Strain Fitness in Escherichia coli BW25113 around b1663

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcfa and ribC are separated by 39 nucleotidesribC and mdtK are separated by 214 nucleotidesmdtK and ydhQ are separated by 40 nucleotides b1661: cfa - cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI), at 1,739,437 to 1,740,585 cfa b1662: ribC - riboflavin synthase subunit alpha (NCBI), at 1,740,625 to 1,741,266 ribC b1663: mdtK - multidrug efflux protein NorM (NCBI), at 1,741,481 to 1,742,854 mdtK b1664: ydhQ - hypothetical protein (NCBI), at 1,742,895 to 1,744,151 ydhQ Position (kb) 1741 1742 1743Strain fitness (log2 ratio) -1 0 1 2 3 4at 1740.505 kb on - strandat 1740.538 kb on + strandat 1741.305 kb on + strandat 1741.305 kb on + strandat 1741.328 kb on - strandat 1741.333 kb on - strandat 1741.342 kb on + strandat 1741.736 kb on - strand, within mdtKat 1741.736 kb on - strand, within mdtKat 1741.798 kb on + strand, within mdtKat 1741.798 kb on + strand, within mdtKat 1741.928 kb on - strand, within mdtKat 1742.088 kb on - strand, within mdtKat 1742.235 kb on + strand, within mdtKat 1742.237 kb on + strand, within mdtKat 1742.253 kb on - strand, within mdtKat 1742.299 kb on + strand, within mdtKat 1742.419 kb on - strand, within mdtKat 1742.436 kb on - strand, within mdtKat 1742.514 kb on - strand, within mdtKat 1742.528 kb on + strand, within mdtKat 1742.696 kb on - strand, within mdtKat 1742.696 kb on - strand, within mdtKat 1742.902 kb on + strandat 1742.950 kb on + strandat 1743.088 kb on + strand, within ydhQat 1743.088 kb on + strand, within ydhQat 1743.220 kb on + strand, within ydhQat 1743.220 kb on + strand, within ydhQat 1743.225 kb on + strand, within ydhQat 1743.230 kb on + strand, within ydhQat 1743.233 kb on - strand, within ydhQat 1743.380 kb on + strand, within ydhQat 1743.398 kb on - strand, within ydhQat 1743.446 kb on + strand, within ydhQat 1743.446 kb on + strand, within ydhQat 1743.596 kb on - strand, within ydhQat 1743.597 kb on - strand, within ydhQat 1743.597 kb on - strand, within ydhQat 1743.666 kb on - strand, within ydhQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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1,740,505 - -0.1
1,740,538 + +0.2
1,741,305 + -0.5
1,741,305 + -0.2
1,741,328 - -0.1
1,741,333 - +0.1
1,741,342 + -1.5
1,741,736 - mdtK b1663 0.19 +1.3
1,741,736 - mdtK b1663 0.19 +2.3
1,741,798 + mdtK b1663 0.23 +1.5
1,741,798 + mdtK b1663 0.23 +1.1
1,741,928 - mdtK b1663 0.33 +1.3
1,742,088 - mdtK b1663 0.44 +1.4
1,742,235 + mdtK b1663 0.55 +1.4
1,742,237 + mdtK b1663 0.55 +0.8
1,742,253 - mdtK b1663 0.56 +3.8
1,742,299 + mdtK b1663 0.60 +2.3
1,742,419 - mdtK b1663 0.68 +0.8
1,742,436 - mdtK b1663 0.70 +1.4
1,742,514 - mdtK b1663 0.75 +1.2
1,742,528 + mdtK b1663 0.76 +1.4
1,742,696 - mdtK b1663 0.88 +1.5
1,742,696 - mdtK b1663 0.88 +2.1
1,742,902 + -0.3
1,742,950 + -0.9
1,743,088 + ydhQ b1664 0.15 +0.2
1,743,088 + ydhQ b1664 0.15 +1.1
1,743,220 + ydhQ b1664 0.26 -0.5
1,743,220 + ydhQ b1664 0.26 +0.8
1,743,225 + ydhQ b1664 0.26 +0.8
1,743,230 + ydhQ b1664 0.27 +0.0
1,743,233 - ydhQ b1664 0.27 -1.0
1,743,380 + ydhQ b1664 0.39 +0.4
1,743,398 - ydhQ b1664 0.40 +0.4
1,743,446 + ydhQ b1664 0.44 -1.1
1,743,446 + ydhQ b1664 0.44 -0.8
1,743,596 - ydhQ b1664 0.56 +0.6
1,743,597 - ydhQ b1664 0.56 +0.8
1,743,597 - ydhQ b1664 0.56 +0.4
1,743,666 - ydhQ b1664 0.61 -0.5

Or see this region's nucleotide sequence