Strain Fitness in Escherichia coli BW25113 around b1619

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuidR and hdhA are separated by 220 nucleotideshdhA and malI are separated by 111 nucleotides b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076 uidR b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064 hdhA b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204 malI Position (kb) 1695 1696 1697Strain fitness (log2 ratio) -2 -1 0 1 2at 1694.629 kb on + strandat 1694.629 kb on + strand, within uidRat 1694.746 kb on - strand, within uidRat 1694.873 kb on + strand, within uidRat 1694.873 kb on + strand, within uidRat 1694.938 kb on + strand, within uidRat 1695.083 kb on + strandat 1695.089 kb on - strandat 1695.234 kb on - strandat 1695.316 kb on - strandat 1695.368 kb on + strandat 1695.368 kb on + strandat 1695.393 kb on - strand, within hdhAat 1695.425 kb on + strand, within hdhAat 1695.432 kb on - strand, within hdhAat 1695.432 kb on - strand, within hdhAat 1695.558 kb on - strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.582 kb on - strand, within hdhAat 1695.582 kb on - strand, within hdhAat 1695.592 kb on - strand, within hdhAat 1695.592 kb on - strand, within hdhAat 1695.690 kb on - strand, within hdhAat 1695.690 kb on - strand, within hdhAat 1695.724 kb on - strand, within hdhAat 1695.758 kb on - strand, within hdhAat 1695.771 kb on - strand, within hdhAat 1695.771 kb on - strand, within hdhAat 1695.779 kb on - strand, within hdhAat 1695.779 kb on - strand, within hdhAat 1695.841 kb on - strand, within hdhAat 1695.841 kb on - strand, within hdhAat 1695.901 kb on - strand, within hdhAat 1695.914 kb on - strand, within hdhAat 1695.925 kb on - strand, within hdhAat 1695.959 kb on + strand, within hdhAat 1695.959 kb on + strand, within hdhAat 1696.001 kb on - strandat 1696.125 kb on - strandat 1696.125 kb on - strandat 1696.151 kb on - strandat 1696.154 kb on + strandat 1696.154 kb on + strandat 1696.154 kb on + strandat 1696.161 kb on - strandat 1696.161 kb on - strandat 1696.163 kb on - strandat 1696.163 kb on - strandat 1696.176 kb on + strandat 1696.176 kb on + strandat 1696.181 kb on - strandat 1696.181 kb on - strandat 1696.314 kb on + strand, within malIat 1696.330 kb on - strand, within malIat 1696.599 kb on - strand, within malIat 1696.617 kb on + strand, within malIat 1696.643 kb on + strand, within malI

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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1,694,629 + +0.1
1,694,629 + uidR b1618 0.24 +0.5
1,694,746 - uidR b1618 0.44 -0.1
1,694,873 + uidR b1618 0.65 +0.5
1,694,873 + uidR b1618 0.65 -0.2
1,694,938 + uidR b1618 0.76 -0.2
1,695,083 + +0.0
1,695,089 - -0.1
1,695,234 - +0.2
1,695,316 - -0.1
1,695,368 + -0.3
1,695,368 + -0.0
1,695,393 - hdhA b1619 0.12 -0.4
1,695,425 + hdhA b1619 0.17 +0.4
1,695,432 - hdhA b1619 0.18 +1.0
1,695,432 - hdhA b1619 0.18 +0.7
1,695,558 - hdhA b1619 0.34 -0.3
1,695,574 + hdhA b1619 0.36 +1.8
1,695,574 + hdhA b1619 0.36 -0.2
1,695,574 + hdhA b1619 0.36 +0.4
1,695,582 - hdhA b1619 0.37 +0.4
1,695,582 - hdhA b1619 0.37 +0.6
1,695,592 - hdhA b1619 0.38 +0.4
1,695,592 - hdhA b1619 0.38 -0.2
1,695,690 - hdhA b1619 0.51 +0.9
1,695,690 - hdhA b1619 0.51 +0.2
1,695,724 - hdhA b1619 0.56 -0.7
1,695,758 - hdhA b1619 0.60 -0.0
1,695,771 - hdhA b1619 0.62 -1.1
1,695,771 - hdhA b1619 0.62 +0.2
1,695,779 - hdhA b1619 0.63 +0.7
1,695,779 - hdhA b1619 0.63 -0.0
1,695,841 - hdhA b1619 0.71 +0.2
1,695,841 - hdhA b1619 0.71 -1.0
1,695,901 - hdhA b1619 0.79 +0.8
1,695,914 - hdhA b1619 0.80 +1.0
1,695,925 - hdhA b1619 0.82 +1.8
1,695,959 + hdhA b1619 0.86 +0.6
1,695,959 + hdhA b1619 0.86 +1.9
1,696,001 - +0.7
1,696,125 - -0.3
1,696,125 - +0.3
1,696,151 - -0.4
1,696,154 + -0.1
1,696,154 + +1.6
1,696,154 + -1.2
1,696,161 - -0.6
1,696,161 - +0.0
1,696,163 - +0.2
1,696,163 - +0.0
1,696,176 + -2.5
1,696,176 + +0.5
1,696,181 - +0.4
1,696,181 - -0.8
1,696,314 + malI b1620 0.13 +0.1
1,696,330 - malI b1620 0.15 -0.9
1,696,599 - malI b1620 0.41 +0.1
1,696,617 + malI b1620 0.43 +0.7
1,696,643 + malI b1620 0.45 -0.2

Or see this region's nucleotide sequence