Strain Fitness in Escherichia coli BW25113 around b2423

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysA and cysW overlap by 11 nucleotidescysW and cysU overlap by 1 nucleotidescysU and cysP overlap by 1 nucleotides b2422: cysA - sulfate/thiosulfate transporter subunit (NCBI), at 2,537,739 to 2,538,836 cysA b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701 cysW b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534 cysU b2425: cysP - thiosulfate transporter subunit (NCBI), at 2,540,534 to 2,541,550 cysP Position (kb) 2538 2539 2540Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2537.849 kb on + strand, within cysAat 2537.862 kb on - strand, within cysAat 2537.903 kb on - strand, within cysAat 2537.963 kb on - strand, within cysAat 2538.033 kb on + strand, within cysAat 2538.037 kb on - strand, within cysAat 2538.054 kb on - strand, within cysAat 2538.054 kb on - strand, within cysAat 2538.084 kb on - strand, within cysAat 2538.108 kb on - strand, within cysAat 2538.114 kb on + strand, within cysAat 2538.193 kb on + strand, within cysAat 2538.193 kb on + strand, within cysAat 2538.224 kb on + strand, within cysAat 2538.224 kb on + strand, within cysAat 2538.334 kb on - strand, within cysAat 2538.334 kb on - strand, within cysAat 2538.336 kb on - strand, within cysAat 2538.699 kb on + strand, within cysAat 2538.824 kb on + strandat 2538.824 kb on + strandat 2538.846 kb on - strandat 2538.846 kb on - strandat 2538.952 kb on + strand, within cysWat 2538.952 kb on + strand, within cysWat 2538.959 kb on + strand, within cysWat 2538.961 kb on + strand, within cysWat 2538.986 kb on + strand, within cysWat 2539.072 kb on + strand, within cysWat 2539.173 kb on + strand, within cysWat 2539.173 kb on + strand, within cysWat 2539.240 kb on + strand, within cysWat 2539.249 kb on - strand, within cysWat 2539.249 kb on - strand, within cysWat 2539.456 kb on + strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.694 kb on - strandat 2540.062 kb on + strand, within cysUat 2540.110 kb on - strand, within cysUat 2540.110 kb on - strand, within cysUat 2540.129 kb on + strand, within cysUat 2540.492 kb on + strandat 2540.535 kb on + strandat 2540.563 kb on - strandat 2540.677 kb on - strand, within cysPat 2540.677 kb on - strand, within cysPat 2540.695 kb on - strand, within cysP

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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2,537,849 + cysA b2422 0.10 -1.7
2,537,862 - cysA b2422 0.11 -1.2
2,537,903 - cysA b2422 0.15 -0.7
2,537,963 - cysA b2422 0.20 -1.9
2,538,033 + cysA b2422 0.27 -2.1
2,538,037 - cysA b2422 0.27 -1.7
2,538,054 - cysA b2422 0.29 -3.5
2,538,054 - cysA b2422 0.29 -1.9
2,538,084 - cysA b2422 0.31 -1.7
2,538,108 - cysA b2422 0.34 -2.1
2,538,114 + cysA b2422 0.34 -2.4
2,538,193 + cysA b2422 0.41 -2.8
2,538,193 + cysA b2422 0.41 -0.3
2,538,224 + cysA b2422 0.44 -2.7
2,538,224 + cysA b2422 0.44 -1.0
2,538,334 - cysA b2422 0.54 -4.4
2,538,334 - cysA b2422 0.54 -1.3
2,538,336 - cysA b2422 0.54 -0.8
2,538,699 + cysA b2422 0.87 -3.1
2,538,824 + -0.8
2,538,824 + -2.1
2,538,846 - -1.3
2,538,846 - -2.2
2,538,952 + cysW b2423 0.14 -0.5
2,538,952 + cysW b2423 0.14 -1.9
2,538,959 + cysW b2423 0.15 -3.3
2,538,961 + cysW b2423 0.15 -3.4
2,538,986 + cysW b2423 0.18 -3.9
2,539,072 + cysW b2423 0.28 -1.8
2,539,173 + cysW b2423 0.40 -3.0
2,539,173 + cysW b2423 0.40 -1.3
2,539,240 + cysW b2423 0.47 -1.5
2,539,249 - cysW b2423 0.48 -1.4
2,539,249 - cysW b2423 0.48 -1.2
2,539,456 + cysW b2423 0.72 -0.7
2,539,468 - cysW b2423 0.73 -0.4
2,539,468 - cysW b2423 0.73 -2.0
2,539,468 - cysW b2423 0.73 -2.4
2,539,694 - -0.5
2,540,062 + cysU b2424 0.43 -2.2
2,540,110 - cysU b2424 0.49 -1.7
2,540,110 - cysU b2424 0.49 -1.5
2,540,129 + cysU b2424 0.51 -2.0
2,540,492 + -2.3
2,540,535 + -1.6
2,540,563 - -1.1
2,540,677 - cysP b2425 0.14 +0.2
2,540,677 - cysP b2425 0.14 -0.3
2,540,695 - cysP b2425 0.16 +0.4

Or see this region's nucleotide sequence