Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnL and phnK are separated by 110 nucleotides phnK and phnJ overlap by 4 nucleotides phnJ and phnI overlap by 8 nucleotides
b4096: phnL - carbon-phosphorus lyase complex subunit (NCBI), at 4,315,238 to 4,315,918
phnL
b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787
phnK
b4098: phnJ - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,784 to 4,317,629
phnJ
b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686
phnI
Position (kb)
4316
4317
4318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4316.095 kb on + strand at 4316.185 kb on + strand, within phnK at 4316.185 kb on + strand, within phnK at 4316.208 kb on - strand, within phnK at 4316.211 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.242 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.434 kb on + strand, within phnK at 4316.447 kb on - strand, within phnK at 4316.461 kb on - strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.564 kb on + strand, within phnK at 4316.654 kb on - strand, within phnK at 4316.891 kb on - strand, within phnJ at 4316.929 kb on + strand, within phnJ at 4316.929 kb on + strand, within phnJ at 4316.930 kb on - strand, within phnJ at 4316.932 kb on + strand, within phnJ at 4317.020 kb on + strand, within phnJ at 4317.028 kb on - strand, within phnJ at 4317.034 kb on - strand, within phnJ at 4317.071 kb on + strand, within phnJ at 4317.095 kb on + strand, within phnJ at 4317.114 kb on + strand, within phnJ at 4317.114 kb on + strand, within phnJ at 4317.154 kb on + strand, within phnJ at 4317.159 kb on + strand, within phnJ at 4317.168 kb on - strand, within phnJ at 4317.177 kb on + strand, within phnJ at 4317.177 kb on + strand, within phnJ at 4317.210 kb on - strand, within phnJ at 4317.215 kb on - strand, within phnJ at 4317.303 kb on + strand, within phnJ at 4317.340 kb on - strand, within phnJ at 4317.349 kb on + strand, within phnJ at 4317.394 kb on - strand, within phnJ at 4317.402 kb on - strand, within phnJ at 4317.428 kb on + strand, within phnJ at 4317.428 kb on + strand, within phnJ at 4317.481 kb on + strand, within phnJ at 4317.481 kb on + strand, within phnJ at 4317.620 kb on + strand at 4317.620 kb on + strand at 4317.631 kb on + strand at 4317.631 kb on + strand at 4317.634 kb on - strand at 4317.639 kb on - strand at 4317.658 kb on - strand at 4317.658 kb on - strand at 4317.686 kb on + strand at 4317.705 kb on + strand at 4317.708 kb on + strand at 4317.708 kb on + strand at 4317.853 kb on + strand, within phnI at 4317.854 kb on - strand, within phnI at 4317.980 kb on + strand, within phnI at 4318.053 kb on + strand, within phnI at 4318.053 kb on + strand, within phnI at 4318.081 kb on - strand, within phnI at 4318.081 kb on - strand, within phnI at 4318.138 kb on - strand, within phnI at 4318.217 kb on - strand, within phnI at 4318.321 kb on - strand, within phnI at 4318.326 kb on - strand, within phnI at 4318.392 kb on + strand, within phnI at 4318.400 kb on + strand, within phnI at 4318.489 kb on - strand, within phnI at 4318.549 kb on + strand, within phnI
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 4,316,095 + -1.1 4,316,185 + phnK b4097 0.21 -1.3 4,316,185 + phnK b4097 0.21 +0.7 4,316,208 - phnK b4097 0.24 -1.1 4,316,211 + phnK b4097 0.24 -3.0 4,316,242 + phnK b4097 0.28 +0.7 4,316,242 + phnK b4097 0.28 -0.0 4,316,434 + phnK b4097 0.53 +0.1 4,316,434 + phnK b4097 0.53 -0.6 4,316,447 - phnK b4097 0.55 -3.3 4,316,461 - phnK b4097 0.57 -1.6 4,316,564 + phnK b4097 0.70 -0.3 4,316,564 + phnK b4097 0.70 +0.0 4,316,654 - phnK b4097 0.82 -1.5 4,316,891 - phnJ b4098 0.13 -2.0 4,316,929 + phnJ b4098 0.17 -0.5 4,316,929 + phnJ b4098 0.17 -0.1 4,316,930 - phnJ b4098 0.17 -2.3 4,316,932 + phnJ b4098 0.17 +0.1 4,317,020 + phnJ b4098 0.28 -0.2 4,317,028 - phnJ b4098 0.29 -1.2 4,317,034 - phnJ b4098 0.30 -1.1 4,317,071 + phnJ b4098 0.34 -0.4 4,317,095 + phnJ b4098 0.37 -0.5 4,317,114 + phnJ b4098 0.39 -1.1 4,317,114 + phnJ b4098 0.39 -0.2 4,317,154 + phnJ b4098 0.44 -0.1 4,317,159 + phnJ b4098 0.44 -0.0 4,317,168 - phnJ b4098 0.45 -0.9 4,317,177 + phnJ b4098 0.46 -0.7 4,317,177 + phnJ b4098 0.46 -0.6 4,317,210 - phnJ b4098 0.50 -1.9 4,317,215 - phnJ b4098 0.51 +0.2 4,317,303 + phnJ b4098 0.61 +0.3 4,317,340 - phnJ b4098 0.66 -0.5 4,317,349 + phnJ b4098 0.67 +0.2 4,317,394 - phnJ b4098 0.72 -1.4 4,317,402 - phnJ b4098 0.73 -1.3 4,317,428 + phnJ b4098 0.76 -0.2 4,317,428 + phnJ b4098 0.76 -0.1 4,317,481 + phnJ b4098 0.82 -0.6 4,317,481 + phnJ b4098 0.82 +0.5 4,317,620 + +0.6 4,317,620 + -0.9 4,317,631 + -0.0 4,317,631 + -0.2 4,317,634 - -1.5 4,317,639 - -1.3 4,317,658 - -2.4 4,317,658 - -1.8 4,317,686 + -0.7 4,317,705 + +0.0 4,317,708 + -0.3 4,317,708 + +0.6 4,317,853 + phnI b4099 0.22 -0.9 4,317,854 - phnI b4099 0.22 +1.8 4,317,980 + phnI b4099 0.34 -0.1 4,318,053 + phnI b4099 0.40 +0.1 4,318,053 + phnI b4099 0.40 +0.0 4,318,081 - phnI b4099 0.43 -0.1 4,318,081 - phnI b4099 0.43 -1.2 4,318,138 - phnI b4099 0.48 -0.2 4,318,217 - phnI b4099 0.56 -1.0 4,318,321 - phnI b4099 0.66 -3.3 4,318,326 - phnI b4099 0.66 -0.4 4,318,392 + phnI b4099 0.72 -0.4 4,318,400 + phnI b4099 0.73 +0.2 4,318,489 - phnI b4099 0.81 -2.1 4,318,549 + phnI b4099 0.87 -0.0
Or see this region's nucleotide sequence