Experiment: Ying_all64 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folC and accD are separated by 69 nucleotides accD and dedA are separated by 155 nucleotides dedA and truA are separated by 82 nucleotides
b2315: folC - bifunctional folylpolyglutamate synthase/ dihydrofolate synthase (NCBI), at 2,429,696 to 2,430,964
folC
b2316: accD - acetyl-CoA carboxylase subunit beta (NCBI), at 2,431,034 to 2,431,948
accD
b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763
dedA
b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658
truA
Position (kb)
2431
2432 Strain fitness (log2 ratio)
-1
0
1 at 2430.901 kb on - strand at 2430.958 kb on - strand at 2430.998 kb on - strand at 2430.998 kb on - strand at 2431.014 kb on - strand at 2431.015 kb on - strand at 2431.015 kb on - strand at 2431.016 kb on - strand at 2432.025 kb on - strand at 2432.025 kb on - strand at 2432.196 kb on + strand, within dedA at 2432.269 kb on - strand, within dedA at 2432.269 kb on - strand, within dedA at 2432.290 kb on - strand, within dedA at 2432.290 kb on - strand, within dedA at 2432.494 kb on + strand, within dedA at 2432.650 kb on + strand, within dedA at 2432.650 kb on + strand, within dedA at 2432.800 kb on + strand at 2432.808 kb on - strand at 2432.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3 remove 2,430,901 - -0.5 2,430,958 - +0.1 2,430,998 - +0.0 2,430,998 - +0.3 2,431,014 - -0.0 2,431,015 - -0.2 2,431,015 - +0.4 2,431,016 - -0.6 2,432,025 - +0.3 2,432,025 - +0.1 2,432,196 + dedA b2317 0.14 -0.5 2,432,269 - dedA b2317 0.25 +0.3 2,432,269 - dedA b2317 0.25 -0.1 2,432,290 - dedA b2317 0.28 +0.0 2,432,290 - dedA b2317 0.28 +0.2 2,432,494 + dedA b2317 0.59 +0.3 2,432,650 + dedA b2317 0.83 +0.1 2,432,650 + dedA b2317 0.83 +0.4 2,432,800 + -0.5 2,432,808 - +0.2 2,432,808 - -0.3
Or see this region's nucleotide sequence