Strain Fitness in Escherichia coli BW25113 around b1648

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhF and b1648 are separated by 48 nucleotidesb1648 and ydhM are separated by 102 nucleotidesydhM and nemA are separated by 36 nucleotides b1647: ydhF - predicted oxidoreductase (NCBI), at 1,722,760 to 1,723,656 ydhF b1648: b1648 - orf, hypothetical protein (VIMSS), at 1,723,705 to 1,723,944 b1648 b1649: ydhM - predicted DNA-binding transcriptional regulator (RefSeq), at 1,724,047 to 1,724,646 ydhM b1650: nemA - N-ethylmaleimide reductase, FMN-linked (NCBI), at 1,724,683 to 1,725,780 nemA Position (kb) 1723 1724Strain fitness (log2 ratio) -1 0 1at 1722.868 kb on - strand, within ydhFat 1722.868 kb on - strand, within ydhFat 1722.904 kb on - strand, within ydhFat 1722.904 kb on - strand, within ydhFat 1723.132 kb on + strand, within ydhFat 1723.138 kb on - strand, within ydhFat 1723.490 kb on + strand, within ydhFat 1723.797 kb on - strand, within b1648at 1724.028 kb on + strandat 1724.772 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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1,722,868 - ydhF b1647 0.12 -0.0
1,722,868 - ydhF b1647 0.12 -0.2
1,722,904 - ydhF b1647 0.16 +0.4
1,722,904 - ydhF b1647 0.16 -0.2
1,723,132 + ydhF b1647 0.41 +0.3
1,723,138 - ydhF b1647 0.42 +0.4
1,723,490 + ydhF b1647 0.81 -0.2
1,723,797 - b1648 b1648 0.38 +0.3
1,724,028 + +0.2
1,724,772 - +0.1

Or see this region's nucleotide sequence