,832,016 + ydjX b1750 0.83 +0.4
1,832,084 - +0.3
1,832,113 - +0.3
1,832,113 - -0.1
1,832,187 + -0.3
1,832,403 + ydjY b1751 0.39 +0.7
1,832,456 + ydjY b1751 0.47 +0.1
1,832,456 + ydjY b1751 0.47 +0.5
1,832,456 + ydjY b1751 0.47 +0.9
1,832,456 + ydjY b1751 0.47 +0.6
1,832,475 - ydjY b1751 0.49 -0.4
1,832,475 - ydjY b1751 0.49 +0.1
1,832,682 + ydjY b1751 0.80 +0.1
1,832,707 - ydjY b1751 0.84 -0.3
1,833,245 + ydjZ b1752 0.58 +0.4
1,833,394 - ydjZ b1752 0.79 -0.1
1,833,430 - ydjZ b1752 0.84 +0.6
1,833,789 + ynjA b1753 0.46 -0.2
1,833,789 + ynjA b1753 0.46 +0.6
1,834,210 + -0.1
1,834,332 - b1754 b1754 0.20 -2.6
1,834,427 + b1754 b1754 0.28 -0.4

Or see this region's nucleotide sequence