Strain Fitness in Escherichia coli BW25113 around b4246

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrI and pyrB are separated by 12 nucleotidespyrB and pyrL are separated by 3 nucleotidespyrL and yjgH are separated by 280 nucleotidesyjgH and yjgI are separated by 130 nucleotides b4244: pyrI - aspartate carbamoyltransferase regulatory subunit (NCBI), at 4,469,009 to 4,469,470 pyrI b4245: pyrB - aspartate carbamoyltransferase catalytic subunit (NCBI), at 4,469,483 to 4,470,418 pyrB b4246: pyrL - pyrBI operon leader peptide (NCBI), at 4,470,422 to 4,470,556 pyrL b4248: yjgH - predicted mRNA endoribonuclease (NCBI), at 4,470,837 to 4,471,232 yjgH b4249: yjgI - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 4,471,363 to 4,472,076 yjgI Position (kb) 4470 4471Strain fitness (log2 ratio) -1 0 1 2at 4469.422 kb on - strand, within pyrIat 4469.442 kb on - strandat 4469.501 kb on + strandat 4469.560 kb on - strandat 4469.562 kb on + strandat 4469.579 kb on + strand, within pyrBat 4469.579 kb on + strand, within pyrBat 4469.648 kb on + strand, within pyrBat 4469.715 kb on - strand, within pyrBat 4469.744 kb on - strand, within pyrBat 4469.746 kb on - strand, within pyrBat 4469.751 kb on + strand, within pyrBat 4469.777 kb on + strand, within pyrBat 4469.780 kb on + strand, within pyrBat 4469.780 kb on + strand, within pyrBat 4469.787 kb on + strand, within pyrBat 4469.793 kb on - strand, within pyrBat 4469.793 kb on - strand, within pyrBat 4469.817 kb on + strand, within pyrBat 4469.937 kb on - strand, within pyrBat 4469.945 kb on + strand, within pyrBat 4469.952 kb on + strand, within pyrBat 4469.966 kb on + strand, within pyrBat 4470.043 kb on + strand, within pyrBat 4470.104 kb on + strand, within pyrBat 4470.104 kb on + strand, within pyrBat 4470.106 kb on + strand, within pyrBat 4470.200 kb on + strand, within pyrBat 4470.224 kb on + strand, within pyrBat 4470.257 kb on + strand, within pyrBat 4470.359 kb on - strandat 4470.427 kb on - strandat 4470.427 kb on - strandat 4470.466 kb on + strand, within pyrLat 4470.466 kb on + strand, within pyrLat 4470.482 kb on - strand, within pyrLat 4470.718 kb on + strandat 4470.764 kb on + strandat 4470.783 kb on - strandat 4470.783 kb on - strandat 4470.796 kb on + strandat 4470.796 kb on + strandat 4470.808 kb on + strandat 4470.830 kb on + strandat 4470.830 kb on - strandat 4470.830 kb on - strandat 4470.840 kb on + strandat 4470.842 kb on + strandat 4470.842 kb on + strandat 4470.842 kb on - strandat 4470.898 kb on - strand, within yjgHat 4470.899 kb on - strand, within yjgHat 4470.988 kb on + strand, within yjgHat 4470.996 kb on - strand, within yjgHat 4470.996 kb on - strand, within yjgHat 4470.996 kb on - strand, within yjgHat 4471.004 kb on - strand, within yjgHat 4471.009 kb on - strand, within yjgHat 4471.012 kb on - strand, within yjgHat 4471.097 kb on + strand, within yjgHat 4471.176 kb on - strand, within yjgHat 4471.354 kb on - strandat 4471.362 kb on + strandat 4471.367 kb on + strandat 4471.378 kb on - strandat 4471.388 kb on - strandat 4471.388 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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4,469,422 - pyrI b4244 0.89 +0.6
4,469,442 - +0.5
4,469,501 + -0.0
4,469,560 - -0.2
4,469,562 + +1.6
4,469,579 + pyrB b4245 0.10 +0.2
4,469,579 + pyrB b4245 0.10 +0.2
4,469,648 + pyrB b4245 0.18 +0.5
4,469,715 - pyrB b4245 0.25 -0.1
4,469,744 - pyrB b4245 0.28 -0.5
4,469,746 - pyrB b4245 0.28 -0.1
4,469,751 + pyrB b4245 0.29 +0.3
4,469,777 + pyrB b4245 0.31 +0.9
4,469,780 + pyrB b4245 0.32 +0.3
4,469,780 + pyrB b4245 0.32 +1.0
4,469,787 + pyrB b4245 0.32 +0.2
4,469,793 - pyrB b4245 0.33 -0.0
4,469,793 - pyrB b4245 0.33 -0.5
4,469,817 + pyrB b4245 0.36 +1.1
4,469,937 - pyrB b4245 0.49 +0.2
4,469,945 + pyrB b4245 0.49 -0.0
4,469,952 + pyrB b4245 0.50 +0.1
4,469,966 + pyrB b4245 0.52 -0.5
4,470,043 + pyrB b4245 0.60 +1.0
4,470,104 + pyrB b4245 0.66 -0.1
4,470,104 + pyrB b4245 0.66 +0.0
4,470,106 + pyrB b4245 0.67 +0.0
4,470,200 + pyrB b4245 0.77 +1.6
4,470,224 + pyrB b4245 0.79 +0.1
4,470,257 + pyrB b4245 0.83 -0.0
4,470,359 - +0.5
4,470,427 - -0.7
4,470,427 - -0.4
4,470,466 + pyrL b4246 0.33 +0.4
4,470,466 + pyrL b4246 0.33 +0.4
4,470,482 - pyrL b4246 0.44 +0.6
4,470,718 + +1.6
4,470,764 + -0.0
4,470,783 - -0.4
4,470,783 - +0.2
4,470,796 + -0.6
4,470,796 + +0.8
4,470,808 + -0.4
4,470,830 + +0.1
4,470,830 - +1.4
4,470,830 - -0.6
4,470,840 + -0.1
4,470,842 + -0.1
4,470,842 + +0.6
4,470,842 - +0.6
4,470,898 - yjgH b4248 0.15 +0.1
4,470,899 - yjgH b4248 0.16 -0.4
4,470,988 + yjgH b4248 0.38 +0.2
4,470,996 - yjgH b4248 0.40 -0.3
4,470,996 - yjgH b4248 0.40 -0.4
4,470,996 - yjgH b4248 0.40 -0.2
4,471,004 - yjgH b4248 0.42 -0.6
4,471,009 - yjgH b4248 0.43 -0.1
4,471,012 - yjgH b4248 0.44 -0.2
4,471,097 + yjgH b4248 0.66 -0.0
4,471,176 - yjgH b4248 0.86 -0.3
4,471,354 - +0.1
4,471,362 + +2.7
4,471,367 + -0.1
4,471,378 - -0.5
4,471,388 - -0.9
4,471,388 - +0.3

Or see this region's nucleotide sequence