Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entE and entB are separated by 13 nucleotides entB and entA overlap by 1 nucleotides entA and ybdB are separated by 2 nucleotides ybdB and cstA are separated by 180 nucleotides
b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903
entE
b0595: entB - isochorismatase (NCBI), at 626,917 to 627,774
entB
b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520
entA
b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936
ybdB
b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222
cstA
Position (kb) 627
628
629 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 626.851 kb on - strand at 626.896 kb on - strand at 626.908 kb on - strand at 626.908 kb on - strand at 626.937 kb on + strand at 626.937 kb on + strand at 627.113 kb on + strand, within entB at 627.113 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.367 kb on + strand, within entB at 627.437 kb on - strand, within entB at 627.437 kb on - strand, within entB at 627.550 kb on - strand, within entB at 627.610 kb on - strand, within entB at 627.619 kb on + strand, within entB at 627.619 kb on + strand, within entB at 627.629 kb on - strand, within entB at 627.706 kb on - strand at 627.751 kb on + strand at 627.825 kb on + strand at 627.950 kb on - strand, within entA at 628.176 kb on + strand, within entA at 628.200 kb on + strand, within entA at 628.208 kb on - strand, within entA at 628.219 kb on - strand, within entA at 628.257 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.297 kb on + strand, within entA at 628.297 kb on + strand, within entA at 628.319 kb on - strand, within entA at 628.387 kb on - strand, within entA at 628.463 kb on - strand at 628.463 kb on - strand at 628.601 kb on + strand, within ybdB at 628.609 kb on - strand, within ybdB at 628.618 kb on - strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.790 kb on - strand, within ybdB at 628.923 kb on - strand at 628.923 kb on - strand at 629.097 kb on - strand at 629.097 kb on - strand at 629.099 kb on + strand at 629.100 kb on - strand at 629.140 kb on + strand at 629.140 kb on + strand at 629.140 kb on + strand at 629.182 kb on + strand at 629.189 kb on + strand at 629.189 kb on + strand at 629.275 kb on + strand at 629.275 kb on + strand at 629.330 kb on - strand, within cstA at 629.330 kb on - strand, within cstA at 629.333 kb on + strand, within cstA at 629.333 kb on + strand, within cstA at 629.435 kb on - strand, within cstA at 629.512 kb on - strand, within cstA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 626,851 - +0.6 626,896 - +1.0 626,908 - -0.0 626,908 - +0.4 626,937 + -1.0 626,937 + -0.5 627,113 + entB b0595 0.23 -1.3 627,113 + entB b0595 0.23 -1.0 627,128 + entB b0595 0.25 -0.6 627,128 + entB b0595 0.25 -1.7 627,146 - entB b0595 0.27 -1.2 627,146 - entB b0595 0.27 -0.7 627,146 - entB b0595 0.27 -0.3 627,146 - entB b0595 0.27 -0.3 627,216 - entB b0595 0.35 -0.7 627,216 - entB b0595 0.35 -1.3 627,367 + entB b0595 0.52 -0.2 627,437 - entB b0595 0.61 -0.4 627,437 - entB b0595 0.61 -0.7 627,550 - entB b0595 0.74 -0.1 627,610 - entB b0595 0.81 +0.2 627,619 + entB b0595 0.82 -0.7 627,619 + entB b0595 0.82 -0.1 627,629 - entB b0595 0.83 -0.0 627,706 - +0.1 627,751 + +0.0 627,825 + -0.5 627,950 - entA b0596 0.24 -1.0 628,176 + entA b0596 0.54 -1.4 628,200 + entA b0596 0.57 -1.4 628,208 - entA b0596 0.58 +0.1 628,219 - entA b0596 0.60 -0.6 628,257 - entA b0596 0.65 -0.1 628,287 - entA b0596 0.69 -2.8 628,287 - entA b0596 0.69 -0.5 628,297 + entA b0596 0.70 -0.8 628,297 + entA b0596 0.70 -0.4 628,319 - entA b0596 0.73 -0.2 628,387 - entA b0596 0.82 -0.6 628,463 - +0.6 628,463 - -0.3 628,601 + ybdB b0597 0.19 -0.0 628,609 - ybdB b0597 0.21 +0.5 628,618 - ybdB b0597 0.23 -0.5 628,640 + ybdB b0597 0.28 -0.3 628,640 + ybdB b0597 0.28 -0.5 628,756 + ybdB b0597 0.56 -0.4 628,756 + ybdB b0597 0.56 -0.1 628,790 - ybdB b0597 0.64 +0.4 628,923 - +0.2 628,923 - -0.3 629,097 - -0.2 629,097 - +0.2 629,099 + +0.4 629,100 - +0.3 629,140 + -0.6 629,140 + +0.2 629,140 + +0.5 629,182 + -0.4 629,189 + +0.8 629,189 + +1.7 629,275 + -0.1 629,275 + +1.1 629,330 - cstA b0598 0.10 +0.3 629,330 - cstA b0598 0.10 -0.8 629,333 + cstA b0598 0.10 +0.1 629,333 + cstA b0598 0.10 +0.0 629,435 - cstA b0598 0.15 +0.2 629,512 - cstA b0598 0.19 +0.3
Or see this region's nucleotide sequence