Strain Fitness in Escherichia coli BW25113 around b0143

Experiment: Ying_Nucleobase14 rep C; time point 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_Nucleobase14 rep C; time point 2
156,734	+	yadN	b0141	0.74	+0.9
156,735	+	yadN	b0141	0.75	+1.1
156,735	+	yadN	b0141	0.75	+0.6
156,744	+	yadN	b0141	0.76	+1.3
156,755	+	yadN	b0141	0.78	-0.2
156,757	+	yadN	b0141	0.78	+0.3
156,759	+	yadN	b0141	0.79	-0.3
156,786	+	yadN	b0141	0.83	+2.7
156,789	+	yadN	b0141	0.84	-1.4
156,793	+	yadN	b0141	0.84	+0.2
156,804	+	yadN	b0141	0.86	-1.1
156,823	+	yadN	b0141	0.90	-0.8
156,823	+	yadN	b0141	0.90	+1.4
156,825	+				-1.0
156,827	+				+0.1
156,827	+				+0.4
156,834	+				+0.7
156,849	+				+0.2
156,849	+				-0.5
156,849	+				-0.8
156,850	+				-0.0
156,854	+				-0.5
156,854	+				-0.9
157,218	-				+1.7
157,279	-				+0.1
157,291	-				+1.0
157,301	-	folK	b0142	0.10	-1.9
157,745	+				-1.4
157,870	-	pcnB	b0143	0.10	-0.4
157,906	-	pcnB	b0143	0.13	+0.0
157,906	-	pcnB	b0143	0.13	-0.1
157,923	-	pcnB	b0143	0.14	-0.7
158,143	+	pcnB	b0143	0.30	-1.5
158,156	+	pcnB	b0143	0.31	-1.7
158,227	-	pcnB	b0143	0.36	-0.7
158,249	-	pcnB	b0143	0.37	-0.7
158,249	-	pcnB	b0143	0.37	-1.1
158,312	-	pcnB	b0143	0.42	-1.4
158,342	-	pcnB	b0143	0.44	-1.3
158,378	-	pcnB	b0143	0.46	-3.1
158,400	+	pcnB	b0143	0.48	-2.4
158,408	-	pcnB	b0143	0.49	-0.8
158,408	-	pcnB	b0143	0.49	-1.0
158,426	-	pcnB	b0143	0.50	-0.1
158,442	-	pcnB	b0143	0.51	-2.9
158,442	-	pcnB	b0143	0.51	-0.5
158,447	-	pcnB	b0143	0.51	-2.6
158,516	+	pcnB	b0143	0.56	-1.3
158,522	-	pcnB	b0143	0.57	-3.4
158,759	-	pcnB	b0143	0.74	-0.9
158,759	-	pcnB	b0143	0.74	-3.0
158,759	-	pcnB	b0143	0.74	-2.0
158,864	-	pcnB	b0143	0.81	-2.1
159,038	-				-1.9
159,093	-				+0.1
159,093	-				-1.1
159,133	+				-0.7
159,347	-	yadB	b0144	0.17	+0.3
159,347	-	yadB	b0144	0.17	-1.2
159,352	-	yadB	b0144	0.18	+0.7
159,352	-	yadB	b0144	0.18	+0.4
159,581	-	yadB	b0144	0.43	+0.4
159,655	+	yadB	b0144	0.51	-0.4
159,699	+	yadB	b0144	0.55	+0.5
159,722	-	yadB	b0144	0.58	+0.6
159,722	-	yadB	b0144	0.58	+0.0
159,743	-	yadB	b0144	0.60	-0.6
159,754	-	yadB	b0144	0.61	+0.3
159,754	-	yadB	b0144	0.61	+0.0
159,770	+	yadB	b0144	0.63	-0.5
159,770	+	yadB	b0144	0.63	+0.2
159,803	+	yadB	b0144	0.67	+0.0
159,808	+	yadB	b0144	0.67	+0.3
159,905	-	yadB	b0144	0.78	+0.3
159,947	+	yadB	b0144	0.82	-0.1
160,046	+				-0.4
160,068	+				-0.1
160,068	-				-1.2
160,068	-				+0.4
160,070	-				-0.3
160,078	+				-0.3
160,078	+				-0.4