Experiment: Ying_Others16 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinB and yafN are separated by 51 nucleotides yafN and yafO are separated by 2 nucleotides yafO and yafP are separated by 9 nucleotides yafP and ykfJ are separated by 317 nucleotides ykfJ and prfH are separated by 16 nucleotides
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
b0232: yafN - predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,005 to 252,298
yafN
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
b0235: ykfJ - hypothetical protein (RefSeq), at 253,479 to 253,685
ykfJ
b0236: prfH - peptide chain release factor 2 (NCBI), at 253,702 to 254,202
prfH
Position (kb) 252
253
254 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 251.803 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.818 kb on + strand, within dinB at 251.880 kb on + strand at 251.901 kb on + strand at 251.909 kb on - strand at 251.924 kb on + strand at 251.965 kb on - strand at 252.003 kb on - strand at 252.137 kb on - strand, within yafN at 252.155 kb on - strand, within yafN at 252.288 kb on + strand at 252.331 kb on + strand at 252.331 kb on + strand at 252.341 kb on - strand, within yafO at 252.346 kb on + strand, within yafO at 252.368 kb on - strand, within yafO at 252.368 kb on - strand, within yafO at 252.380 kb on + strand, within yafO at 252.381 kb on + strand, within yafO at 252.419 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.443 kb on - strand, within yafO at 252.448 kb on - strand, within yafO at 252.475 kb on + strand, within yafO at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.594 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP at 252.973 kb on + strand, within yafP at 252.974 kb on + strand, within yafP at 253.041 kb on + strand, within yafP at 253.077 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.102 kb on + strand, within yafP at 253.188 kb on + strand at 253.198 kb on - strand at 253.220 kb on + strand at 253.222 kb on + strand at 253.222 kb on - strand at 253.272 kb on - strand at 253.275 kb on + strand at 253.283 kb on - strand at 253.301 kb on + strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.376 kb on - strand at 253.381 kb on - strand at 253.383 kb on - strand at 253.386 kb on + strand at 253.387 kb on + strand at 253.394 kb on + strand at 253.394 kb on + strand at 253.396 kb on - strand at 253.422 kb on - strand at 253.422 kb on - strand at 253.423 kb on - strand at 253.423 kb on - strand at 253.427 kb on - strand at 253.428 kb on - strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.432 kb on - strand at 253.432 kb on - strand at 253.434 kb on + strand at 253.437 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.444 kb on - strand at 253.450 kb on - strand at 253.454 kb on - strand at 253.491 kb on + strand at 253.504 kb on - strand, within ykfJ at 253.504 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.523 kb on + strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.557 kb on - strand, within ykfJ at 253.569 kb on - strand, within ykfJ at 253.571 kb on - strand, within ykfJ at 253.593 kb on + strand, within ykfJ at 253.603 kb on + strand, within ykfJ at 253.608 kb on - strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.633 kb on - strand, within ykfJ at 253.637 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.681 kb on + strand at 253.727 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.734 kb on - strand at 253.734 kb on - strand at 253.735 kb on - strand at 253.735 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.747 kb on + strand at 253.765 kb on + strand, within prfH at 253.773 kb on + strand, within prfH at 253.805 kb on - strand, within prfH at 253.805 kb on - strand, within prfH at 253.838 kb on - strand, within prfH at 253.891 kb on - strand, within prfH
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2 remove 251,803 + dinB b0231 0.86 -0.0 251,803 + dinB b0231 0.86 +1.0 251,818 + dinB b0231 0.87 -0.8 251,880 + -0.3 251,901 + -1.1 251,909 - -1.0 251,924 + -0.2 251,965 - +3.2 252,003 - +0.6 252,137 - yafN b0232 0.45 +1.7 252,155 - yafN b0232 0.51 +2.5 252,288 + +0.6 252,331 + -2.0 252,331 + +1.3 252,341 - yafO b0233 0.10 -0.4 252,346 + yafO b0233 0.11 -2.6 252,368 - yafO b0233 0.17 +3.2 252,368 - yafO b0233 0.17 +3.2 252,380 + yafO b0233 0.20 -0.0 252,381 + yafO b0233 0.20 +0.5 252,419 + yafO b0233 0.30 -1.4 252,427 + yafO b0233 0.32 +1.6 252,427 + yafO b0233 0.32 +3.2 252,440 + yafO b0233 0.35 +1.9 252,440 + yafO b0233 0.35 +1.5 252,440 + yafO b0233 0.35 -2.0 252,440 + yafO b0233 0.35 -0.3 252,440 + yafO b0233 0.35 +1.4 252,443 - yafO b0233 0.36 +4.5 252,448 - yafO b0233 0.37 +3.2 252,475 + yafO b0233 0.44 +0.6 252,522 - yafO b0233 0.55 -0.8 252,522 - yafO b0233 0.55 -0.4 252,534 - yafO b0233 0.58 +1.3 252,538 + yafO b0233 0.59 -0.7 252,538 + yafO b0233 0.59 +1.8 252,563 + yafO b0233 0.66 -0.4 252,566 - yafO b0233 0.66 +2.2 252,574 - yafO b0233 0.68 +0.6 252,574 - yafO b0233 0.68 -2.4 252,592 - yafO b0233 0.73 +1.2 252,594 - yafO b0233 0.73 +3.2 252,597 + yafO b0233 0.74 -0.3 252,610 - yafO b0233 0.77 +2.2 252,614 + yafO b0233 0.78 -0.1 252,622 - yafO b0233 0.80 +3.2 252,628 + yafO b0233 0.82 +0.6 252,671 + +0.3 252,671 + +0.9 252,739 - +3.2 252,739 - +1.6 252,779 - yafP b0234 0.15 +2.2 252,781 + yafP b0234 0.16 +3.2 252,848 + yafP b0234 0.31 +0.8 252,849 + yafP b0234 0.31 +3.7 252,879 + yafP b0234 0.38 +1.6 252,896 + yafP b0234 0.41 +1.6 252,936 + yafP b0234 0.50 -1.5 252,973 + yafP b0234 0.58 +3.2 252,974 + yafP b0234 0.58 +3.2 253,041 + yafP b0234 0.73 -0.9 253,077 + yafP b0234 0.81 +0.8 253,087 + yafP b0234 0.83 +1.3 253,087 + yafP b0234 0.83 +2.7 253,087 + yafP b0234 0.83 +1.2 253,102 + yafP b0234 0.87 +2.2 253,188 + +2.2 253,198 - +1.2 253,220 + +0.6 253,222 + +3.1 253,222 - +3.2 253,272 - +1.6 253,275 + -0.2 253,283 - +2.2 253,301 + +1.6 253,350 - +0.3 253,350 - +0.2 253,350 - -0.2 253,350 - -0.2 253,376 - -0.7 253,381 - -0.6 253,383 - -0.8 253,386 + +3.2 253,387 + +3.2 253,394 + +3.2 253,394 + +2.2 253,396 - -0.5 253,422 - +0.3 253,422 - -0.7 253,423 - -0.7 253,423 - -2.2 253,427 - +0.6 253,428 - -0.9 253,430 + +0.3 253,430 + +0.1 253,430 + -1.9 253,430 + -0.1 253,432 - -2.4 253,432 - +0.6 253,434 + +0.6 253,437 - +0.2 253,438 - -0.2 253,438 - -0.2 253,438 - -1.3 253,438 - +0.2 253,444 - +0.6 253,450 - -0.4 253,454 - -0.3 253,491 + +1.6 253,504 - ykfJ b0235 0.12 +1.3 253,504 - ykfJ b0235 0.12 -0.8 253,520 - ykfJ b0235 0.20 -0.3 253,520 - ykfJ b0235 0.20 +0.1 253,520 - ykfJ b0235 0.20 -1.0 253,523 + ykfJ b0235 0.21 +1.6 253,533 - ykfJ b0235 0.26 +0.5 253,533 - ykfJ b0235 0.26 +0.3 253,533 - ykfJ b0235 0.26 +0.6 253,533 - ykfJ b0235 0.26 -2.7 253,535 + ykfJ b0235 0.27 +1.6 253,535 + ykfJ b0235 0.27 +2.2 253,543 - ykfJ b0235 0.31 -1.1 253,543 - ykfJ b0235 0.31 -0.8 253,543 - ykfJ b0235 0.31 +0.6 253,543 - ykfJ b0235 0.31 +0.3 253,543 - ykfJ b0235 0.31 +0.5 253,543 - ykfJ b0235 0.31 +1.1 253,543 - ykfJ b0235 0.31 +1.2 253,543 - ykfJ b0235 0.31 -0.4 253,557 - ykfJ b0235 0.38 +0.6 253,569 - ykfJ b0235 0.43 +0.9 253,571 - ykfJ b0235 0.44 +0.8 253,593 + ykfJ b0235 0.55 -0.3 253,603 + ykfJ b0235 0.60 +0.6 253,608 - ykfJ b0235 0.62 -1.9 253,617 + ykfJ b0235 0.67 +0.7 253,617 + ykfJ b0235 0.67 -0.9 253,617 + ykfJ b0235 0.67 -0.3 253,617 + ykfJ b0235 0.67 +0.2 253,625 - ykfJ b0235 0.71 +2.2 253,625 - ykfJ b0235 0.71 +0.6 253,625 - ykfJ b0235 0.71 +1.2 253,633 - ykfJ b0235 0.74 -0.8 253,637 - ykfJ b0235 0.76 +0.0 253,642 - ykfJ b0235 0.79 -0.6 253,642 - ykfJ b0235 0.79 -0.4 253,642 - ykfJ b0235 0.79 -0.5 253,681 + +0.4 253,727 + +0.2 253,728 + +0.8 253,728 + -0.1 253,728 + +0.4 253,734 - -2.9 253,734 - -1.7 253,735 - -0.7 253,735 - +0.2 253,736 - -2.2 253,736 - +1.6 253,736 - -0.5 253,747 + +0.3 253,765 + prfH b0236 0.13 -0.1 253,773 + prfH b0236 0.14 -0.5 253,805 - prfH b0236 0.21 +0.2 253,805 - prfH b0236 0.21 +1.0 253,838 - prfH b0236 0.27 +0.7 253,891 - prfH b0236 0.38 -1.6
Or see this region's nucleotide sequence