Strain Fitness in Escherichia coli BW25113 around b1266

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpE and trpL are separated by 91 nucleotidestrpL and yciV are separated by 137 nucleotidesyciV and yciO overlap by 4 nucleotidesyciO and yciQ are separated by 27 nucleotides b1264: trpE - anthranilate synthase component I (NCBI), at 1,319,408 to 1,320,970 trpE b1265: trpL - trp operon leader peptide (NCBI), at 1,321,062 to 1,321,106 trpL b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125 yciV b1267: yciO - orf, hypothetical protein (VIMSS), at 1,322,122 to 1,322,742 yciO b1268: yciQ - predicted inner membrane protein (NCBI), at 1,322,770 to 1,324,665 yciQ Position (kb) 1321 1322 1323Strain fitness (log2 ratio) -1 0 1 2at 1320.275 kb on - strand, within trpEat 1320.275 kb on - strand, within trpEat 1320.672 kb on - strand, within trpEat 1320.809 kb on - strand, within trpEat 1320.890 kb on - strandat 1321.053 kb on - strandat 1321.053 kb on - strandat 1321.257 kb on + strandat 1321.332 kb on + strandat 1321.332 kb on + strandat 1321.378 kb on + strand, within yciVat 1321.381 kb on + strand, within yciVat 1321.537 kb on - strand, within yciVat 1321.537 kb on - strand, within yciVat 1321.697 kb on + strand, within yciVat 1321.935 kb on - strand, within yciVat 1322.096 kb on + strandat 1322.096 kb on + strandat 1322.161 kb on - strandat 1322.231 kb on + strand, within yciOat 1322.708 kb on + strandat 1322.713 kb on + strandat 1322.766 kb on - strandat 1322.814 kb on + strandat 1322.880 kb on + strandat 1322.880 kb on + strandat 1323.017 kb on + strand, within yciQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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1,320,275 - trpE b1264 0.55 -0.1
1,320,275 - trpE b1264 0.55 -0.0
1,320,672 - trpE b1264 0.81 -0.3
1,320,809 - trpE b1264 0.90 +0.3
1,320,890 - +0.5
1,321,053 - -0.5
1,321,053 - +0.1
1,321,257 + +0.2
1,321,332 + -0.8
1,321,332 + -0.1
1,321,378 + yciV b1266 0.15 +0.0
1,321,381 + yciV b1266 0.16 +1.1
1,321,537 - yciV b1266 0.33 -0.4
1,321,537 - yciV b1266 0.33 +0.3
1,321,697 + yciV b1266 0.51 +0.2
1,321,935 - yciV b1266 0.78 +0.3
1,322,096 + -1.6
1,322,096 + +2.1
1,322,161 - -0.2
1,322,231 + yciO b1267 0.18 -0.7
1,322,708 + -0.1
1,322,713 + +0.1
1,322,766 - +0.4
1,322,814 + -0.2
1,322,880 + +0.2
1,322,880 + +0.1
1,323,017 + yciQ b1268 0.13 +0.9

Or see this region's nucleotide sequence