Strain Fitness in Escherichia coli BW25113 around b2517

Experiment: Ying_Others16 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfgA and yfgB are separated by 284 nucleotidesyfgB and ndk are separated by 149 nucleotidesndk and pbpC are separated by 148 nucleotides b2516: yfgA - hypothetical protein (NCBI), at 2,639,853 to 2,640,866 yfgA b2517: yfgB - predicted enzyme (NCBI), at 2,641,151 to 2,642,305 yfgB b2518: ndk - nucleoside diphosphate kinase (NCBI), at 2,642,455 to 2,642,886 ndk b2519: pbpC - fused transglycosylase/transpeptidase (NCBI), at 2,643,035 to 2,645,347 pbpC Position (kb) 2641 2642 2643Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2640.158 kb on + strand, within yfgAat 2640.262 kb on - strand, within yfgAat 2640.262 kb on - strand, within yfgAat 2640.310 kb on + strand, within yfgAat 2640.315 kb on - strand, within yfgAat 2640.336 kb on + strand, within yfgAat 2640.336 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.347 kb on + strand, within yfgAat 2640.348 kb on + strand, within yfgAat 2640.384 kb on + strand, within yfgAat 2640.387 kb on - strand, within yfgAat 2640.442 kb on + strand, within yfgAat 2640.442 kb on + strand, within yfgAat 2640.953 kb on + strandat 2640.955 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.963 kb on + strandat 2640.971 kb on - strandat 2640.971 kb on - strandat 2641.026 kb on - strandat 2641.028 kb on + strandat 2641.038 kb on + strandat 2641.046 kb on - strandat 2641.046 kb on - strandat 2641.046 kb on - strandat 2641.173 kb on + strandat 2641.179 kb on + strandat 2641.181 kb on - strandat 2641.181 kb on - strandat 2641.187 kb on - strandat 2641.279 kb on + strand, within yfgBat 2641.279 kb on + strand, within yfgBat 2641.299 kb on - strand, within yfgBat 2641.344 kb on + strand, within yfgBat 2641.397 kb on - strand, within yfgBat 2641.453 kb on - strand, within yfgBat 2641.453 kb on - strand, within yfgBat 2641.671 kb on + strand, within yfgBat 2641.688 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.784 kb on - strand, within yfgBat 2641.819 kb on + strand, within yfgBat 2641.819 kb on + strand, within yfgBat 2641.923 kb on - strand, within yfgBat 2641.947 kb on + strand, within yfgBat 2641.947 kb on + strand, within yfgBat 2642.153 kb on - strand, within yfgBat 2642.153 kb on - strand, within yfgBat 2642.304 kb on - strandat 2642.392 kb on - strandat 2642.392 kb on - strandat 2642.509 kb on - strand, within ndkat 2642.509 kb on - strand, within ndkat 2642.590 kb on - strand, within ndkat 2642.592 kb on + strand, within ndkat 2642.684 kb on - strandat 2642.740 kb on - strand, within ndkat 2642.740 kb on - strand, within ndkat 2642.809 kb on + strand, within ndkat 2642.937 kb on + strandat 2643.036 kb on - strandat 2643.081 kb on + strandat 2643.131 kb on - strandat 2643.171 kb on - strandat 2643.235 kb on - strandat 2643.235 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2
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2,640,158 + yfgA b2516 0.30 +1.0
2,640,262 - yfgA b2516 0.40 +0.8
2,640,262 - yfgA b2516 0.40 +0.3
2,640,310 + yfgA b2516 0.45 +1.3
2,640,315 - yfgA b2516 0.46 +1.1
2,640,336 + yfgA b2516 0.48 +0.7
2,640,336 + yfgA b2516 0.48 +0.4
2,640,347 + yfgA b2516 0.49 +0.6
2,640,347 + yfgA b2516 0.49 +1.1
2,640,347 + yfgA b2516 0.49 +0.6
2,640,348 + yfgA b2516 0.49 +1.6
2,640,384 + yfgA b2516 0.52 +1.3
2,640,387 - yfgA b2516 0.53 -0.5
2,640,442 + yfgA b2516 0.58 +1.9
2,640,442 + yfgA b2516 0.58 -0.6
2,640,953 + +0.9
2,640,955 + +0.6
2,640,963 + +0.6
2,640,963 + -0.1
2,640,963 + +0.1
2,640,963 + -2.0
2,640,963 + -1.1
2,640,963 + +1.3
2,640,971 - -0.5
2,640,971 - +0.6
2,641,026 - -0.3
2,641,028 + +1.3
2,641,038 + -0.2
2,641,046 - -0.7
2,641,046 - -1.1
2,641,046 - -0.5
2,641,173 + +0.4
2,641,179 + -1.7
2,641,181 - +0.5
2,641,181 - -1.3
2,641,187 - +0.2
2,641,279 + yfgB b2517 0.11 +0.6
2,641,279 + yfgB b2517 0.11 +1.0
2,641,299 - yfgB b2517 0.13 +0.2
2,641,344 + yfgB b2517 0.17 +1.4
2,641,397 - yfgB b2517 0.21 +0.4
2,641,453 - yfgB b2517 0.26 -0.0
2,641,453 - yfgB b2517 0.26 +0.5
2,641,671 + yfgB b2517 0.45 +0.4
2,641,688 + yfgB b2517 0.46 -0.3
2,641,748 + yfgB b2517 0.52 +1.6
2,641,748 + yfgB b2517 0.52 +1.2
2,641,784 - yfgB b2517 0.55 +1.7
2,641,819 + yfgB b2517 0.58 +0.3
2,641,819 + yfgB b2517 0.58 +1.5
2,641,923 - yfgB b2517 0.67 +0.3
2,641,947 + yfgB b2517 0.69 -0.5
2,641,947 + yfgB b2517 0.69 +1.6
2,642,153 - yfgB b2517 0.87 +0.9
2,642,153 - yfgB b2517 0.87 +0.1
2,642,304 - +1.1
2,642,392 - +0.0
2,642,392 - +0.7
2,642,509 - ndk b2518 0.12 -0.5
2,642,509 - ndk b2518 0.12 -0.5
2,642,590 - ndk b2518 0.31 +0.7
2,642,592 + ndk b2518 0.32 +0.4
2,642,684 - -0.2
2,642,740 - ndk b2518 0.66 -0.3
2,642,740 - ndk b2518 0.66 -1.4
2,642,809 + ndk b2518 0.82 +1.4
2,642,937 + +0.3
2,643,036 - +1.2
2,643,081 + +0.5
2,643,131 - +0.3
2,643,171 - -0.7
2,643,235 - -0.8
2,643,235 - -2.9

Or see this region's nucleotide sequence