Strain Fitness in Escherichia coli BW25113 around b4099

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnK and phnJ overlap by 4 nucleotidesphnJ and phnI overlap by 8 nucleotidesphnI and phnH overlap by 1 nucleotidesphnH and phnG overlap by 4 nucleotides b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787 phnK b4098: phnJ - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,784 to 4,317,629 phnJ b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686 phnI b4100: phnH - carbon-phosphorus lyase complex subunit (NCBI), at 4,318,686 to 4,319,270 phnH b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719 phnG Position (kb) 4317 4318 4319Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4316.654 kb on - strand, within phnKat 4316.891 kb on - strand, within phnJat 4316.929 kb on + strand, within phnJat 4316.929 kb on + strand, within phnJat 4316.930 kb on - strand, within phnJat 4316.932 kb on + strand, within phnJat 4317.020 kb on + strand, within phnJat 4317.028 kb on - strand, within phnJat 4317.034 kb on - strand, within phnJat 4317.071 kb on + strand, within phnJat 4317.095 kb on + strand, within phnJat 4317.114 kb on + strand, within phnJat 4317.114 kb on + strand, within phnJat 4317.122 kb on - strandat 4317.154 kb on + strand, within phnJat 4317.159 kb on + strand, within phnJat 4317.168 kb on - strand, within phnJat 4317.177 kb on + strand, within phnJat 4317.177 kb on + strand, within phnJat 4317.210 kb on - strand, within phnJat 4317.215 kb on - strand, within phnJat 4317.303 kb on + strand, within phnJat 4317.340 kb on - strand, within phnJat 4317.349 kb on + strand, within phnJat 4317.394 kb on - strand, within phnJat 4317.402 kb on - strand, within phnJat 4317.428 kb on + strand, within phnJat 4317.428 kb on + strand, within phnJat 4317.481 kb on + strand, within phnJat 4317.481 kb on + strand, within phnJat 4317.620 kb on + strandat 4317.620 kb on + strandat 4317.631 kb on + strandat 4317.631 kb on + strandat 4317.634 kb on - strandat 4317.639 kb on - strandat 4317.658 kb on - strandat 4317.658 kb on - strandat 4317.686 kb on + strandat 4317.705 kb on + strandat 4317.708 kb on + strandat 4317.708 kb on + strandat 4317.853 kb on + strand, within phnIat 4317.854 kb on - strand, within phnIat 4317.980 kb on + strand, within phnIat 4318.053 kb on + strand, within phnIat 4318.053 kb on + strand, within phnIat 4318.081 kb on - strand, within phnIat 4318.081 kb on - strand, within phnIat 4318.138 kb on - strand, within phnIat 4318.217 kb on - strand, within phnIat 4318.321 kb on - strand, within phnIat 4318.326 kb on - strand, within phnIat 4318.392 kb on + strand, within phnIat 4318.400 kb on + strand, within phnIat 4318.489 kb on - strand, within phnIat 4318.549 kb on + strand, within phnIat 4318.635 kb on - strandat 4318.635 kb on - strandat 4318.687 kb on - strandat 4318.702 kb on - strandat 4318.748 kb on - strand, within phnHat 4318.799 kb on - strand, within phnHat 4318.814 kb on + strand, within phnHat 4318.855 kb on + strand, within phnHat 4318.855 kb on + strand, within phnHat 4319.165 kb on + strand, within phnHat 4319.223 kb on + strandat 4319.223 kb on + strandat 4319.223 kb on + strandat 4319.228 kb on + strandat 4319.258 kb on + strandat 4319.258 kb on + strandat 4319.259 kb on + strandat 4319.274 kb on - strandat 4319.279 kb on - strandat 4319.279 kb on - strandat 4319.354 kb on - strand, within phnGat 4319.366 kb on - strand, within phnGat 4319.379 kb on - strand, within phnGat 4319.434 kb on - strand, within phnGat 4319.446 kb on - strand, within phnGat 4319.573 kb on + strand, within phnG

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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4,316,654 - phnK b4097 0.82 -3.0
4,316,891 - phnJ b4098 0.13 -1.5
4,316,929 + phnJ b4098 0.17 -0.4
4,316,929 + phnJ b4098 0.17 -0.4
4,316,930 - phnJ b4098 0.17 -1.9
4,316,932 + phnJ b4098 0.17 +0.3
4,317,020 + phnJ b4098 0.28 -0.0
4,317,028 - phnJ b4098 0.29 -0.4
4,317,034 - phnJ b4098 0.30 -1.4
4,317,071 + phnJ b4098 0.34 -0.4
4,317,095 + phnJ b4098 0.37 -0.0
4,317,114 + phnJ b4098 0.39 +0.5
4,317,114 + phnJ b4098 0.39 -1.0
4,317,122 - +1.2
4,317,154 + phnJ b4098 0.44 -0.6
4,317,159 + phnJ b4098 0.44 -0.2
4,317,168 - phnJ b4098 0.45 -1.2
4,317,177 + phnJ b4098 0.46 +0.6
4,317,177 + phnJ b4098 0.46 +0.2
4,317,210 - phnJ b4098 0.50 -2.8
4,317,215 - phnJ b4098 0.51 -2.0
4,317,303 + phnJ b4098 0.61 -0.5
4,317,340 - phnJ b4098 0.66 -0.4
4,317,349 + phnJ b4098 0.67 -0.2
4,317,394 - phnJ b4098 0.72 -1.8
4,317,402 - phnJ b4098 0.73 -0.2
4,317,428 + phnJ b4098 0.76 -0.4
4,317,428 + phnJ b4098 0.76 -0.2
4,317,481 + phnJ b4098 0.82 -0.4
4,317,481 + phnJ b4098 0.82 -0.7
4,317,620 + +0.8
4,317,620 + -0.5
4,317,631 + -0.6
4,317,631 + -0.8
4,317,634 - -1.2
4,317,639 - -3.2
4,317,658 - -2.4
4,317,658 - -3.3
4,317,686 + -0.6
4,317,705 + +0.2
4,317,708 + +0.8
4,317,708 + +0.4
4,317,853 + phnI b4099 0.22 -0.2
4,317,854 - phnI b4099 0.22 +1.9
4,317,980 + phnI b4099 0.34 -0.2
4,318,053 + phnI b4099 0.40 +0.1
4,318,053 + phnI b4099 0.40 -0.1
4,318,081 - phnI b4099 0.43 -0.7
4,318,081 - phnI b4099 0.43 -1.5
4,318,138 - phnI b4099 0.48 -0.5
4,318,217 - phnI b4099 0.56 -0.2
4,318,321 - phnI b4099 0.66 -2.3
4,318,326 - phnI b4099 0.66 -0.6
4,318,392 + phnI b4099 0.72 -0.6
4,318,400 + phnI b4099 0.73 +0.0
4,318,489 - phnI b4099 0.81 -0.9
4,318,549 + phnI b4099 0.87 +0.2
4,318,635 - +0.3
4,318,635 - -0.3
4,318,687 - -1.1
4,318,702 - -2.1
4,318,748 - phnH b4100 0.11 -1.8
4,318,799 - phnH b4100 0.19 -1.3
4,318,814 + phnH b4100 0.22 -0.4
4,318,855 + phnH b4100 0.29 -0.6
4,318,855 + phnH b4100 0.29 -0.4
4,319,165 + phnH b4100 0.82 -0.0
4,319,223 + +0.2
4,319,223 + +0.1
4,319,223 + -0.7
4,319,228 + +0.1
4,319,258 + +0.1
4,319,258 + -0.0
4,319,259 + +0.0
4,319,274 - -1.1
4,319,279 - +0.0
4,319,279 - -1.0
4,319,354 - phnG b4101 0.19 -1.6
4,319,366 - phnG b4101 0.22 -2.3
4,319,379 - phnG b4101 0.25 -2.5
4,319,434 - phnG b4101 0.37 -0.2
4,319,446 - phnG b4101 0.40 -1.8
4,319,573 + phnG b4101 0.68 +0.2

Or see this region's nucleotide sequence