Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepB and entC are separated by 374 nucleotides entC and entE are separated by 9 nucleotides
b0592: fepB - iron-enterobactin transporter subunit (NCBI), at 622,777 to 623,733
fepB
b0593: entC - isochorismate synthase (NCBI), at 624,108 to 625,283
entC
b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903
entE
Position (kb)
624
625
626 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 623.119 kb on + strand, within fepB at 623.140 kb on - strand, within fepB at 623.148 kb on - strand, within fepB at 623.148 kb on - strand, within fepB at 623.199 kb on - strand, within fepB at 623.253 kb on - strand, within fepB at 623.341 kb on - strand, within fepB at 623.405 kb on - strand, within fepB at 623.405 kb on - strand, within fepB at 623.426 kb on + strand, within fepB at 623.426 kb on + strand, within fepB at 623.458 kb on - strand, within fepB at 623.458 kb on - strand, within fepB at 623.467 kb on - strand, within fepB at 623.467 kb on - strand, within fepB at 623.470 kb on - strand, within fepB at 623.470 kb on - strand, within fepB at 623.563 kb on + strand, within fepB at 623.576 kb on - strand, within fepB at 623.576 kb on - strand, within fepB at 623.591 kb on - strand, within fepB at 623.591 kb on - strand, within fepB at 623.610 kb on + strand, within fepB at 623.672 kb on + strand at 623.708 kb on + strand at 623.806 kb on + strand at 623.822 kb on + strand at 623.822 kb on + strand at 623.830 kb on - strand at 623.832 kb on - strand at 623.841 kb on + strand at 623.857 kb on + strand at 623.865 kb on + strand at 623.869 kb on + strand at 623.869 kb on + strand at 623.879 kb on - strand at 623.907 kb on - strand at 623.907 kb on - strand at 624.008 kb on + strand at 624.130 kb on - strand at 624.130 kb on - strand at 624.138 kb on - strand at 624.138 kb on - strand at 624.259 kb on + strand, within entC at 624.428 kb on - strand, within entC at 624.428 kb on - strand, within entC at 624.490 kb on + strand, within entC at 624.547 kb on - strand, within entC at 624.580 kb on - strand, within entC at 624.691 kb on + strand, within entC at 624.691 kb on + strand, within entC at 624.699 kb on - strand, within entC at 624.699 kb on - strand, within entC at 624.699 kb on - strand, within entC at 624.831 kb on - strand, within entC at 624.875 kb on + strand, within entC at 624.947 kb on + strand, within entC at 624.947 kb on + strand, within entC at 624.984 kb on + strand, within entC at 624.984 kb on + strand, within entC at 625.127 kb on - strand, within entC at 625.365 kb on - strand at 625.365 kb on - strand at 625.401 kb on + strand at 625.401 kb on + strand at 625.433 kb on + strand at 625.593 kb on + strand, within entE at 625.604 kb on + strand, within entE at 625.604 kb on + strand, within entE at 625.617 kb on - strand, within entE at 625.688 kb on + strand, within entE at 625.744 kb on + strand, within entE at 625.744 kb on + strand, within entE at 625.746 kb on + strand, within entE at 625.900 kb on + strand, within entE at 625.958 kb on + strand, within entE at 625.964 kb on - strand, within entE at 625.964 kb on - strand, within entE at 625.971 kb on - strand, within entE at 626.073 kb on + strand, within entE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 623,119 + fepB b0592 0.36 -2.0 623,140 - fepB b0592 0.38 -2.0 623,148 - fepB b0592 0.39 -3.0 623,148 - fepB b0592 0.39 -2.8 623,199 - fepB b0592 0.44 -3.0 623,253 - fepB b0592 0.50 -2.4 623,341 - fepB b0592 0.59 -2.0 623,405 - fepB b0592 0.66 -2.4 623,405 - fepB b0592 0.66 -1.2 623,426 + fepB b0592 0.68 -2.1 623,426 + fepB b0592 0.68 -2.3 623,458 - fepB b0592 0.71 -2.1 623,458 - fepB b0592 0.71 -2.8 623,467 - fepB b0592 0.72 -1.8 623,467 - fepB b0592 0.72 -2.1 623,470 - fepB b0592 0.72 -3.2 623,470 - fepB b0592 0.72 -2.8 623,563 + fepB b0592 0.82 -3.9 623,576 - fepB b0592 0.83 -3.6 623,576 - fepB b0592 0.83 -2.0 623,591 - fepB b0592 0.85 -4.1 623,591 - fepB b0592 0.85 -2.1 623,610 + fepB b0592 0.87 -3.1 623,672 + -2.0 623,708 + +0.2 623,806 + +0.1 623,822 + +0.3 623,822 + -0.4 623,830 - -0.1 623,832 - +0.5 623,841 + -0.0 623,857 + -1.3 623,865 + -0.2 623,869 + -0.2 623,869 + +0.6 623,879 - +0.5 623,907 - +0.1 623,907 - -1.0 624,008 + -0.0 624,130 - -0.6 624,130 - -0.4 624,138 - -0.6 624,138 - -1.0 624,259 + entC b0593 0.13 -0.4 624,428 - entC b0593 0.27 -0.4 624,428 - entC b0593 0.27 -1.4 624,490 + entC b0593 0.32 -0.5 624,547 - entC b0593 0.37 +0.0 624,580 - entC b0593 0.40 -0.3 624,691 + entC b0593 0.50 -0.8 624,691 + entC b0593 0.50 -0.8 624,699 - entC b0593 0.50 -0.5 624,699 - entC b0593 0.50 -0.3 624,699 - entC b0593 0.50 -0.2 624,831 - entC b0593 0.61 -0.5 624,875 + entC b0593 0.65 +0.1 624,947 + entC b0593 0.71 -0.9 624,947 + entC b0593 0.71 -0.1 624,984 + entC b0593 0.74 -1.1 624,984 + entC b0593 0.74 -0.6 625,127 - entC b0593 0.87 -0.1 625,365 - -0.1 625,365 - -0.9 625,401 + -0.7 625,401 + -1.3 625,433 + +0.1 625,593 + entE b0594 0.19 -0.7 625,604 + entE b0594 0.19 -0.6 625,604 + entE b0594 0.19 -0.5 625,617 - entE b0594 0.20 -0.7 625,688 + entE b0594 0.25 -0.7 625,744 + entE b0594 0.28 -0.7 625,744 + entE b0594 0.28 -0.3 625,746 + entE b0594 0.28 -0.2 625,900 + entE b0594 0.38 -0.5 625,958 + entE b0594 0.41 -2.8 625,964 - entE b0594 0.42 -0.6 625,964 - entE b0594 0.42 -0.8 625,971 - entE b0594 0.42 -0.1 626,073 + entE b0594 0.48 -0.2
Or see this region's nucleotide sequence