Strain Fitness in Escherichia coli BW25113 around b2660

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2659 and ygaF are separated by 19 nucleotidesygaF and gabD are separated by 22 nucleotides b2659: b2659 - orf, hypothetical protein (VIMSS), at 2,787,007 to 2,787,984 b2659 b2660: ygaF - orf, hypothetical protein (VIMSS), at 2,788,004 to 2,789,272 ygaF b2661: gabD - succinate-semialdehyde dehydrogenase I, NADP-dependent (NCBI), at 2,789,295 to 2,790,743 gabD Position (kb) 2788 2789 2790Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2787.071 kb on + strandat 2787.166 kb on + strand, within b2659at 2787.166 kb on + strand, within b2659at 2787.265 kb on + strand, within b2659at 2787.737 kb on - strand, within b2659at 2788.053 kb on + strandat 2788.138 kb on + strand, within ygaFat 2788.165 kb on + strand, within ygaFat 2788.227 kb on - strand, within ygaFat 2788.250 kb on + strand, within ygaFat 2788.269 kb on - strand, within ygaFat 2788.322 kb on - strand, within ygaFat 2788.324 kb on - strand, within ygaFat 2788.324 kb on - strand, within ygaFat 2788.349 kb on - strand, within ygaFat 2788.551 kb on + strand, within ygaFat 2788.551 kb on + strand, within ygaFat 2788.665 kb on - strand, within ygaFat 2788.753 kb on - strand, within ygaFat 2788.753 kb on - strand, within ygaFat 2788.861 kb on + strand, within ygaFat 2788.861 kb on + strand, within ygaFat 2788.931 kb on - strand, within ygaFat 2789.116 kb on - strand, within ygaFat 2789.211 kb on - strandat 2789.230 kb on + strandat 2789.230 kb on + strandat 2789.254 kb on - strandat 2789.254 kb on - strandat 2789.309 kb on - strandat 2789.344 kb on - strandat 2789.436 kb on + strandat 2789.699 kb on + strand, within gabDat 2789.739 kb on - strand, within gabDat 2789.739 kb on - strand, within gabDat 2789.765 kb on - strand, within gabDat 2789.952 kb on - strand, within gabDat 2790.018 kb on + strand, within gabDat 2790.018 kb on + strand, within gabDat 2790.034 kb on - strand, within gabDat 2790.099 kb on + strand, within gabDat 2790.099 kb on + strand, within gabDat 2790.123 kb on + strand, within gabDat 2790.123 kb on + strand, within gabD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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2,787,071 + +1.4
2,787,166 + b2659 b2659 0.16 +0.5
2,787,166 + b2659 b2659 0.16 +0.9
2,787,265 + b2659 b2659 0.26 -0.3
2,787,737 - b2659 b2659 0.75 +0.4
2,788,053 + +0.7
2,788,138 + ygaF b2660 0.11 +1.4
2,788,165 + ygaF b2660 0.13 +0.2
2,788,227 - ygaF b2660 0.18 -1.1
2,788,250 + ygaF b2660 0.19 +1.0
2,788,269 - ygaF b2660 0.21 +0.1
2,788,322 - ygaF b2660 0.25 -1.6
2,788,324 - ygaF b2660 0.25 -2.9
2,788,324 - ygaF b2660 0.25 +0.6
2,788,349 - ygaF b2660 0.27 +0.9
2,788,551 + ygaF b2660 0.43 -0.2
2,788,551 + ygaF b2660 0.43 -0.2
2,788,665 - ygaF b2660 0.52 +0.6
2,788,753 - ygaF b2660 0.59 -0.4
2,788,753 - ygaF b2660 0.59 +0.4
2,788,861 + ygaF b2660 0.68 +4.6
2,788,861 + ygaF b2660 0.68 +1.1
2,788,931 - ygaF b2660 0.73 +0.3
2,789,116 - ygaF b2660 0.88 +0.0
2,789,211 - -0.9
2,789,230 + +2.6
2,789,230 + +2.6
2,789,254 - +0.5
2,789,254 - -1.5
2,789,309 - -0.6
2,789,344 - +0.0
2,789,436 + +1.3
2,789,699 + gabD b2661 0.28 +0.2
2,789,739 - gabD b2661 0.31 -0.5
2,789,739 - gabD b2661 0.31 -2.6
2,789,765 - gabD b2661 0.32 +0.1
2,789,952 - gabD b2661 0.45 -1.1
2,790,018 + gabD b2661 0.50 +0.6
2,790,018 + gabD b2661 0.50 +0.8
2,790,034 - gabD b2661 0.51 +0.2
2,790,099 + gabD b2661 0.55 +0.4
2,790,099 + gabD b2661 0.55 +0.0
2,790,123 + gabD b2661 0.57 -0.0
2,790,123 + gabD b2661 0.57 +0.9

Or see this region's nucleotide sequence