Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fes and ybdZ are separated by 2 nucleotides ybdZ and entF overlap by 4 nucleotides entF and fepE are separated by 215 nucleotides
b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162
fes
b4511: ybdZ - hypothetical protein (NCBI), at 613,165 to 613,383
ybdZ
b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261
entF
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
Position (kb) 613
614
615
616
617
618 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 612.424 kb on + strand, within fes at 612.424 kb on + strand, within fes at 612.629 kb on - strand, within fes at 612.629 kb on - strand, within fes at 612.676 kb on - strand, within fes at 612.835 kb on + strand, within fes at 612.840 kb on + strand, within fes at 612.843 kb on - strand, within fes at 612.843 kb on - strand, within fes at 612.868 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.871 kb on + strand, within fes at 612.883 kb on + strand, within fes at 612.960 kb on - strand, within fes at 613.018 kb on + strand, within fes at 613.018 kb on + strand, within fes at 613.181 kb on - strand at 613.181 kb on - strand at 613.203 kb on + strand, within ybdZ at 613.270 kb on + strand, within ybdZ at 613.340 kb on + strand, within ybdZ at 613.396 kb on - strand at 613.455 kb on + strand at 613.455 kb on + strand at 613.523 kb on + strand at 613.523 kb on + strand at 613.549 kb on - strand at 613.549 kb on - strand at 613.589 kb on + strand at 613.643 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.671 kb on - strand at 613.671 kb on - strand at 613.870 kb on - strand, within entF at 613.923 kb on + strand, within entF at 613.923 kb on + strand, within entF at 613.927 kb on - strand, within entF at 613.974 kb on + strand, within entF at 614.028 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.181 kb on - strand, within entF at 614.216 kb on + strand, within entF at 614.216 kb on + strand, within entF at 614.421 kb on + strand, within entF at 614.517 kb on - strand, within entF at 614.517 kb on - strand, within entF at 614.517 kb on - strand, within entF at 614.631 kb on + strand, within entF at 614.665 kb on - strand, within entF at 614.665 kb on - strand, within entF at 614.711 kb on + strand, within entF at 614.716 kb on + strand, within entF at 614.716 kb on + strand, within entF at 614.886 kb on + strand, within entF at 614.910 kb on + strand, within entF at 614.969 kb on + strand, within entF at 614.977 kb on - strand, within entF at 614.993 kb on - strand, within entF at 615.069 kb on - strand, within entF at 615.121 kb on + strand, within entF at 615.126 kb on + strand, within entF at 615.369 kb on - strand, within entF at 615.593 kb on - strand, within entF at 615.600 kb on + strand, within entF at 615.600 kb on + strand, within entF at 615.688 kb on - strand, within entF at 615.726 kb on + strand, within entF at 615.879 kb on - strand, within entF at 615.881 kb on - strand, within entF at 616.125 kb on + strand, within entF at 616.185 kb on - strand, within entF at 616.185 kb on - strand, within entF at 616.203 kb on + strand, within entF at 616.211 kb on + strand, within entF at 616.529 kb on + strand, within entF at 616.529 kb on + strand, within entF at 616.645 kb on + strand, within entF at 616.779 kb on - strand, within entF at 616.779 kb on - strand, within entF at 616.793 kb on - strand, within entF at 616.814 kb on + strand, within entF at 616.868 kb on + strand, within entF at 616.988 kb on - strand at 616.993 kb on - strand at 617.023 kb on - strand at 617.024 kb on + strand at 617.024 kb on + strand at 617.038 kb on - strand at 617.048 kb on + strand at 617.066 kb on - strand at 617.120 kb on + strand at 617.121 kb on + strand at 617.122 kb on + strand at 617.133 kb on + strand at 617.135 kb on - strand at 617.136 kb on - strand at 617.136 kb on - strand at 617.138 kb on + strand at 617.146 kb on - strand at 617.155 kb on + strand at 617.160 kb on - strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.162 kb on + strand at 617.166 kb on + strand at 617.168 kb on + strand at 617.168 kb on - strand at 617.170 kb on + strand at 617.175 kb on + strand at 617.175 kb on + strand at 617.181 kb on + strand at 617.181 kb on + strand at 617.182 kb on + strand at 617.183 kb on - strand at 617.195 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.206 kb on + strand at 617.235 kb on + strand at 617.247 kb on + strand at 617.272 kb on + strand at 617.587 kb on - strand at 617.616 kb on + strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.701 kb on - strand, within fepE at 617.755 kb on - strand, within fepE at 617.849 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.947 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 618.013 kb on + strand, within fepE at 618.013 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.029 kb on - strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.053 kb on - strand, within fepE at 618.071 kb on + strand, within fepE at 618.082 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.114 kb on + strand, within fepE at 618.120 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.190 kb on + strand, within fepE at 618.250 kb on - strand, within fepE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 612,424 + fes b0585 0.34 -2.9 612,424 + fes b0585 0.34 -3.4 612,629 - fes b0585 0.53 -3.1 612,629 - fes b0585 0.53 -3.5 612,676 - fes b0585 0.57 -3.9 612,835 + fes b0585 0.71 -2.4 612,840 + fes b0585 0.71 -3.0 612,843 - fes b0585 0.72 -3.1 612,843 - fes b0585 0.72 -1.8 612,868 - fes b0585 0.74 -2.6 612,869 - fes b0585 0.74 -2.8 612,869 - fes b0585 0.74 -2.3 612,871 + fes b0585 0.74 -3.1 612,883 + fes b0585 0.75 -2.9 612,960 - fes b0585 0.82 -2.1 613,018 + fes b0585 0.87 -4.7 613,018 + fes b0585 0.87 -1.8 613,181 - +0.2 613,181 - +0.3 613,203 + ybdZ b4511 0.17 +0.3 613,270 + ybdZ b4511 0.48 -0.4 613,340 + ybdZ b4511 0.80 -0.1 613,396 - -0.5 613,455 + -0.9 613,455 + -0.7 613,523 + +0.1 613,523 + +0.1 613,549 - -0.5 613,549 - -1.0 613,589 + +0.0 613,643 + -0.2 613,663 + -0.5 613,663 + -1.0 613,663 + -0.1 613,671 - -0.6 613,671 - -0.0 613,870 - entF b0586 0.13 -0.8 613,923 + entF b0586 0.14 -0.2 613,923 + entF b0586 0.14 -0.5 613,927 - entF b0586 0.14 -0.7 613,974 + entF b0586 0.15 -1.4 614,028 - entF b0586 0.17 -0.7 614,050 - entF b0586 0.17 -0.4 614,050 - entF b0586 0.17 -0.4 614,181 - entF b0586 0.21 -1.6 614,181 - entF b0586 0.21 -0.5 614,216 + entF b0586 0.22 +0.1 614,216 + entF b0586 0.22 +0.5 614,421 + entF b0586 0.27 -1.0 614,517 - entF b0586 0.29 -0.7 614,517 - entF b0586 0.29 -0.5 614,517 - entF b0586 0.29 -2.0 614,631 + entF b0586 0.32 -0.1 614,665 - entF b0586 0.33 +0.2 614,665 - entF b0586 0.33 -0.7 614,711 + entF b0586 0.34 +0.1 614,716 + entF b0586 0.34 -0.9 614,716 + entF b0586 0.34 -0.5 614,886 + entF b0586 0.39 -0.4 614,910 + entF b0586 0.39 -0.5 614,969 + entF b0586 0.41 -0.9 614,977 - entF b0586 0.41 -0.1 614,993 - entF b0586 0.42 -0.2 615,069 - entF b0586 0.44 -0.0 615,121 + entF b0586 0.45 -0.3 615,126 + entF b0586 0.45 -0.2 615,369 - entF b0586 0.51 -0.8 615,593 - entF b0586 0.57 -0.7 615,600 + entF b0586 0.57 -0.2 615,600 + entF b0586 0.57 -0.7 615,688 - entF b0586 0.59 -0.4 615,726 + entF b0586 0.60 +0.0 615,879 - entF b0586 0.64 -0.4 615,881 - entF b0586 0.64 -1.0 616,125 + entF b0586 0.71 -0.9 616,185 - entF b0586 0.72 +0.2 616,185 - entF b0586 0.72 -1.3 616,203 + entF b0586 0.73 +0.0 616,211 + entF b0586 0.73 -0.1 616,529 + entF b0586 0.81 -1.4 616,529 + entF b0586 0.81 -0.5 616,645 + entF b0586 0.84 +0.0 616,779 - entF b0586 0.88 +0.1 616,779 - entF b0586 0.88 -0.5 616,793 - entF b0586 0.88 -0.4 616,814 + entF b0586 0.88 -1.0 616,868 + entF b0586 0.90 -0.2 616,988 - -1.3 616,993 - -0.2 617,023 - -0.4 617,024 + -1.2 617,024 + -1.8 617,038 - -0.3 617,048 + -0.4 617,066 - -0.2 617,120 + -0.5 617,121 + -0.6 617,122 + +0.8 617,133 + -0.4 617,135 - +0.1 617,136 - -0.3 617,136 - -2.0 617,138 + -0.3 617,146 - -1.3 617,155 + -0.9 617,160 - +0.9 617,162 + -0.6 617,162 + -1.5 617,162 + -0.2 617,166 + +0.2 617,168 + -0.1 617,168 - +0.2 617,170 + -0.2 617,175 + +0.2 617,175 + -0.8 617,181 + -0.3 617,181 + -1.7 617,182 + -0.6 617,183 - +0.5 617,195 + -0.3 617,206 + -0.0 617,206 + +0.9 617,206 + -0.2 617,206 + -1.2 617,206 + -0.0 617,206 + -0.5 617,235 + -0.0 617,247 + -0.3 617,272 + +0.4 617,587 - -0.6 617,616 + fepE b0587 0.12 +2.5 617,644 - fepE b0587 0.15 +0.4 617,644 - fepE b0587 0.15 +0.1 617,644 - fepE b0587 0.15 +0.5 617,701 - fepE b0587 0.20 -0.3 617,755 - fepE b0587 0.25 -1.1 617,849 + fepE b0587 0.33 +0.1 617,893 + fepE b0587 0.37 -0.6 617,893 + fepE b0587 0.37 +1.8 617,947 - fepE b0587 0.41 +2.5 617,985 - fepE b0587 0.45 +1.5 617,985 - fepE b0587 0.45 +0.9 617,986 - fepE b0587 0.45 +0.4 618,013 + fepE b0587 0.47 -0.2 618,013 + fepE b0587 0.47 +0.6 618,018 + fepE b0587 0.48 +1.5 618,018 + fepE b0587 0.48 +1.9 618,029 - fepE b0587 0.49 +0.5 618,035 + fepE b0587 0.49 +0.2 618,035 + fepE b0587 0.49 -0.3 618,035 + fepE b0587 0.49 -0.1 618,045 + fepE b0587 0.50 +1.8 618,045 + fepE b0587 0.50 +0.7 618,053 - fepE b0587 0.51 +2.5 618,071 + fepE b0587 0.52 -0.8 618,082 + fepE b0587 0.53 -1.4 618,104 + fepE b0587 0.55 -0.7 618,104 + fepE b0587 0.55 +1.5 618,114 + fepE b0587 0.56 +2.1 618,120 - fepE b0587 0.57 +1.5 618,151 - fepE b0587 0.59 +1.2 618,151 - fepE b0587 0.59 +0.9 618,190 + fepE b0587 0.63 -0.4 618,250 - fepE b0587 0.68 +0.8
Or see this region's nucleotide sequence