Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysA and cysW overlap by 11 nucleotides cysW and cysU overlap by 1 nucleotides cysU and cysP overlap by 1 nucleotides
b2422: cysA - sulfate/thiosulfate transporter subunit (NCBI), at 2,537,739 to 2,538,836
cysA
b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701
cysW
b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534
cysU
b2425: cysP - thiosulfate transporter subunit (NCBI), at 2,540,534 to 2,541,550
cysP
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2537.849 kb on + strand, within cysA at 2537.862 kb on - strand, within cysA at 2537.903 kb on - strand, within cysA at 2537.963 kb on - strand, within cysA at 2538.033 kb on + strand, within cysA at 2538.037 kb on - strand, within cysA at 2538.054 kb on - strand, within cysA at 2538.054 kb on - strand, within cysA at 2538.084 kb on - strand, within cysA at 2538.108 kb on - strand, within cysA at 2538.114 kb on + strand, within cysA at 2538.193 kb on + strand, within cysA at 2538.193 kb on + strand, within cysA at 2538.224 kb on + strand, within cysA at 2538.224 kb on + strand, within cysA at 2538.334 kb on - strand, within cysA at 2538.334 kb on - strand, within cysA at 2538.336 kb on - strand, within cysA at 2538.699 kb on + strand, within cysA at 2538.824 kb on + strand at 2538.824 kb on + strand at 2538.846 kb on - strand at 2538.846 kb on - strand at 2538.952 kb on + strand, within cysW at 2538.952 kb on + strand, within cysW at 2538.959 kb on + strand, within cysW at 2538.961 kb on + strand, within cysW at 2538.986 kb on + strand, within cysW at 2539.072 kb on + strand, within cysW at 2539.173 kb on + strand, within cysW at 2539.173 kb on + strand, within cysW at 2539.240 kb on + strand, within cysW at 2539.249 kb on - strand, within cysW at 2539.249 kb on - strand, within cysW at 2539.456 kb on + strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.694 kb on - strand at 2540.062 kb on + strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.129 kb on + strand, within cysU at 2540.492 kb on + strand at 2540.535 kb on + strand at 2540.563 kb on - strand at 2540.677 kb on - strand, within cysP at 2540.677 kb on - strand, within cysP at 2540.695 kb on - strand, within cysP
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,537,849 + cysA b2422 0.10 -1.7 2,537,862 - cysA b2422 0.11 -1.2 2,537,903 - cysA b2422 0.15 -0.7 2,537,963 - cysA b2422 0.20 -1.9 2,538,033 + cysA b2422 0.27 -2.1 2,538,037 - cysA b2422 0.27 -1.7 2,538,054 - cysA b2422 0.29 -3.5 2,538,054 - cysA b2422 0.29 -1.9 2,538,084 - cysA b2422 0.31 -1.7 2,538,108 - cysA b2422 0.34 -2.1 2,538,114 + cysA b2422 0.34 -2.4 2,538,193 + cysA b2422 0.41 -2.8 2,538,193 + cysA b2422 0.41 -0.3 2,538,224 + cysA b2422 0.44 -2.7 2,538,224 + cysA b2422 0.44 -1.0 2,538,334 - cysA b2422 0.54 -4.4 2,538,334 - cysA b2422 0.54 -1.3 2,538,336 - cysA b2422 0.54 -0.8 2,538,699 + cysA b2422 0.87 -3.1 2,538,824 + -0.8 2,538,824 + -2.1 2,538,846 - -1.3 2,538,846 - -2.2 2,538,952 + cysW b2423 0.14 -0.5 2,538,952 + cysW b2423 0.14 -1.9 2,538,959 + cysW b2423 0.15 -3.3 2,538,961 + cysW b2423 0.15 -3.4 2,538,986 + cysW b2423 0.18 -3.9 2,539,072 + cysW b2423 0.28 -1.8 2,539,173 + cysW b2423 0.40 -3.0 2,539,173 + cysW b2423 0.40 -1.3 2,539,240 + cysW b2423 0.47 -1.5 2,539,249 - cysW b2423 0.48 -1.4 2,539,249 - cysW b2423 0.48 -1.2 2,539,456 + cysW b2423 0.72 -0.7 2,539,468 - cysW b2423 0.73 -0.4 2,539,468 - cysW b2423 0.73 -2.0 2,539,468 - cysW b2423 0.73 -2.4 2,539,694 - -0.5 2,540,062 + cysU b2424 0.43 -2.2 2,540,110 - cysU b2424 0.49 -1.7 2,540,110 - cysU b2424 0.49 -1.5 2,540,129 + cysU b2424 0.51 -2.0 2,540,492 + -2.3 2,540,535 + -1.6 2,540,563 - -1.1 2,540,677 - cysP b2425 0.14 +0.2 2,540,677 - cysP b2425 0.14 -0.3 2,540,695 - cysP b2425 0.16 +0.4
Or see this region's nucleotide sequence