Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepE and fepC overlap by 4 nucleotides fepC and fepG overlap by 4 nucleotides fepG and fepD overlap by 4 nucleotides
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422
fepC
b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411
fepG
b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412
fepD
Position (kb) 619
620
621 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 618.457 kb on + strand, within fepE at 618.484 kb on + strand, within fepE at 618.498 kb on + strand at 618.500 kb on - strand at 618.500 kb on - strand at 618.520 kb on - strand at 618.545 kb on - strand at 618.561 kb on - strand at 618.561 kb on - strand at 618.598 kb on - strand at 618.721 kb on + strand, within fepC at 618.725 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.876 kb on - strand, within fepC at 618.905 kb on - strand, within fepC at 618.927 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.970 kb on - strand, within fepC at 619.003 kb on + strand, within fepC at 619.038 kb on + strand, within fepC at 619.066 kb on - strand, within fepC at 619.131 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.261 kb on + strand, within fepC at 619.375 kb on + strand at 619.568 kb on - strand, within fepG at 619.568 kb on - strand, within fepG at 619.680 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.808 kb on + strand, within fepG at 619.812 kb on + strand, within fepG at 619.827 kb on - strand, within fepG at 619.870 kb on - strand, within fepG at 619.872 kb on + strand, within fepG at 619.948 kb on - strand, within fepG at 620.042 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.203 kb on - strand, within fepG at 620.205 kb on + strand, within fepG at 620.205 kb on + strand, within fepG at 620.263 kb on - strand, within fepG at 620.336 kb on - strand at 620.392 kb on - strand at 620.482 kb on - strand at 620.514 kb on - strand, within fepD at 620.634 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.653 kb on - strand, within fepD at 620.664 kb on - strand, within fepD at 620.672 kb on - strand, within fepD at 620.728 kb on - strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.749 kb on + strand, within fepD at 620.780 kb on - strand, within fepD at 620.808 kb on + strand, within fepD at 620.808 kb on + strand, within fepD at 620.892 kb on + strand, within fepD at 620.900 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.964 kb on - strand, within fepD at 620.970 kb on + strand, within fepD at 620.970 kb on + strand, within fepD at 621.018 kb on - strand, within fepD at 621.228 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.295 kb on - strand, within fepD at 621.331 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 618,457 + fepE b0587 0.86 -0.1 618,484 + fepE b0587 0.89 +0.2 618,498 + -0.2 618,500 - +0.3 618,500 - -1.3 618,520 - +0.4 618,545 - +0.2 618,561 - +0.3 618,561 - +0.3 618,598 - -0.4 618,721 + fepC b0588 0.14 -1.8 618,725 - fepC b0588 0.14 -2.3 618,815 - fepC b0588 0.25 -1.8 618,815 - fepC b0588 0.25 -3.2 618,876 - fepC b0588 0.33 -2.6 618,905 - fepC b0588 0.37 -2.2 618,927 - fepC b0588 0.39 -3.7 618,935 - fepC b0588 0.40 -3.4 618,935 - fepC b0588 0.40 -3.3 618,970 - fepC b0588 0.44 -2.4 619,003 + fepC b0588 0.49 -1.1 619,038 + fepC b0588 0.53 -3.5 619,066 - fepC b0588 0.56 -3.1 619,131 - fepC b0588 0.64 -4.0 619,259 - fepC b0588 0.80 -1.3 619,259 - fepC b0588 0.80 -2.1 619,261 + fepC b0588 0.80 -2.2 619,375 + -1.8 619,568 - fepG b0589 0.15 -2.1 619,568 - fepG b0589 0.15 -1.4 619,680 - fepG b0589 0.26 -3.2 619,685 - fepG b0589 0.27 -1.9 619,685 - fepG b0589 0.27 -2.5 619,750 - fepG b0589 0.33 -3.1 619,750 - fepG b0589 0.33 -3.0 619,808 + fepG b0589 0.39 -3.2 619,812 + fepG b0589 0.40 -3.4 619,827 - fepG b0589 0.41 -2.5 619,870 - fepG b0589 0.45 -2.6 619,872 + fepG b0589 0.46 -1.5 619,948 - fepG b0589 0.53 -2.7 620,042 - fepG b0589 0.63 -3.7 620,050 - fepG b0589 0.64 -1.8 620,050 - fepG b0589 0.64 -3.3 620,203 - fepG b0589 0.79 -2.8 620,205 + fepG b0589 0.79 -3.2 620,205 + fepG b0589 0.79 -2.9 620,263 - fepG b0589 0.85 -2.8 620,336 - -2.9 620,392 - -2.5 620,482 - -3.5 620,514 - fepD b0590 0.11 -1.9 620,634 - fepD b0590 0.22 -3.1 620,653 - fepD b0590 0.24 -2.8 620,653 - fepD b0590 0.24 -1.8 620,664 - fepD b0590 0.25 -0.9 620,672 - fepD b0590 0.26 -1.8 620,728 - fepD b0590 0.32 -3.8 620,749 + fepD b0590 0.34 -3.1 620,749 + fepD b0590 0.34 -2.8 620,749 + fepD b0590 0.34 -1.1 620,780 - fepD b0590 0.37 -3.1 620,808 + fepD b0590 0.40 -3.5 620,808 + fepD b0590 0.40 -3.0 620,892 + fepD b0590 0.48 -1.8 620,900 - fepD b0590 0.49 -2.6 620,964 - fepD b0590 0.55 -3.2 620,964 - fepD b0590 0.55 -2.6 620,970 + fepD b0590 0.56 -2.4 620,970 + fepD b0590 0.56 -2.6 621,018 - fepD b0590 0.61 -2.6 621,228 - fepD b0590 0.82 -2.1 621,295 - fepD b0590 0.88 -2.1 621,295 - fepD b0590 0.88 -2.3 621,331 + -2.7
Or see this region's nucleotide sequence