Strain Fitness in Escherichia coli BW25113 around b1220

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntchaC and ychN are separated by 43 nucleotidesychN and ychO are separated by 184 nucleotidesychO and narL are separated by 0 nucleotidesnarL and narX overlap by 8 nucleotides b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425 chaC b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822 ychN b1220: ychO - predicted invasin (RefSeq), at 1,273,007 to 1,274,401 ychO b1221: narL - DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI), at 1,274,402 to 1,275,052 narL b1222: narX - sensory histidine kinase in two-component regulatory system with NarL (NCBI), at 1,275,045 to 1,276,841 narX Position (kb) 1273 1274 1275Strain fitness (log2 ratio) -1 0 1at 1272.143 kb on - strand, within chaCat 1272.159 kb on - strand, within chaCat 1272.288 kb on - strand, within chaCat 1272.522 kb on - strand, within ychNat 1272.532 kb on - strand, within ychNat 1272.581 kb on - strand, within ychNat 1272.615 kb on - strand, within ychNat 1272.714 kb on + strand, within ychNat 1272.787 kb on - strandat 1272.787 kb on - strandat 1273.039 kb on + strandat 1273.305 kb on + strand, within ychOat 1273.328 kb on - strand, within ychOat 1273.328 kb on - strand, within ychOat 1273.463 kb on + strand, within ychOat 1273.493 kb on + strand, within ychOat 1273.732 kb on + strand, within ychOat 1273.737 kb on + strand, within ychOat 1273.930 kb on - strand, within ychOat 1274.003 kb on + strand, within ychOat 1274.343 kb on - strandat 1274.391 kb on + strandat 1274.489 kb on - strand, within narLat 1274.543 kb on + strand, within narLat 1274.543 kb on + strand, within narLat 1274.583 kb on - strand, within narLat 1274.668 kb on - strand, within narLat 1274.677 kb on - strand, within narLat 1274.677 kb on - strand, within narLat 1275.022 kb on + strandat 1275.022 kb on + strandat 1275.035 kb on - strandat 1275.035 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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1,272,143 - chaC b1218 0.59 +0.1
1,272,159 - chaC b1218 0.62 +0.3
1,272,288 - chaC b1218 0.80 +0.1
1,272,522 - ychN b1219 0.15 -0.0
1,272,532 - ychN b1219 0.18 -0.7
1,272,581 - ychN b1219 0.32 +0.2
1,272,615 - ychN b1219 0.41 +0.2
1,272,714 + ychN b1219 0.69 +0.1
1,272,787 - +0.9
1,272,787 - +0.2
1,273,039 + +0.2
1,273,305 + ychO b1220 0.21 +0.4
1,273,328 - ychO b1220 0.23 +0.0
1,273,328 - ychO b1220 0.23 +0.6
1,273,463 + ychO b1220 0.33 +0.1
1,273,493 + ychO b1220 0.35 +0.6
1,273,732 + ychO b1220 0.52 +0.5
1,273,737 + ychO b1220 0.52 +0.2
1,273,930 - ychO b1220 0.66 +0.1
1,274,003 + ychO b1220 0.71 -0.5
1,274,343 - +0.1
1,274,391 + +0.4
1,274,489 - narL b1221 0.13 +0.2
1,274,543 + narL b1221 0.22 -0.1
1,274,543 + narL b1221 0.22 -0.1
1,274,583 - narL b1221 0.28 +0.3
1,274,668 - narL b1221 0.41 -0.3
1,274,677 - narL b1221 0.42 -0.1
1,274,677 - narL b1221 0.42 +0.4
1,275,022 + +1.1
1,275,022 + -0.1
1,275,035 - -0.5
1,275,035 - -0.0

Or see this region's nucleotide sequence