Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagD and nagC are separated by 47 nucleotides nagC and nagA are separated by 8 nucleotides
b0675: nagD - UMP phosphatase (NCBI), at 698,797 to 699,549
nagD
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
Position (kb) 699
700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 698.720 kb on + strand at 698.737 kb on + strand at 698.745 kb on - strand at 698.763 kb on - strand at 698.857 kb on + strand at 698.920 kb on - strand, within nagD at 698.950 kb on + strand, within nagD at 698.950 kb on + strand, within nagD at 698.951 kb on - strand, within nagD at 699.008 kb on + strand, within nagD at 699.024 kb on + strand, within nagD at 699.028 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.285 kb on - strand, within nagD at 699.291 kb on + strand, within nagD at 699.349 kb on - strand, within nagD at 699.349 kb on - strand, within nagD at 699.361 kb on + strand, within nagD at 699.405 kb on - strand, within nagD at 699.405 kb on - strand, within nagD at 699.453 kb on + strand, within nagD at 699.553 kb on - strand at 699.563 kb on - strand at 699.745 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.819 kb on + strand, within nagC at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC at 700.726 kb on - strand at 700.726 kb on - strand at 700.733 kb on + strand at 700.759 kb on + strand at 700.759 kb on + strand at 700.761 kb on + strand at 700.813 kb on - strand at 700.813 kb on - strand at 700.967 kb on - strand, within nagA at 700.977 kb on - strand, within nagA at 701.025 kb on - strand, within nagA at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 698,720 + +0.1 698,737 + +0.4 698,745 - -0.4 698,763 - -0.3 698,857 + +0.2 698,920 - nagD b0675 0.16 -0.1 698,950 + nagD b0675 0.20 +0.6 698,950 + nagD b0675 0.20 +0.8 698,951 - nagD b0675 0.20 -0.3 699,008 + nagD b0675 0.28 +0.3 699,024 + nagD b0675 0.30 +0.3 699,028 - nagD b0675 0.31 +0.1 699,263 - nagD b0675 0.62 +0.3 699,263 - nagD b0675 0.62 -0.8 699,285 - nagD b0675 0.65 -0.4 699,291 + nagD b0675 0.66 -0.1 699,349 - nagD b0675 0.73 +0.4 699,349 - nagD b0675 0.73 -0.0 699,361 + nagD b0675 0.75 +1.1 699,405 - nagD b0675 0.81 +0.0 699,405 - nagD b0675 0.81 -0.2 699,453 + nagD b0675 0.87 +0.7 699,553 - +0.4 699,563 - -0.1 699,745 - nagC b0676 0.12 -2.2 699,760 - nagC b0676 0.13 -0.8 699,760 - nagC b0676 0.13 -1.7 699,760 - nagC b0676 0.13 -1.5 699,819 + nagC b0676 0.18 -1.9 699,844 + nagC b0676 0.20 +0.4 699,854 + nagC b0676 0.21 -0.9 699,939 + nagC b0676 0.28 -2.5 699,966 - nagC b0676 0.30 -2.8 699,966 - nagC b0676 0.30 -0.6 699,968 + nagC b0676 0.30 -1.9 700,083 + nagC b0676 0.40 -2.7 700,083 + nagC b0676 0.40 -1.9 700,132 - nagC b0676 0.44 -0.0 700,132 - nagC b0676 0.44 -2.6 700,193 - nagC b0676 0.49 -2.4 700,193 - nagC b0676 0.49 -1.0 700,193 - nagC b0676 0.49 -1.9 700,206 - nagC b0676 0.50 -1.3 700,206 - nagC b0676 0.50 -0.5 700,269 + nagC b0676 0.55 -2.2 700,454 + nagC b0676 0.70 -1.2 700,486 + nagC b0676 0.73 -1.7 700,542 - nagC b0676 0.77 -2.0 700,726 - -2.1 700,726 - -1.4 700,733 + -1.2 700,759 + -2.2 700,759 + -0.4 700,761 + -1.0 700,813 - -2.3 700,813 - -1.0 700,967 - nagA b0677 0.12 -0.6 700,977 - nagA b0677 0.13 -0.5 701,025 - nagA b0677 0.17 -0.1 701,074 - nagA b0677 0.22 -1.3 701,191 + nagA b0677 0.32 +0.3 701,244 - nagA b0677 0.36 -1.6 701,309 - nagA b0677 0.42 -1.0 701,309 - nagA b0677 0.42 -0.6 701,321 + nagA b0677 0.43 +1.2 701,327 - nagA b0677 0.44 -0.4 701,347 - nagA b0677 0.45 -3.0 701,382 + nagA b0677 0.48 +0.1 701,417 + nagA b0677 0.51 -2.1 701,498 - nagA b0677 0.58 -1.7 701,531 - nagA b0677 0.61 -0.8 701,565 + nagA b0677 0.64 -0.8 701,565 + nagA b0677 0.64 -1.7 701,648 + nagA b0677 0.72 -0.4 701,692 + nagA b0677 0.75 -1.1 701,694 - nagA b0677 0.76 -0.5 701,755 + nagA b0677 0.81 +0.3 701,766 + nagA b0677 0.82 -0.5 701,805 - nagA b0677 0.85 -1.2
Or see this region's nucleotide sequence