Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjfO and yjfP are separated by 181 nucleotides yjfP and ulaR overlap by 4 nucleotides ulaR and yjfR are separated by 107 nucleotides
b4189: yjfO - orf, hypothetical protein (VIMSS), at 4,414,464 to 4,414,793
yjfO
b4190: yjfP - predicted hydrolase (NCBI), at 4,414,975 to 4,415,724
yjfP
b4191: ulaR - DNA-binding transcriptional dual regulator (NCBI), at 4,415,721 to 4,416,476
ulaR
b4192: yjfR - orf, hypothetical protein (VIMSS), at 4,416,584 to 4,417,648
yjfR
Position (kb)
4415
4416
4417 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4414.722 kb on - strand, within yjfO at 4414.746 kb on - strand, within yjfO at 4414.791 kb on - strand at 4414.791 kb on - strand at 4414.795 kb on + strand at 4414.800 kb on - strand at 4414.800 kb on - strand at 4414.825 kb on - strand at 4414.838 kb on - strand at 4414.838 kb on - strand at 4414.885 kb on + strand at 4414.899 kb on + strand at 4414.899 kb on + strand at 4415.030 kb on - strand at 4415.030 kb on - strand at 4415.050 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.058 kb on - strand, within yjfP at 4415.086 kb on - strand, within yjfP at 4415.103 kb on - strand, within yjfP at 4415.171 kb on + strand, within yjfP at 4415.171 kb on + strand, within yjfP at 4415.370 kb on + strand, within yjfP at 4415.370 kb on + strand, within yjfP at 4415.399 kb on + strand, within yjfP at 4415.399 kb on + strand, within yjfP at 4415.526 kb on - strand, within yjfP at 4415.562 kb on - strand, within yjfP at 4415.590 kb on - strand, within yjfP at 4415.620 kb on + strand, within yjfP at 4415.620 kb on + strand, within yjfP at 4415.632 kb on + strand, within yjfP at 4415.632 kb on + strand, within yjfP at 4415.657 kb on - strand at 4415.724 kb on - strand at 4415.724 kb on - strand at 4415.767 kb on - strand at 4415.767 kb on - strand at 4415.820 kb on + strand, within ulaR at 4415.879 kb on + strand, within ulaR at 4415.879 kb on + strand, within ulaR at 4415.879 kb on - strand, within ulaR at 4416.008 kb on + strand, within ulaR at 4416.037 kb on + strand, within ulaR at 4416.040 kb on - strand, within ulaR at 4416.050 kb on - strand, within ulaR at 4416.178 kb on - strand, within ulaR at 4416.291 kb on + strand, within ulaR at 4416.291 kb on + strand, within ulaR at 4416.291 kb on + strand, within ulaR at 4416.459 kb on + strand at 4416.555 kb on + strand at 4416.555 kb on + strand at 4416.568 kb on - strand at 4416.590 kb on - strand at 4416.639 kb on - strand at 4416.646 kb on + strand at 4416.653 kb on - strand at 4416.660 kb on + strand at 4416.660 kb on + strand at 4416.736 kb on - strand, within yjfR at 4416.758 kb on - strand, within yjfR at 4416.856 kb on + strand, within yjfR at 4416.956 kb on - strand, within yjfR at 4417.019 kb on - strand, within yjfR at 4417.079 kb on + strand, within yjfR at 4417.087 kb on + strand, within yjfR at 4417.095 kb on - strand, within yjfR at 4417.192 kb on - strand, within yjfR at 4417.192 kb on - strand, within yjfR at 4417.282 kb on + strand, within yjfR at 4417.312 kb on - strand, within yjfR at 4417.312 kb on - strand, within yjfR at 4417.392 kb on + strand, within yjfR at 4417.392 kb on + strand, within yjfR at 4417.407 kb on + strand, within yjfR at 4417.428 kb on + strand, within yjfR
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 4,414,722 - yjfO b4189 0.78 +0.5 4,414,746 - yjfO b4189 0.85 +0.2 4,414,791 - -0.0 4,414,791 - -0.5 4,414,795 + -0.0 4,414,800 - +0.4 4,414,800 - -0.3 4,414,825 - +0.0 4,414,838 - +0.3 4,414,838 - -0.4 4,414,885 + +0.0 4,414,899 + -0.3 4,414,899 + -0.1 4,415,030 - +1.5 4,415,030 - +1.3 4,415,050 - yjfP b4190 0.10 +1.2 4,415,058 - yjfP b4190 0.11 +0.6 4,415,058 - yjfP b4190 0.11 +1.4 4,415,058 - yjfP b4190 0.11 +1.3 4,415,086 - yjfP b4190 0.15 +0.9 4,415,103 - yjfP b4190 0.17 +0.5 4,415,171 + yjfP b4190 0.26 +0.9 4,415,171 + yjfP b4190 0.26 +0.2 4,415,370 + yjfP b4190 0.53 +1.1 4,415,370 + yjfP b4190 0.53 +1.1 4,415,399 + yjfP b4190 0.57 +1.3 4,415,399 + yjfP b4190 0.57 +0.8 4,415,526 - yjfP b4190 0.73 +1.5 4,415,562 - yjfP b4190 0.78 +1.2 4,415,590 - yjfP b4190 0.82 +0.7 4,415,620 + yjfP b4190 0.86 +0.5 4,415,620 + yjfP b4190 0.86 +1.0 4,415,632 + yjfP b4190 0.88 +1.0 4,415,632 + yjfP b4190 0.88 +1.3 4,415,657 - +0.9 4,415,724 - +0.4 4,415,724 - +0.0 4,415,767 - -1.1 4,415,767 - -0.2 4,415,820 + ulaR b4191 0.13 -3.0 4,415,879 + ulaR b4191 0.21 -0.6 4,415,879 + ulaR b4191 0.21 -1.8 4,415,879 - ulaR b4191 0.21 -1.9 4,416,008 + ulaR b4191 0.38 -2.8 4,416,037 + ulaR b4191 0.42 -0.3 4,416,040 - ulaR b4191 0.42 -2.2 4,416,050 - ulaR b4191 0.44 -0.3 4,416,178 - ulaR b4191 0.60 -2.4 4,416,291 + ulaR b4191 0.75 -0.7 4,416,291 + ulaR b4191 0.75 -2.1 4,416,291 + ulaR b4191 0.75 -0.9 4,416,459 + -1.2 4,416,555 + +0.1 4,416,555 + +0.1 4,416,568 - +0.1 4,416,590 - -0.3 4,416,639 - +0.4 4,416,646 + -0.2 4,416,653 - -0.9 4,416,660 + +0.0 4,416,660 + -0.2 4,416,736 - yjfR b4192 0.14 -0.1 4,416,758 - yjfR b4192 0.16 -0.1 4,416,856 + yjfR b4192 0.26 +0.1 4,416,956 - yjfR b4192 0.35 +0.1 4,417,019 - yjfR b4192 0.41 -0.2 4,417,079 + yjfR b4192 0.46 +0.0 4,417,087 + yjfR b4192 0.47 +0.0 4,417,095 - yjfR b4192 0.48 +0.1 4,417,192 - yjfR b4192 0.57 +0.4 4,417,192 - yjfR b4192 0.57 +0.3 4,417,282 + yjfR b4192 0.66 +0.2 4,417,312 - yjfR b4192 0.68 -0.4 4,417,312 - yjfR b4192 0.68 -0.3 4,417,392 + yjfR b4192 0.76 -0.7 4,417,392 + yjfR b4192 0.76 +0.2 4,417,407 + yjfR b4192 0.77 -0.3 4,417,428 + yjfR b4192 0.79 +0.1
Or see this region's nucleotide sequence