Strain Fitness in Escherichia coli BW25113 around b4093

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjdP and phnP are separated by 146 nucleotidesphnP and phnO are separated by 1 nucleotidesphnO and phnN overlap by 14 nucleotidesphnN and phnM overlap by 1 nucleotides b4487: yjdP - hypothetical protein (NCBI), at 4,311,891 to 4,312,220 yjdP b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125 phnP b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561 phnO b4094: phnN - ribose 1,5-bisphosphokinase (NCBI), at 4,313,548 to 4,314,105 phnN b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241 phnM Position (kb) 4313 4314Strain fitness (log2 ratio) -3 -2 -1 0 1at 4312.193 kb on + strandat 4312.202 kb on + strandat 4312.227 kb on - strandat 4312.256 kb on + strandat 4312.263 kb on - strandat 4312.338 kb on - strandat 4312.375 kb on + strandat 4312.418 kb on - strandat 4312.418 kb on - strandat 4312.567 kb on + strand, within phnPat 4312.658 kb on - strand, within phnPat 4312.658 kb on - strand, within phnPat 4312.663 kb on - strand, within phnPat 4312.663 kb on - strand, within phnPat 4312.668 kb on - strand, within phnPat 4312.668 kb on - strand, within phnPat 4312.700 kb on + strand, within phnPat 4312.714 kb on - strand, within phnPat 4312.720 kb on + strand, within phnPat 4312.720 kb on + strand, within phnPat 4312.721 kb on - strand, within phnPat 4312.728 kb on - strand, within phnPat 4312.788 kb on - strand, within phnPat 4312.803 kb on + strand, within phnPat 4312.808 kb on + strand, within phnPat 4312.948 kb on - strand, within phnPat 4312.965 kb on - strand, within phnPat 4313.024 kb on - strand, within phnPat 4313.041 kb on + strand, within phnPat 4313.060 kb on + strandat 4313.094 kb on - strandat 4313.115 kb on - strandat 4313.115 kb on - strandat 4313.127 kb on + strandat 4313.185 kb on - strand, within phnOat 4313.195 kb on - strand, within phnOat 4313.274 kb on + strand, within phnOat 4313.433 kb on + strand, within phnOat 4313.502 kb on - strand, within phnOat 4313.502 kb on - strand, within phnOat 4313.529 kb on - strandat 4313.529 kb on - strandat 4313.565 kb on - strandat 4313.634 kb on + strand, within phnNat 4313.662 kb on - strand, within phnNat 4313.821 kb on + strand, within phnNat 4313.821 kb on + strand, within phnNat 4313.881 kb on - strand, within phnNat 4313.881 kb on - strand, within phnNat 4313.956 kb on - strand, within phnNat 4314.001 kb on - strand, within phnNat 4314.043 kb on + strand, within phnNat 4314.122 kb on - strandat 4314.213 kb on + strandat 4314.213 kb on + strandat 4314.357 kb on - strand, within phnMat 4314.363 kb on + strand, within phnMat 4314.396 kb on + strand, within phnMat 4314.445 kb on + strand, within phnMat 4314.445 kb on + strand, within phnM

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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4,312,193 + +0.3
4,312,202 + +0.2
4,312,227 - -0.2
4,312,256 + -0.0
4,312,263 - -0.1
4,312,338 - +0.4
4,312,375 + -0.5
4,312,418 - +0.4
4,312,418 - -0.4
4,312,567 + phnP b4092 0.26 +1.6
4,312,658 - phnP b4092 0.38 +0.3
4,312,658 - phnP b4092 0.38 -0.3
4,312,663 - phnP b4092 0.39 -0.3
4,312,663 - phnP b4092 0.39 +0.6
4,312,668 - phnP b4092 0.40 -0.5
4,312,668 - phnP b4092 0.40 +0.3
4,312,700 + phnP b4092 0.44 -0.0
4,312,714 - phnP b4092 0.46 +0.1
4,312,720 + phnP b4092 0.47 +0.2
4,312,720 + phnP b4092 0.47 -1.2
4,312,721 - phnP b4092 0.47 -0.1
4,312,728 - phnP b4092 0.48 +0.2
4,312,788 - phnP b4092 0.55 +0.1
4,312,803 + phnP b4092 0.57 -0.4
4,312,808 + phnP b4092 0.58 +0.4
4,312,948 - phnP b4092 0.77 +0.7
4,312,965 - phnP b4092 0.79 +0.1
4,313,024 - phnP b4092 0.87 +0.2
4,313,041 + phnP b4092 0.89 -0.7
4,313,060 + -0.1
4,313,094 - +0.5
4,313,115 - +0.4
4,313,115 - +0.4
4,313,127 + +0.3
4,313,185 - phnO b4093 0.13 -0.1
4,313,195 - phnO b4093 0.16 -0.0
4,313,274 + phnO b4093 0.34 +0.4
4,313,433 + phnO b4093 0.70 -0.2
4,313,502 - phnO b4093 0.86 -0.8
4,313,502 - phnO b4093 0.86 +0.3
4,313,529 - +0.6
4,313,529 - +0.8
4,313,565 - -0.3
4,313,634 + phnN b4094 0.15 +0.9
4,313,662 - phnN b4094 0.20 -0.8
4,313,821 + phnN b4094 0.49 -0.4
4,313,821 + phnN b4094 0.49 -0.1
4,313,881 - phnN b4094 0.60 -0.1
4,313,881 - phnN b4094 0.60 +0.7
4,313,956 - phnN b4094 0.73 -0.1
4,314,001 - phnN b4094 0.81 +0.8
4,314,043 + phnN b4094 0.89 +0.2
4,314,122 - -3.5
4,314,213 + -0.4
4,314,213 + -0.3
4,314,357 - phnM b4095 0.22 -1.2
4,314,363 + phnM b4095 0.23 -1.4
4,314,396 + phnM b4095 0.26 +0.1
4,314,445 + phnM b4095 0.30 +0.2
4,314,445 + phnM b4095 0.30 +0.1

Or see this region's nucleotide sequence