Experiment: Ying_Others16 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspA and pspB are separated by 53 nucleotides pspB and pspC overlap by 1 nucleotides pspC and pspD are separated by 8 nucleotides pspD and pspE are separated by 74 nucleotides pspE and ycjM are separated by 212 nucleotides
b1304: pspA - regulatory protein for phage-shock-protein operon (NCBI), at 1,366,103 to 1,366,771
pspA
b1305: pspB - phage shock protein B (NCBI), at 1,366,825 to 1,367,049
pspB
b1306: pspC - DNA-binding transcriptional activator (NCBI), at 1,367,049 to 1,367,408
pspC
b1307: pspD - peripheral inner membrane phage-shock protein (NCBI), at 1,367,417 to 1,367,638
pspD
b1308: pspE - thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI), at 1,367,713 to 1,368,027
pspE
b1309: ycjM - putative polysaccharide hydrolase (VIMSS), at 1,368,240 to 1,369,919
ycjM
Position (kb)
1367
1368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1366.212 kb on + strand, within pspA at 1366.230 kb on - strand, within pspA at 1366.244 kb on + strand, within pspA at 1366.373 kb on - strand, within pspA at 1366.560 kb on + strand, within pspA at 1366.628 kb on + strand, within pspA at 1366.818 kb on - strand at 1366.840 kb on - strand at 1366.896 kb on + strand, within pspB at 1366.931 kb on + strand, within pspB at 1366.945 kb on - strand, within pspB at 1367.086 kb on + strand, within pspC at 1367.105 kb on - strand, within pspC at 1367.191 kb on + strand, within pspC at 1367.191 kb on + strand, within pspC at 1367.384 kb on + strand at 1367.424 kb on + strand at 1367.424 kb on + strand at 1367.427 kb on + strand at 1367.591 kb on + strand, within pspD at 1367.631 kb on - strand at 1367.631 kb on - strand at 1367.795 kb on + strand, within pspE at 1367.795 kb on + strand, within pspE at 1367.813 kb on - strand, within pspE at 1367.842 kb on + strand, within pspE at 1367.852 kb on + strand, within pspE at 1367.860 kb on - strand, within pspE at 1367.932 kb on + strand, within pspE at 1367.941 kb on + strand, within pspE at 1367.990 kb on + strand, within pspE at 1367.991 kb on + strand, within pspE at 1367.991 kb on + strand at 1368.049 kb on - strand at 1368.065 kb on - strand at 1368.090 kb on + strand at 1368.156 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.323 kb on + strand at 1368.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2 remove 1,366,212 + pspA b1304 0.16 +0.5 1,366,230 - pspA b1304 0.19 -0.2 1,366,244 + pspA b1304 0.21 -0.8 1,366,373 - pspA b1304 0.40 +0.2 1,366,560 + pspA b1304 0.68 +0.8 1,366,628 + pspA b1304 0.78 +0.8 1,366,818 - +0.9 1,366,840 - -1.5 1,366,896 + pspB b1305 0.32 -0.2 1,366,931 + pspB b1305 0.47 +1.0 1,366,945 - pspB b1305 0.53 -0.6 1,367,086 + pspC b1306 0.10 +0.3 1,367,105 - pspC b1306 0.16 -0.3 1,367,191 + pspC b1306 0.39 +1.6 1,367,191 + pspC b1306 0.39 +1.7 1,367,384 + +1.1 1,367,424 + -0.1 1,367,424 + -1.1 1,367,427 + -0.6 1,367,591 + pspD b1307 0.78 -0.6 1,367,631 - -1.2 1,367,631 - -0.6 1,367,795 + pspE b1308 0.26 +0.8 1,367,795 + pspE b1308 0.26 +1.1 1,367,813 - pspE b1308 0.32 +1.1 1,367,842 + pspE b1308 0.41 -2.0 1,367,852 + pspE b1308 0.44 -2.3 1,367,860 - pspE b1308 0.47 -2.5 1,367,932 + pspE b1308 0.70 +0.2 1,367,941 + pspE b1308 0.72 +0.5 1,367,990 + pspE b1308 0.88 -3.4 1,367,991 + pspE b1308 0.88 -1.0 1,367,991 + -1.3 1,368,049 - +0.5 1,368,065 - +0.4 1,368,090 + +1.2 1,368,156 + -0.6 1,368,323 + +0.0 1,368,323 + -2.1 1,368,323 + +1.5 1,368,348 + +2.1
Or see this region's nucleotide sequence