Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ampD and ampE overlap by 4 nucleotides ampE and aroP are separated by 42 nucleotides aroP and pdhR are separated by 540 nucleotides
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
b0113: pdhR - transcriptional regulator of pyruvate dehydrogenase complex (NCBI), at 122,092 to 122,856
pdhR
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE at 119.753 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.756 kb on + strand, within ampE at 119.772 kb on - strand, within ampE at 119.786 kb on + strand, within ampE at 119.786 kb on + strand, within ampE at 119.799 kb on - strand, within ampE at 119.799 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.915 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.983 kb on + strand, within ampE at 119.985 kb on - strand, within ampE at 120.006 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.037 kb on - strand, within ampE at 120.138 kb on - strand at 120.139 kb on + strand at 120.153 kb on - strand at 120.155 kb on + strand at 120.237 kb on - strand at 120.251 kb on - strand at 120.254 kb on + strand at 120.266 kb on - strand at 120.266 kb on - strand at 120.340 kb on - strand, within aroP at 120.362 kb on - strand, within aroP at 120.377 kb on + strand, within aroP at 120.389 kb on - strand, within aroP at 120.433 kb on + strand, within aroP at 120.461 kb on + strand, within aroP at 120.484 kb on + strand, within aroP at 120.502 kb on - strand, within aroP at 120.502 kb on - strand, within aroP at 120.514 kb on - strand, within aroP at 120.514 kb on - strand, within aroP at 120.518 kb on + strand, within aroP at 120.560 kb on - strand, within aroP at 120.571 kb on - strand, within aroP at 120.611 kb on - strand, within aroP at 120.611 kb on - strand, within aroP at 120.647 kb on - strand, within aroP at 120.647 kb on - strand, within aroP at 120.675 kb on + strand, within aroP at 120.683 kb on - strand, within aroP at 120.706 kb on + strand, within aroP at 120.712 kb on + strand, within aroP at 120.712 kb on + strand, within aroP at 120.752 kb on + strand, within aroP at 120.753 kb on - strand, within aroP at 120.753 kb on - strand, within aroP at 120.847 kb on + strand, within aroP at 120.854 kb on - strand, within aroP at 120.905 kb on - strand, within aroP at 120.905 kb on - strand, within aroP at 120.956 kb on - strand, within aroP at 120.956 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.032 kb on - strand, within aroP at 121.054 kb on + strand, within aroP at 121.087 kb on + strand, within aroP at 121.095 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.128 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.146 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.266 kb on - strand, within aroP at 121.335 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.374 kb on - strand, within aroP at 121.431 kb on + strand at 121.431 kb on + strand at 121.458 kb on - strand at 121.469 kb on - strand at 121.514 kb on + strand at 121.531 kb on - strand at 121.716 kb on - strand at 121.716 kb on - strand at 121.723 kb on - strand at 121.723 kb on - strand at 121.738 kb on - strand at 122.355 kb on + strand, within pdhR at 122.358 kb on + strand, within pdhR at 122.369 kb on + strand, within pdhR at 122.416 kb on + strand, within pdhR at 122.442 kb on + strand, within pdhR at 122.471 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.489 kb on + strand, within pdhR at 122.500 kb on + strand, within pdhR at 122.537 kb on + strand, within pdhR
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 119,191 + ampD b0110 0.83 +0.1 119,191 + ampD b0110 0.83 -0.4 119,274 + +0.0 119,339 + -0.5 119,339 + +0.1 119,499 - ampE b0111 0.25 -4.5 119,499 - ampE b0111 0.25 -0.2 119,522 + ampE b0111 0.28 -3.0 119,528 - ampE b0111 0.29 -0.5 119,555 - ampE b0111 0.32 +0.3 119,555 - ampE b0111 0.32 -0.0 119,558 - ampE b0111 0.32 +0.6 119,567 + ampE b0111 0.33 -0.7 119,567 + ampE b0111 0.33 +0.5 119,753 - ampE b0111 0.55 -0.3 119,755 - ampE b0111 0.55 +0.5 119,755 - ampE b0111 0.55 -0.1 119,756 + ampE b0111 0.56 +0.4 119,772 - ampE b0111 0.57 -0.1 119,786 + ampE b0111 0.59 -0.1 119,786 + ampE b0111 0.59 -0.2 119,799 - ampE b0111 0.61 +0.3 119,799 - ampE b0111 0.61 -0.4 119,836 - ampE b0111 0.65 +0.3 119,836 - ampE b0111 0.65 -0.3 119,915 + ampE b0111 0.74 +0.3 119,961 + ampE b0111 0.80 -0.1 119,961 + ampE b0111 0.80 -0.7 119,983 + ampE b0111 0.82 +0.2 119,985 - ampE b0111 0.82 -0.6 120,006 - ampE b0111 0.85 +0.6 120,013 - ampE b0111 0.86 -0.2 120,013 - ampE b0111 0.86 -1.9 120,014 + ampE b0111 0.86 -0.9 120,014 + ampE b0111 0.86 -0.4 120,014 + ampE b0111 0.86 -0.1 120,014 + ampE b0111 0.86 +0.3 120,037 - ampE b0111 0.88 -0.0 120,138 - -0.2 120,139 + -0.4 120,153 - +0.0 120,155 + +0.0 120,237 - -0.3 120,251 - -0.0 120,254 + +0.2 120,266 - -0.3 120,266 - -1.2 120,340 - aroP b0112 0.12 -0.2 120,362 - aroP b0112 0.13 -0.5 120,377 + aroP b0112 0.14 -0.2 120,389 - aroP b0112 0.15 -0.3 120,433 + aroP b0112 0.19 +0.5 120,461 + aroP b0112 0.21 -0.1 120,484 + aroP b0112 0.22 -0.0 120,502 - aroP b0112 0.24 -0.8 120,502 - aroP b0112 0.24 -0.6 120,514 - aroP b0112 0.24 -0.2 120,514 - aroP b0112 0.24 -0.4 120,518 + aroP b0112 0.25 +0.1 120,560 - aroP b0112 0.28 -0.1 120,571 - aroP b0112 0.29 -4.8 120,611 - aroP b0112 0.32 -1.0 120,611 - aroP b0112 0.32 -0.9 120,647 - aroP b0112 0.34 +0.2 120,647 - aroP b0112 0.34 -0.6 120,675 + aroP b0112 0.36 -0.2 120,683 - aroP b0112 0.37 -0.4 120,706 + aroP b0112 0.38 -0.2 120,712 + aroP b0112 0.39 +0.1 120,712 + aroP b0112 0.39 -0.4 120,752 + aroP b0112 0.42 -0.6 120,753 - aroP b0112 0.42 -0.3 120,753 - aroP b0112 0.42 -0.3 120,847 + aroP b0112 0.49 -0.5 120,854 - aroP b0112 0.49 -0.7 120,905 - aroP b0112 0.53 -0.5 120,905 - aroP b0112 0.53 -0.6 120,956 - aroP b0112 0.57 -0.5 120,956 - aroP b0112 0.57 -0.9 121,032 - aroP b0112 0.62 -0.3 121,032 - aroP b0112 0.62 -0.2 121,032 - aroP b0112 0.62 +0.1 121,054 + aroP b0112 0.64 -0.0 121,087 + aroP b0112 0.66 +0.2 121,095 - aroP b0112 0.67 -0.6 121,128 - aroP b0112 0.69 -0.0 121,128 - aroP b0112 0.69 -0.0 121,146 - aroP b0112 0.70 -0.5 121,146 - aroP b0112 0.70 -0.8 121,266 - aroP b0112 0.79 -0.7 121,266 - aroP b0112 0.79 +0.3 121,335 - aroP b0112 0.84 -0.1 121,374 - aroP b0112 0.87 -0.5 121,374 - aroP b0112 0.87 -0.3 121,431 + +1.3 121,431 + -0.3 121,458 - -0.4 121,469 - -0.1 121,514 + -0.4 121,531 - -0.2 121,716 - +0.1 121,716 - +0.3 121,723 - +0.2 121,723 - +0.1 121,738 - +0.2 122,355 + pdhR b0113 0.34 -4.1 122,358 + pdhR b0113 0.35 -3.3 122,369 + pdhR b0113 0.36 -2.5 122,416 + pdhR b0113 0.42 -3.0 122,442 + pdhR b0113 0.46 -4.7 122,471 + pdhR b0113 0.50 -5.1 122,489 + pdhR b0113 0.52 -3.6 122,489 + pdhR b0113 0.52 -3.2 122,489 + pdhR b0113 0.52 -1.6 122,500 + pdhR b0113 0.53 -1.9 122,537 + pdhR b0113 0.58 -4.3
Or see this region's nucleotide sequence