Strain Fitness in Escherichia coli BW25113 around b4095

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnP and phnO are separated by 1 nucleotidesphnO and phnN overlap by 14 nucleotidesphnN and phnM overlap by 1 nucleotidesphnM and phnL overlap by 4 nucleotidesphnL and phnK are separated by 110 nucleotides b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125 phnP b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561 phnO b4094: phnN - ribose 1,5-bisphosphokinase (NCBI), at 4,313,548 to 4,314,105 phnN b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241 phnM b4096: phnL - carbon-phosphorus lyase complex subunit (NCBI), at 4,315,238 to 4,315,918 phnL b4097: phnK - carbon-phosphorus lyase complex subunit (NCBI), at 4,316,029 to 4,316,787 phnK Position (kb) 4314 4315 4316Strain fitness (log2 ratio) -3 -2 -1 0 1at 4313.115 kb on - strandat 4313.115 kb on - strandat 4313.127 kb on + strandat 4313.185 kb on - strand, within phnOat 4313.195 kb on - strand, within phnOat 4313.274 kb on + strand, within phnOat 4313.433 kb on + strand, within phnOat 4313.502 kb on - strand, within phnOat 4313.502 kb on - strand, within phnOat 4313.529 kb on - strandat 4313.529 kb on - strandat 4313.565 kb on - strandat 4313.634 kb on + strand, within phnNat 4313.662 kb on - strand, within phnNat 4313.821 kb on + strand, within phnNat 4313.821 kb on + strand, within phnNat 4313.881 kb on - strand, within phnNat 4313.881 kb on - strand, within phnNat 4313.956 kb on - strand, within phnNat 4314.001 kb on - strand, within phnNat 4314.043 kb on + strand, within phnNat 4314.122 kb on - strandat 4314.213 kb on + strandat 4314.213 kb on + strandat 4314.357 kb on - strand, within phnMat 4314.363 kb on + strand, within phnMat 4314.396 kb on + strand, within phnMat 4314.445 kb on + strand, within phnMat 4314.445 kb on + strand, within phnMat 4314.742 kb on + strand, within phnMat 4314.819 kb on + strand, within phnMat 4314.819 kb on + strand, within phnMat 4314.822 kb on - strand, within phnMat 4314.822 kb on - strand, within phnMat 4314.857 kb on - strand, within phnMat 4314.857 kb on - strand, within phnMat 4315.006 kb on - strand, within phnMat 4315.181 kb on - strandat 4315.192 kb on - strandat 4315.192 kb on - strandat 4315.200 kb on - strandat 4315.288 kb on - strandat 4315.408 kb on + strand, within phnLat 4315.507 kb on + strand, within phnLat 4315.516 kb on - strand, within phnLat 4315.516 kb on - strand, within phnLat 4315.585 kb on - strand, within phnLat 4315.585 kb on - strand, within phnLat 4315.720 kb on - strand, within phnLat 4315.740 kb on + strand, within phnLat 4315.740 kb on + strand, within phnLat 4315.751 kb on + strand, within phnLat 4316.095 kb on + strandat 4316.185 kb on + strand, within phnKat 4316.185 kb on + strand, within phnKat 4316.208 kb on - strand, within phnKat 4316.211 kb on + strand, within phnK

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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4,313,115 - +0.4
4,313,115 - +0.4
4,313,127 + +0.3
4,313,185 - phnO b4093 0.13 -0.1
4,313,195 - phnO b4093 0.16 -0.0
4,313,274 + phnO b4093 0.34 +0.4
4,313,433 + phnO b4093 0.70 -0.2
4,313,502 - phnO b4093 0.86 -0.8
4,313,502 - phnO b4093 0.86 +0.3
4,313,529 - +0.6
4,313,529 - +0.8
4,313,565 - -0.3
4,313,634 + phnN b4094 0.15 +0.9
4,313,662 - phnN b4094 0.20 -0.8
4,313,821 + phnN b4094 0.49 -0.4
4,313,821 + phnN b4094 0.49 -0.1
4,313,881 - phnN b4094 0.60 -0.1
4,313,881 - phnN b4094 0.60 +0.7
4,313,956 - phnN b4094 0.73 -0.1
4,314,001 - phnN b4094 0.81 +0.8
4,314,043 + phnN b4094 0.89 +0.2
4,314,122 - -3.5
4,314,213 + -0.4
4,314,213 + -0.3
4,314,357 - phnM b4095 0.22 -1.2
4,314,363 + phnM b4095 0.23 -1.4
4,314,396 + phnM b4095 0.26 +0.1
4,314,445 + phnM b4095 0.30 +0.2
4,314,445 + phnM b4095 0.30 +0.1
4,314,742 + phnM b4095 0.56 -0.3
4,314,819 + phnM b4095 0.63 +1.2
4,314,819 + phnM b4095 0.63 -1.0
4,314,822 - phnM b4095 0.63 +0.2
4,314,822 - phnM b4095 0.63 -0.6
4,314,857 - phnM b4095 0.66 -0.8
4,314,857 - phnM b4095 0.66 -0.5
4,315,006 - phnM b4095 0.79 +0.3
4,315,181 - -0.7
4,315,192 - -1.2
4,315,192 - -0.1
4,315,200 - -1.5
4,315,288 - -2.0
4,315,408 + phnL b4096 0.25 +0.8
4,315,507 + phnL b4096 0.40 -1.2
4,315,516 - phnL b4096 0.41 -0.3
4,315,516 - phnL b4096 0.41 -0.9
4,315,585 - phnL b4096 0.51 -0.1
4,315,585 - phnL b4096 0.51 +0.8
4,315,720 - phnL b4096 0.71 -1.2
4,315,740 + phnL b4096 0.74 -0.8
4,315,740 + phnL b4096 0.74 -0.6
4,315,751 + phnL b4096 0.75 -0.1
4,316,095 + -0.4
4,316,185 + phnK b4097 0.21 -1.0
4,316,185 + phnK b4097 0.21 +0.8
4,316,208 - phnK b4097 0.24 -2.4
4,316,211 + phnK b4097 0.24 -2.4

Or see this region's nucleotide sequence