Experiment: Ying_Others16 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsK and rbsR are separated by 3 nucleotides rbsR and hsrA overlap by 35 nucleotides
b3752: rbsK - ribokinase (NCBI), at 3,935,317 to 3,936,246
rbsK
b3753: rbsR - DNA-binding transcriptional repressor of ribose metabolism (NCBI), at 3,936,250 to 3,937,242
rbsR
b3754: hsrA - predicted multidrug or homocysteine efflux system (NCBI), at 3,937,208 to 3,938,635
hsrA
Position (kb)
3936
3937
3938 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 3935.280 kb on + strand at 3935.420 kb on + strand, within rbsK at 3935.420 kb on + strand, within rbsK at 3935.469 kb on - strand, within rbsK at 3935.485 kb on - strand, within rbsK at 3935.493 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.560 kb on - strand, within rbsK at 3935.577 kb on - strand, within rbsK at 3935.650 kb on + strand, within rbsK at 3935.656 kb on - strand, within rbsK at 3935.661 kb on + strand, within rbsK at 3935.666 kb on + strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.711 kb on + strand, within rbsK at 3935.742 kb on + strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.808 kb on + strand, within rbsK at 3935.942 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.955 kb on - strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.983 kb on + strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.009 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.048 kb on + strand, within rbsK at 3936.055 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.082 kb on + strand, within rbsK at 3936.082 kb on + strand, within rbsK at 3936.197 kb on + strand at 3936.338 kb on - strand at 3936.338 kb on - strand at 3936.395 kb on + strand, within rbsR at 3936.407 kb on + strand, within rbsR at 3936.415 kb on - strand, within rbsR at 3936.444 kb on - strand, within rbsR at 3936.447 kb on - strand, within rbsR at 3936.456 kb on + strand, within rbsR at 3936.456 kb on + strand, within rbsR at 3936.463 kb on - strand, within rbsR at 3936.470 kb on + strand, within rbsR at 3936.470 kb on + strand, within rbsR at 3936.473 kb on - strand, within rbsR at 3936.473 kb on - strand, within rbsR at 3936.496 kb on - strand, within rbsR at 3936.496 kb on - strand, within rbsR at 3936.545 kb on + strand, within rbsR at 3936.659 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.667 kb on + strand, within rbsR at 3936.684 kb on - strand, within rbsR at 3936.684 kb on - strand, within rbsR at 3936.686 kb on + strand, within rbsR at 3936.723 kb on - strand, within rbsR at 3936.726 kb on - strand, within rbsR at 3936.763 kb on - strand, within rbsR at 3936.769 kb on + strand, within rbsR at 3936.794 kb on + strand, within rbsR at 3936.842 kb on - strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.868 kb on + strand, within rbsR at 3936.875 kb on + strand, within rbsR at 3936.876 kb on - strand, within rbsR at 3936.876 kb on - strand, within rbsR at 3936.878 kb on + strand, within rbsR at 3936.878 kb on + strand, within rbsR at 3936.943 kb on + strand, within rbsR at 3936.953 kb on - strand, within rbsR at 3936.953 kb on - strand, within rbsR at 3936.989 kb on + strand, within rbsR at 3936.989 kb on + strand, within rbsR at 3936.990 kb on + strand, within rbsR at 3936.992 kb on + strand, within rbsR at 3937.039 kb on + strand, within rbsR at 3937.055 kb on - strand, within rbsR at 3937.101 kb on + strand, within rbsR at 3937.124 kb on - strand, within rbsR at 3937.124 kb on - strand, within rbsR at 3937.130 kb on - strand, within rbsR at 3937.137 kb on + strand, within rbsR at 3937.174 kb on + strand at 3937.174 kb on + strand at 3937.177 kb on - strand at 3937.184 kb on - strand at 3937.186 kb on + strand at 3937.186 kb on + strand at 3937.201 kb on + strand at 3937.205 kb on + strand at 3937.244 kb on - strand at 3937.244 kb on - strand at 3937.312 kb on - strand at 3937.318 kb on - strand at 3937.318 kb on - strand at 3937.342 kb on - strand at 3937.365 kb on + strand, within hsrA at 3937.373 kb on + strand, within hsrA at 3937.406 kb on + strand, within hsrA at 3937.418 kb on + strand, within hsrA at 3937.458 kb on + strand, within hsrA at 3937.458 kb on - strand, within hsrA at 3937.467 kb on + strand, within hsrA at 3937.470 kb on - strand, within hsrA at 3937.470 kb on - strand, within hsrA at 3937.498 kb on + strand, within hsrA at 3937.506 kb on + strand, within hsrA at 3937.506 kb on - strand, within hsrA at 3937.506 kb on - strand, within hsrA at 3937.511 kb on - strand, within hsrA at 3937.523 kb on - strand, within hsrA at 3937.533 kb on - strand, within hsrA at 3937.533 kb on - strand, within hsrA at 3937.541 kb on + strand, within hsrA at 3937.549 kb on - strand, within hsrA at 3937.570 kb on - strand, within hsrA at 3937.570 kb on - strand, within hsrA at 3937.579 kb on - strand, within hsrA at 3937.579 kb on - strand, within hsrA at 3937.592 kb on - strand, within hsrA at 3937.592 kb on - strand, within hsrA at 3937.596 kb on + strand, within hsrA at 3937.630 kb on + strand, within hsrA at 3937.647 kb on - strand, within hsrA at 3937.647 kb on - strand, within hsrA at 3937.651 kb on + strand, within hsrA at 3937.654 kb on - strand, within hsrA at 3937.665 kb on - strand, within hsrA at 3937.667 kb on + strand, within hsrA at 3937.725 kb on + strand, within hsrA at 3937.759 kb on + strand, within hsrA at 3937.789 kb on + strand, within hsrA at 3937.789 kb on + strand, within hsrA at 3937.792 kb on + strand, within hsrA at 3937.792 kb on + strand, within hsrA at 3937.792 kb on + strand, within hsrA at 3937.797 kb on - strand, within hsrA at 3937.797 kb on - strand, within hsrA at 3937.800 kb on - strand, within hsrA at 3937.800 kb on - strand, within hsrA at 3937.800 kb on - strand, within hsrA at 3937.832 kb on - strand, within hsrA at 3937.995 kb on - strand, within hsrA at 3937.995 kb on - strand, within hsrA at 3938.052 kb on - strand, within hsrA at 3938.059 kb on + strand, within hsrA at 3938.059 kb on + strand, within hsrA at 3938.106 kb on + strand, within hsrA at 3938.106 kb on + strand, within hsrA at 3938.149 kb on - strand, within hsrA at 3938.154 kb on + strand, within hsrA at 3938.207 kb on - strand, within hsrA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2 remove 3,935,280 + -0.5 3,935,420 + rbsK b3752 0.11 +2.3 3,935,420 + rbsK b3752 0.11 +0.4 3,935,469 - rbsK b3752 0.16 +5.2 3,935,485 - rbsK b3752 0.18 +4.0 3,935,493 - rbsK b3752 0.19 +4.2 3,935,517 - rbsK b3752 0.22 +4.6 3,935,517 - rbsK b3752 0.22 +4.7 3,935,519 + rbsK b3752 0.22 +1.8 3,935,519 + rbsK b3752 0.22 +2.2 3,935,522 - rbsK b3752 0.22 +4.9 3,935,522 - rbsK b3752 0.22 +5.1 3,935,542 - rbsK b3752 0.24 +5.9 3,935,542 - rbsK b3752 0.24 +4.9 3,935,551 + rbsK b3752 0.25 +1.7 3,935,551 + rbsK b3752 0.25 +1.3 3,935,560 - rbsK b3752 0.26 +5.0 3,935,577 - rbsK b3752 0.28 +6.3 3,935,650 + rbsK b3752 0.36 +1.4 3,935,656 - rbsK b3752 0.36 +5.6 3,935,661 + rbsK b3752 0.37 +1.5 3,935,666 + rbsK b3752 0.38 +2.8 3,935,669 - rbsK b3752 0.38 +4.6 3,935,669 - rbsK b3752 0.38 +5.1 3,935,669 - rbsK b3752 0.38 +4.2 3,935,711 + rbsK b3752 0.42 +1.4 3,935,742 + rbsK b3752 0.46 +0.6 3,935,798 - rbsK b3752 0.52 +4.7 3,935,798 - rbsK b3752 0.52 +5.7 3,935,808 + rbsK b3752 0.53 +0.4 3,935,942 + rbsK b3752 0.67 +0.8 3,935,946 + rbsK b3752 0.68 +1.2 3,935,946 + rbsK b3752 0.68 -0.6 3,935,955 - rbsK b3752 0.69 +4.9 3,935,969 + rbsK b3752 0.70 -1.7 3,935,969 + rbsK b3752 0.70 +0.2 3,935,977 - rbsK b3752 0.71 +5.0 3,935,977 - rbsK b3752 0.71 +5.6 3,935,983 + rbsK b3752 0.72 +0.7 3,936,007 - rbsK b3752 0.74 +4.4 3,936,007 - rbsK b3752 0.74 +4.6 3,936,007 - rbsK b3752 0.74 +5.2 3,936,009 - rbsK b3752 0.74 +5.5 3,936,028 - rbsK b3752 0.76 +5.3 3,936,028 - rbsK b3752 0.76 +5.1 3,936,048 + rbsK b3752 0.79 -2.0 3,936,055 - rbsK b3752 0.79 +6.0 3,936,074 - rbsK b3752 0.81 +5.8 3,936,074 - rbsK b3752 0.81 +5.5 3,936,082 + rbsK b3752 0.82 -3.1 3,936,082 + rbsK b3752 0.82 -0.4 3,936,197 + +0.2 3,936,338 - +5.2 3,936,338 - +5.3 3,936,395 + rbsR b3753 0.15 +10.7 3,936,407 + rbsR b3753 0.16 +5.1 3,936,415 - rbsR b3753 0.17 +6.1 3,936,444 - rbsR b3753 0.20 +5.3 3,936,447 - rbsR b3753 0.20 +5.4 3,936,456 + rbsR b3753 0.21 +5.4 3,936,456 + rbsR b3753 0.21 +5.8 3,936,463 - rbsR b3753 0.21 +5.7 3,936,470 + rbsR b3753 0.22 +5.8 3,936,470 + rbsR b3753 0.22 +6.1 3,936,473 - rbsR b3753 0.22 +5.0 3,936,473 - rbsR b3753 0.22 +4.6 3,936,496 - rbsR b3753 0.25 +5.4 3,936,496 - rbsR b3753 0.25 +5.5 3,936,545 + rbsR b3753 0.30 +5.7 3,936,659 + rbsR b3753 0.41 +5.7 3,936,667 + rbsR b3753 0.42 +5.3 3,936,667 + rbsR b3753 0.42 +5.8 3,936,667 + rbsR b3753 0.42 +5.6 3,936,684 - rbsR b3753 0.44 +4.5 3,936,684 - rbsR b3753 0.44 +4.7 3,936,686 + rbsR b3753 0.44 +5.7 3,936,723 - rbsR b3753 0.48 +5.8 3,936,726 - rbsR b3753 0.48 +4.4 3,936,763 - rbsR b3753 0.52 +4.8 3,936,769 + rbsR b3753 0.52 +4.7 3,936,794 + rbsR b3753 0.55 +5.7 3,936,842 - rbsR b3753 0.60 +5.6 3,936,868 + rbsR b3753 0.62 +5.8 3,936,868 + rbsR b3753 0.62 +5.9 3,936,868 + rbsR b3753 0.62 +5.7 3,936,875 + rbsR b3753 0.63 +5.7 3,936,876 - rbsR b3753 0.63 +5.7 3,936,876 - rbsR b3753 0.63 +5.4 3,936,878 + rbsR b3753 0.63 +5.4 3,936,878 + rbsR b3753 0.63 +5.6 3,936,943 + rbsR b3753 0.70 +5.4 3,936,953 - rbsR b3753 0.71 +5.2 3,936,953 - rbsR b3753 0.71 +5.2 3,936,989 + rbsR b3753 0.74 +6.2 3,936,989 + rbsR b3753 0.74 +5.5 3,936,990 + rbsR b3753 0.75 +5.9 3,936,992 + rbsR b3753 0.75 +5.6 3,937,039 + rbsR b3753 0.79 +5.7 3,937,055 - rbsR b3753 0.81 +5.9 3,937,101 + rbsR b3753 0.86 +5.7 3,937,124 - rbsR b3753 0.88 +5.7 3,937,124 - rbsR b3753 0.88 +5.4 3,937,130 - rbsR b3753 0.89 +5.9 3,937,137 + rbsR b3753 0.89 +5.7 3,937,174 + +5.8 3,937,174 + +5.7 3,937,177 - +5.5 3,937,184 - +4.6 3,937,186 + +5.8 3,937,186 + +5.5 3,937,201 + +4.8 3,937,205 + +5.6 3,937,244 - -1.1 3,937,244 - -1.6 3,937,312 - +0.5 3,937,318 - -1.2 3,937,318 - -0.5 3,937,342 - +1.0 3,937,365 + hsrA b3754 0.11 +0.9 3,937,373 + hsrA b3754 0.12 +0.3 3,937,406 + hsrA b3754 0.14 +0.1 3,937,418 + hsrA b3754 0.15 -0.9 3,937,458 + hsrA b3754 0.18 +0.4 3,937,458 - hsrA b3754 0.18 +0.3 3,937,467 + hsrA b3754 0.18 -0.4 3,937,470 - hsrA b3754 0.18 -3.4 3,937,470 - hsrA b3754 0.18 +0.1 3,937,498 + hsrA b3754 0.20 -1.1 3,937,506 + hsrA b3754 0.21 +0.8 3,937,506 - hsrA b3754 0.21 -2.0 3,937,506 - hsrA b3754 0.21 -0.0 3,937,511 - hsrA b3754 0.21 +0.2 3,937,523 - hsrA b3754 0.22 -2.2 3,937,533 - hsrA b3754 0.23 +0.3 3,937,533 - hsrA b3754 0.23 +0.7 3,937,541 + hsrA b3754 0.23 +0.0 3,937,549 - hsrA b3754 0.24 +0.3 3,937,570 - hsrA b3754 0.25 -3.5 3,937,570 - hsrA b3754 0.25 +0.0 3,937,579 - hsrA b3754 0.26 +1.3 3,937,579 - hsrA b3754 0.26 +1.0 3,937,592 - hsrA b3754 0.27 -1.6 3,937,592 - hsrA b3754 0.27 +0.4 3,937,596 + hsrA b3754 0.27 +1.7 3,937,630 + hsrA b3754 0.30 +1.2 3,937,647 - hsrA b3754 0.31 -0.7 3,937,647 - hsrA b3754 0.31 +0.2 3,937,651 + hsrA b3754 0.31 -0.8 3,937,654 - hsrA b3754 0.31 +0.9 3,937,665 - hsrA b3754 0.32 +0.1 3,937,667 + hsrA b3754 0.32 +0.9 3,937,725 + hsrA b3754 0.36 -0.4 3,937,759 + hsrA b3754 0.39 +1.3 3,937,789 + hsrA b3754 0.41 -0.4 3,937,789 + hsrA b3754 0.41 -0.7 3,937,792 + hsrA b3754 0.41 +0.9 3,937,792 + hsrA b3754 0.41 +0.3 3,937,792 + hsrA b3754 0.41 -2.3 3,937,797 - hsrA b3754 0.41 -4.1 3,937,797 - hsrA b3754 0.41 -0.2 3,937,800 - hsrA b3754 0.41 +1.0 3,937,800 - hsrA b3754 0.41 -3.9 3,937,800 - hsrA b3754 0.41 -0.0 3,937,832 - hsrA b3754 0.44 +0.9 3,937,995 - hsrA b3754 0.55 +1.5 3,937,995 - hsrA b3754 0.55 +0.1 3,938,052 - hsrA b3754 0.59 -1.4 3,938,059 + hsrA b3754 0.60 -0.0 3,938,059 + hsrA b3754 0.60 +0.0 3,938,106 + hsrA b3754 0.63 -0.1 3,938,106 + hsrA b3754 0.63 +0.3 3,938,149 - hsrA b3754 0.66 +0.6 3,938,154 + hsrA b3754 0.66 -1.1 3,938,207 - hsrA b3754 0.70 -0.3
Or see this region's nucleotide sequence