Strain Fitness in Escherichia coli BW25113 around b0593

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfepB and entC are separated by 374 nucleotidesentC and entE are separated by 9 nucleotides b0592: fepB - iron-enterobactin transporter subunit (NCBI), at 622,777 to 623,733 fepB b0593: entC - isochorismate synthase (NCBI), at 624,108 to 625,283 entC b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903 entE Position (kb) 624 625 626Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 623.119 kb on + strand, within fepBat 623.140 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.199 kb on - strand, within fepBat 623.253 kb on - strand, within fepBat 623.341 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.426 kb on + strand, within fepBat 623.426 kb on + strand, within fepBat 623.458 kb on - strand, within fepBat 623.458 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.563 kb on + strand, within fepBat 623.576 kb on - strand, within fepBat 623.576 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.610 kb on + strand, within fepBat 623.672 kb on + strandat 623.708 kb on + strandat 623.806 kb on + strandat 623.822 kb on + strandat 623.822 kb on + strandat 623.830 kb on - strandat 623.832 kb on - strandat 623.841 kb on + strandat 623.857 kb on + strandat 623.865 kb on + strandat 623.869 kb on + strandat 623.869 kb on + strandat 623.879 kb on - strandat 623.907 kb on - strandat 623.907 kb on - strandat 624.008 kb on + strandat 624.130 kb on - strandat 624.130 kb on - strandat 624.138 kb on - strandat 624.138 kb on - strandat 624.259 kb on + strand, within entCat 624.428 kb on - strand, within entCat 624.428 kb on - strand, within entCat 624.490 kb on + strand, within entCat 624.547 kb on - strand, within entCat 624.580 kb on - strand, within entCat 624.691 kb on + strand, within entCat 624.691 kb on + strand, within entCat 624.699 kb on - strand, within entCat 624.699 kb on - strand, within entCat 624.699 kb on - strand, within entCat 624.831 kb on - strand, within entCat 624.875 kb on + strand, within entCat 624.947 kb on + strand, within entCat 624.947 kb on + strand, within entCat 624.984 kb on + strand, within entCat 624.984 kb on + strand, within entCat 625.127 kb on - strand, within entCat 625.365 kb on - strandat 625.365 kb on - strandat 625.401 kb on + strandat 625.401 kb on + strandat 625.433 kb on + strandat 625.593 kb on + strand, within entEat 625.604 kb on + strand, within entEat 625.604 kb on + strand, within entEat 625.617 kb on - strand, within entEat 625.688 kb on + strand, within entEat 625.744 kb on + strand, within entEat 625.744 kb on + strand, within entEat 625.746 kb on + strand, within entEat 625.900 kb on + strand, within entEat 625.958 kb on + strand, within entEat 625.964 kb on - strand, within entEat 625.964 kb on - strand, within entEat 625.971 kb on - strand, within entEat 626.073 kb on + strand, within entE

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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623,119 + fepB b0592 0.36 -2.0
623,140 - fepB b0592 0.38 -2.0
623,148 - fepB b0592 0.39 -3.0
623,148 - fepB b0592 0.39 -2.8
623,199 - fepB b0592 0.44 -3.0
623,253 - fepB b0592 0.50 -2.4
623,341 - fepB b0592 0.59 -2.0
623,405 - fepB b0592 0.66 -2.4
623,405 - fepB b0592 0.66 -1.2
623,426 + fepB b0592 0.68 -2.1
623,426 + fepB b0592 0.68 -2.3
623,458 - fepB b0592 0.71 -2.1
623,458 - fepB b0592 0.71 -2.8
623,467 - fepB b0592 0.72 -1.8
623,467 - fepB b0592 0.72 -2.1
623,470 - fepB b0592 0.72 -3.2
623,470 - fepB b0592 0.72 -2.8
623,563 + fepB b0592 0.82 -3.9
623,576 - fepB b0592 0.83 -3.6
623,576 - fepB b0592 0.83 -2.0
623,591 - fepB b0592 0.85 -4.1
623,591 - fepB b0592 0.85 -2.1
623,610 + fepB b0592 0.87 -3.1
623,672 + -2.0
623,708 + +0.2
623,806 + +0.1
623,822 + +0.3
623,822 + -0.4
623,830 - -0.1
623,832 - +0.5
623,841 + -0.0
623,857 + -1.3
623,865 + -0.2
623,869 + -0.2
623,869 + +0.6
623,879 - +0.5
623,907 - +0.1
623,907 - -1.0
624,008 + -0.0
624,130 - -0.6
624,130 - -0.4
624,138 - -0.6
624,138 - -1.0
624,259 + entC b0593 0.13 -0.4
624,428 - entC b0593 0.27 -0.4
624,428 - entC b0593 0.27 -1.4
624,490 + entC b0593 0.32 -0.5
624,547 - entC b0593 0.37 +0.0
624,580 - entC b0593 0.40 -0.3
624,691 + entC b0593 0.50 -0.8
624,691 + entC b0593 0.50 -0.8
624,699 - entC b0593 0.50 -0.5
624,699 - entC b0593 0.50 -0.3
624,699 - entC b0593 0.50 -0.2
624,831 - entC b0593 0.61 -0.5
624,875 + entC b0593 0.65 +0.1
624,947 + entC b0593 0.71 -0.9
624,947 + entC b0593 0.71 -0.1
624,984 + entC b0593 0.74 -1.1
624,984 + entC b0593 0.74 -0.6
625,127 - entC b0593 0.87 -0.1
625,365 - -0.1
625,365 - -0.9
625,401 + -0.7
625,401 + -1.3
625,433 + +0.1
625,593 + entE b0594 0.19 -0.7
625,604 + entE b0594 0.19 -0.6
625,604 + entE b0594 0.19 -0.5
625,617 - entE b0594 0.20 -0.7
625,688 + entE b0594 0.25 -0.7
625,744 + entE b0594 0.28 -0.7
625,744 + entE b0594 0.28 -0.3
625,746 + entE b0594 0.28 -0.2
625,900 + entE b0594 0.38 -0.5
625,958 + entE b0594 0.41 -2.8
625,964 - entE b0594 0.42 -0.6
625,964 - entE b0594 0.42 -0.8
625,971 - entE b0594 0.42 -0.1
626,073 + entE b0594 0.48 -0.2

Or see this region's nucleotide sequence