Strain Fitness in Escherichia coli BW25113 around b0631

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlipB and ybeD are separated by 100 nucleotidesybeD and dacA are separated by 109 nucleotides b0630: lipB - protein of lipoate biosynthesis (VIMSS), at 660,860 to 661,501 lipB b0631: ybeD - hypothetical protein (NCBI), at 661,602 to 661,865 ybeD b0632: dacA - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI), at 661,975 to 663,186 dacA Position (kb) 661 662Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 660.730 kb on - strandat 660.850 kb on - strandat 660.850 kb on - strandat 661.084 kb on - strand, within lipBat 661.146 kb on + strand, within lipBat 661.264 kb on + strand, within lipBat 661.509 kb on + strandat 661.519 kb on - strandat 661.957 kb on - strandat 661.957 kb on - strandat 662.131 kb on + strand, within dacAat 662.153 kb on - strand, within dacAat 662.155 kb on + strand, within dacAat 662.160 kb on + strand, within dacAat 662.160 kb on + strand, within dacAat 662.255 kb on + strand, within dacAat 662.380 kb on - strand, within dacAat 662.380 kb on - strand, within dacAat 662.462 kb on - strand, within dacAat 662.462 kb on - strand, within dacAat 662.609 kb on - strand, within dacAat 662.609 kb on - strand, within dacAat 662.618 kb on + strand, within dacAat 662.618 kb on + strand, within dacAat 662.618 kb on + strand, within dacAat 662.618 kb on + strand, within dacAat 662.618 kb on + strand, within dacAat 662.620 kb on + strand, within dacAat 662.644 kb on + strand, within dacAat 662.644 kb on + strand, within dacAat 662.644 kb on + strand, within dacAat 662.644 kb on + strand, within dacAat 662.652 kb on - strand, within dacAat 662.652 kb on - strand, within dacAat 662.864 kb on + strand, within dacA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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660,730 - +2.0
660,850 - +1.0
660,850 - +2.0
661,084 - lipB b0630 0.35 +0.4
661,146 + lipB b0630 0.45 -0.0
661,264 + lipB b0630 0.63 +0.0
661,509 + -0.2
661,519 - +0.1
661,957 - +0.1
661,957 - +0.5
662,131 + dacA b0632 0.13 -3.6
662,153 - dacA b0632 0.15 -2.9
662,155 + dacA b0632 0.15 -0.0
662,160 + dacA b0632 0.15 -0.8
662,160 + dacA b0632 0.15 -2.0
662,255 + dacA b0632 0.23 -2.6
662,380 - dacA b0632 0.33 -2.2
662,380 - dacA b0632 0.33 -3.3
662,462 - dacA b0632 0.40 -2.1
662,462 - dacA b0632 0.40 -2.5
662,609 - dacA b0632 0.52 -2.1
662,609 - dacA b0632 0.52 -3.0
662,618 + dacA b0632 0.53 -2.7
662,618 + dacA b0632 0.53 -2.1
662,618 + dacA b0632 0.53 -3.4
662,618 + dacA b0632 0.53 -4.1
662,618 + dacA b0632 0.53 -3.9
662,620 + dacA b0632 0.53 -3.1
662,644 + dacA b0632 0.55 -2.3
662,644 + dacA b0632 0.55 -3.8
662,644 + dacA b0632 0.55 -2.4
662,644 + dacA b0632 0.55 -2.7
662,652 - dacA b0632 0.56 -1.2
662,652 - dacA b0632 0.56 -3.5
662,864 + dacA b0632 0.73 -3.8

Or see this region's nucleotide sequence