Strain Fitness in Escherichia coli BW25113 around b3649

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyicF and gmk are separated by 257 nucleotidesgmk and rpoZ are separated by 54 nucleotidesrpoZ and spoT are separated by 18 nucleotides b3647: yicF - putative enzyme (VIMSS), at 3,817,511 to 3,819,193 yicF b3648: gmk - guanylate kinase (NCBI), at 3,819,451 to 3,820,074 gmk b3649: rpoZ - DNA-directed RNA polymerase subunit omega (NCBI), at 3,820,129 to 3,820,404 rpoZ b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531 spoT Position (kb) 3820 3821Strain fitness (log2 ratio) -2 -1 0 1 2at 3819.130 kb on - strandat 3819.173 kb on - strandat 3819.191 kb on + strandat 3819.197 kb on - strandat 3819.197 kb on - strandat 3819.212 kb on - strandat 3819.234 kb on + strandat 3819.252 kb on - strandat 3819.252 kb on - strandat 3819.303 kb on - strandat 3819.394 kb on - strandat 3819.394 kb on - strandat 3820.087 kb on - strandat 3820.111 kb on - strandat 3820.144 kb on + strandat 3820.144 kb on + strandat 3820.151 kb on + strandat 3820.205 kb on + strand, within rpoZat 3820.205 kb on + strand, within rpoZat 3820.210 kb on + strand, within rpoZat 3820.297 kb on + strand, within rpoZat 3820.297 kb on + strand, within rpoZat 3820.340 kb on + strand, within rpoZat 3820.341 kb on - strand, within rpoZat 3820.365 kb on + strand, within rpoZat 3820.420 kb on + strandat 3820.420 kb on + strandat 3820.448 kb on - strandat 3820.452 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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3,819,130 - -2.3
3,819,173 - -0.3
3,819,191 + +0.3
3,819,197 - +0.1
3,819,197 - +0.0
3,819,212 - -0.7
3,819,234 + +0.2
3,819,252 - +0.5
3,819,252 - -0.4
3,819,303 - -0.3
3,819,394 - -0.3
3,819,394 - -0.5
3,820,087 - +1.5
3,820,111 - +1.5
3,820,144 + +2.2
3,820,144 + +2.2
3,820,151 + -0.4
3,820,205 + rpoZ b3649 0.28 -0.6
3,820,205 + rpoZ b3649 0.28 +2.3
3,820,210 + rpoZ b3649 0.29 +1.6
3,820,297 + rpoZ b3649 0.61 +1.7
3,820,297 + rpoZ b3649 0.61 +1.3
3,820,340 + rpoZ b3649 0.76 -2.6
3,820,341 - rpoZ b3649 0.77 -2.1
3,820,365 + rpoZ b3649 0.86 -0.2
3,820,420 + +0.2
3,820,420 + -0.1
3,820,448 - +0.2
3,820,452 + +1.4

Or see this region's nucleotide sequence