Strain Fitness in Escherichia coli BW25113 around b1279

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgpB and yciS are separated by 148 nucleotidesyciS and yciM are separated by 6 nucleotides b1278: pgpB - phosphatidylglycerophosphatase B (NCBI), at 1,337,354 to 1,338,118 pgpB b1279: yciS - conserved inner membrane protein (NCBI), at 1,338,267 to 1,338,575 yciS b1280: yciM - hypothetical protein (NCBI), at 1,338,582 to 1,339,751 yciM Position (kb) 1338 1339Strain fitness (log2 ratio) -3 -2 -1 0 1at 1337.498 kb on + strand, within pgpBat 1337.503 kb on + strand, within pgpBat 1337.522 kb on - strand, within pgpBat 1338.069 kb on - strandat 1338.069 kb on - strandat 1338.322 kb on + strand, within yciS

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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1,337,498 + pgpB b1278 0.19 +0.1
1,337,503 + pgpB b1278 0.19 -0.3
1,337,522 - pgpB b1278 0.22 +1.7
1,338,069 - -2.7
1,338,069 - -1.4
1,338,322 + yciS b1279 0.18 -3.6

Or see this region's nucleotide sequence