Strain Fitness in Escherichia coli BW25113 around b0382

Experiment: Ying_Others16 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntddlA and yaiB are separated by 462 nucleotidesyaiB and phoA are separated by 100 nucleotides b0381: ddlA - D-alanylalanine synthetase (NCBI), at 399,053 to 400,147 ddlA b0382: yaiB - hypothetical protein (NCBI), at 400,610 to 400,870 yaiB b0383: phoA - alkaline phosphatase (VIMSS), at 400,971 to 402,386 phoA Position (kb) 400 401Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 399.659 kb on - strand, within ddlAat 399.707 kb on - strand, within ddlAat 399.727 kb on + strand, within ddlAat 399.727 kb on + strand, within ddlAat 399.787 kb on + strand, within ddlAat 399.824 kb on + strand, within ddlAat 399.916 kb on + strand, within ddlAat 399.933 kb on + strand, within ddlAat 399.948 kb on + strand, within ddlAat 399.948 kb on + strand, within ddlAat 400.092 kb on + strandat 400.092 kb on + strandat 400.093 kb on + strandat 400.093 kb on + strandat 400.168 kb on + strandat 400.178 kb on - strandat 400.240 kb on + strandat 400.240 kb on + strandat 400.251 kb on + strandat 400.258 kb on + strandat 400.263 kb on + strandat 400.264 kb on + strandat 400.282 kb on - strandat 400.288 kb on - strandat 400.373 kb on + strandat 400.559 kb on - strandat 400.561 kb on + strandat 400.638 kb on - strand, within yaiBat 400.667 kb on + strand, within yaiBat 400.675 kb on + strand, within yaiBat 400.684 kb on + strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.707 kb on + strand, within yaiBat 400.715 kb on - strand, within yaiBat 400.750 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.770 kb on - strand, within yaiBat 400.770 kb on - strand, within yaiBat 400.775 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.782 kb on + strand, within yaiBat 400.783 kb on + strand, within yaiBat 400.783 kb on + strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.812 kb on + strand, within yaiBat 400.812 kb on - strand, within yaiBat 400.815 kb on + strand, within yaiBat 400.815 kb on + strand, within yaiBat 400.816 kb on - strand, within yaiBat 400.823 kb on - strand, within yaiBat 400.828 kb on - strand, within yaiBat 400.835 kb on + strand, within yaiBat 400.865 kb on - strandat 400.898 kb on - strandat 400.899 kb on + strandat 400.901 kb on - strandat 400.901 kb on - strandat 400.915 kb on + strandat 400.915 kb on + strandat 400.982 kb on - strandat 400.992 kb on - strandat 400.998 kb on - strandat 401.004 kb on + strandat 401.006 kb on - strandat 401.006 kb on - strandat 401.015 kb on - strandat 401.015 kb on - strandat 401.020 kb on - strandat 401.078 kb on - strandat 401.086 kb on - strandat 401.088 kb on - strandat 401.088 kb on - strandat 401.091 kb on - strandat 401.091 kb on - strandat 401.105 kb on + strandat 401.113 kb on - strand, within phoAat 401.113 kb on - strand, within phoAat 401.113 kb on - strand, within phoAat 401.120 kb on + strand, within phoAat 401.123 kb on + strand, within phoAat 401.123 kb on + strand, within phoAat 401.125 kb on + strand, within phoAat 401.126 kb on - strand, within phoAat 401.133 kb on - strand, within phoAat 401.192 kb on - strand, within phoAat 401.199 kb on - strand, within phoAat 401.217 kb on - strand, within phoAat 401.271 kb on - strand, within phoAat 401.280 kb on + strand, within phoAat 401.282 kb on + strand, within phoAat 401.290 kb on - strand, within phoAat 401.332 kb on - strand, within phoAat 401.348 kb on + strand, within phoAat 401.348 kb on + strand, within phoAat 401.350 kb on - strand, within phoAat 401.350 kb on - strand, within phoAat 401.350 kb on - strand, within phoAat 401.363 kb on + strand, within phoAat 401.380 kb on + strand, within phoAat 401.486 kb on + strand, within phoAat 401.486 kb on + strand, within phoAat 401.494 kb on - strand, within phoAat 401.503 kb on + strand, within phoAat 401.616 kb on + strand, within phoAat 401.621 kb on + strand, within phoAat 401.653 kb on - strand, within phoAat 401.800 kb on + strand, within phoAat 401.800 kb on + strand, within phoAat 401.808 kb on - strand, within phoAat 401.808 kb on - strand, within phoAat 401.830 kb on - strand, within phoAat 401.839 kb on + strand, within phoA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2
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399,659 - ddlA b0381 0.55 -0.7
399,707 - ddlA b0381 0.60 +0.8
399,727 + ddlA b0381 0.62 +0.8
399,727 + ddlA b0381 0.62 +1.3
399,787 + ddlA b0381 0.67 -2.8
399,824 + ddlA b0381 0.70 -3.0
399,916 + ddlA b0381 0.79 +1.1
399,933 + ddlA b0381 0.80 -0.3
399,948 + ddlA b0381 0.82 +1.4
399,948 + ddlA b0381 0.82 +0.9
400,092 + -0.1
400,092 + +1.1
400,093 + +4.4
400,093 + +0.8
400,168 + -0.4
400,178 - +0.2
400,240 + -0.8
400,240 + +0.3
400,251 + -0.3
400,258 + +1.1
400,263 + -0.9
400,264 + +1.3
400,282 - -0.2
400,288 - -0.9
400,373 + -0.7
400,559 - +4.7
400,561 + -0.7
400,638 - yaiB b0382 0.11 +1.2
400,667 + yaiB b0382 0.22 +0.4
400,675 + yaiB b0382 0.25 +1.9
400,684 + yaiB b0382 0.28 +2.0
400,692 - yaiB b0382 0.31 +2.6
400,692 - yaiB b0382 0.31 -0.1
400,692 - yaiB b0382 0.31 +1.5
400,707 + yaiB b0382 0.37 +0.5
400,715 - yaiB b0382 0.40 +0.4
400,750 + yaiB b0382 0.54 +1.8
400,762 + yaiB b0382 0.58 +1.8
400,762 + yaiB b0382 0.58 +1.7
400,762 + yaiB b0382 0.58 +1.6
400,770 - yaiB b0382 0.61 +0.2
400,770 - yaiB b0382 0.61 +1.2
400,775 + yaiB b0382 0.63 +1.3
400,777 + yaiB b0382 0.64 +1.8
400,777 + yaiB b0382 0.64 +1.8
400,777 + yaiB b0382 0.64 +2.0
400,777 + yaiB b0382 0.64 -0.1
400,782 + yaiB b0382 0.66 +1.4
400,783 + yaiB b0382 0.66 +1.7
400,783 + yaiB b0382 0.66 +0.8
400,783 - yaiB b0382 0.66 +1.5
400,783 - yaiB b0382 0.66 +2.9
400,783 - yaiB b0382 0.66 +1.5
400,812 + yaiB b0382 0.77 +1.1
400,812 - yaiB b0382 0.77 +3.1
400,815 + yaiB b0382 0.79 +1.3
400,815 + yaiB b0382 0.79 +0.6
400,816 - yaiB b0382 0.79 +0.7
400,823 - yaiB b0382 0.82 +0.8
400,828 - yaiB b0382 0.84 +1.4
400,835 + yaiB b0382 0.86 +1.4
400,865 - +3.1
400,898 - +0.3
400,899 + +0.2
400,901 - +0.3
400,901 - +2.8
400,915 + +0.4
400,915 + -0.0
400,982 - +1.4
400,992 - -1.3
400,998 - +1.0
401,004 + -1.3
401,006 - +2.7
401,006 - -1.2
401,015 - -0.2
401,015 - -0.1
401,020 - -0.2
401,078 - +0.2
401,086 - +0.4
401,088 - +0.2
401,088 - -1.0
401,091 - +0.5
401,091 - -0.7
401,105 + -1.6
401,113 - phoA b0383 0.10 +0.5
401,113 - phoA b0383 0.10 -0.6
401,113 - phoA b0383 0.10 -0.8
401,120 + phoA b0383 0.11 +0.7
401,123 + phoA b0383 0.11 -0.1
401,123 + phoA b0383 0.11 +1.0
401,125 + phoA b0383 0.11 -0.3
401,126 - phoA b0383 0.11 -0.1
401,133 - phoA b0383 0.11 -0.7
401,192 - phoA b0383 0.16 +0.4
401,199 - phoA b0383 0.16 -0.6
401,217 - phoA b0383 0.17 -0.1
401,271 - phoA b0383 0.21 +0.0
401,280 + phoA b0383 0.22 +1.2
401,282 + phoA b0383 0.22 +0.8
401,290 - phoA b0383 0.23 +0.4
401,332 - phoA b0383 0.25 -1.7
401,348 + phoA b0383 0.27 +0.2
401,348 + phoA b0383 0.27 +1.5
401,350 - phoA b0383 0.27 +0.2
401,350 - phoA b0383 0.27 -0.5
401,350 - phoA b0383 0.27 -0.1
401,363 + phoA b0383 0.28 -0.1
401,380 + phoA b0383 0.29 +0.2
401,486 + phoA b0383 0.36 +0.9
401,486 + phoA b0383 0.36 +1.5
401,494 - phoA b0383 0.37 -0.2
401,503 + phoA b0383 0.38 -0.5
401,616 + phoA b0383 0.46 +0.1
401,621 + phoA b0383 0.46 +1.0
401,653 - phoA b0383 0.48 +1.1
401,800 + phoA b0383 0.59 -0.4
401,800 + phoA b0383 0.59 +0.6
401,808 - phoA b0383 0.59 +0.4
401,808 - phoA b0383 0.59 -2.2
401,830 - phoA b0383 0.61 +1.2
401,839 + phoA b0383 0.61 +0.6

Or see this region's nucleotide sequence