Strain Fitness in Escherichia coli BW25113 around b1259

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciE and yciF are separated by 45 nucleotidesyciF and yciG are separated by 85 nucleotidesyciG and trpA are separated by 380 nucleotides b1257: yciE - hypothetical protein (NCBI), at 1,312,742 to 1,313,248 yciE b1258: yciF - hypothetical protein (NCBI), at 1,313,294 to 1,313,794 yciF b1259: yciG - orf, hypothetical protein (VIMSS), at 1,313,880 to 1,314,059 yciG b1260: trpA - tryptophan synthase subunit alpha (NCBI), at 1,314,440 to 1,315,246 trpA Position (kb) 1313 1314 1315Strain fitness (log2 ratio) -1 0 1at 1312.909 kb on - strand, within yciEat 1313.347 kb on - strand, within yciFat 1313.409 kb on - strand, within yciFat 1313.479 kb on - strand, within yciFat 1313.499 kb on - strand, within yciFat 1313.642 kb on + strand, within yciFat 1313.939 kb on - strand, within yciGat 1313.996 kb on - strand, within yciGat 1314.336 kb on + strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.666 kb on + strand, within trpAat 1314.931 kb on - strand, within trpA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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1,312,909 - yciE b1257 0.33 +0.7
1,313,347 - yciF b1258 0.11 +0.2
1,313,409 - yciF b1258 0.23 +0.3
1,313,479 - yciF b1258 0.37 -0.2
1,313,499 - yciF b1258 0.41 -1.3
1,313,642 + yciF b1258 0.69 +1.7
1,313,939 - yciG b1259 0.33 +0.3
1,313,996 - yciG b1259 0.64 +1.0
1,314,336 + +0.6
1,314,440 - -0.0
1,314,440 - +0.8
1,314,440 - +0.5
1,314,666 + trpA b1260 0.28 -0.2
1,314,931 - trpA b1260 0.61 +0.5

Or see this region's nucleotide sequence