Experiment: Ying_Others16 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt smpA and b2618 are separated by 61 nucleotides b2618 and yfjG overlap by 11 nucleotides yfjG and smpB are separated by 131 nucleotides smpB and ssrA are separated by 214 nucleotides ssrA and intA are separated by 203 nucleotides
b2617: smpA - small membrane protein A (VIMSS), at 2,751,627 to 2,751,968
smpA
b2618: b2618 - orf, hypothetical protein (VIMSS), at 2,752,030 to 2,752,320
b2618
b2619: yfjG - hypothetical protein (NCBI), at 2,752,310 to 2,752,786
yfjG
b2620: smpB - SsrA-binding protein (NCBI), at 2,752,918 to 2,753,400
smpB
b2621: ssrA - 10Sa RNA SsrA (NCBI), at 2,753,615 to 2,753,977
ssrA
b2622: intA - CP4-57 prophage; integrase (NCBI), at 2,754,181 to 2,755,422
intA
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2751.941 kb on + strand at 2752.081 kb on + strand, within b2618 at 2752.081 kb on + strand, within b2618 at 2752.092 kb on + strand, within b2618 at 2752.118 kb on - strand, within b2618 at 2752.150 kb on + strand, within b2618 at 2752.822 kb on + strand at 2752.851 kb on - strand at 2752.936 kb on + strand at 2753.096 kb on + strand, within smpB at 2753.223 kb on - strand, within smpB at 2753.223 kb on - strand, within smpB at 2753.403 kb on + strand at 2753.530 kb on + strand at 2754.108 kb on + strand at 2754.230 kb on + strand at 2754.297 kb on + strand at 2754.297 kb on + strand at 2754.367 kb on - strand, within intA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2 remove 2,751,941 + +0.3 2,752,081 + b2618 b2618 0.18 +0.4 2,752,081 + b2618 b2618 0.18 +1.0 2,752,092 + b2618 b2618 0.21 +0.2 2,752,118 - b2618 b2618 0.30 +1.0 2,752,150 + b2618 b2618 0.41 +0.5 2,752,822 + -0.5 2,752,851 - +0.8 2,752,936 + +2.5 2,753,096 + smpB b2620 0.37 +1.8 2,753,223 - smpB b2620 0.63 -1.7 2,753,223 - smpB b2620 0.63 +1.2 2,753,403 + +0.5 2,753,530 + +1.6 2,754,108 + +0.0 2,754,230 + -1.0 2,754,297 + -0.3 2,754,297 + -1.4 2,754,367 - intA b2622 0.15 -2.7
Or see this region's nucleotide sequence