Strain Fitness in Escherichia coli BW25113 around b3946

Experiment: Ying_Nucleobase14 rep B; time point 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_Nucleobase14 rep B; time point 2
4,136,159	+	gldA	b3945	0.18	-0.8
4,136,201	+	gldA	b3945	0.22	+0.1
4,136,229	-	gldA	b3945	0.25	-0.3
4,136,229	-	gldA	b3945	0.25	+0.5
4,136,248	+	gldA	b3945	0.27	-0.1
4,136,248	+	gldA	b3945	0.27	+0.2
4,136,260	-	gldA	b3945	0.28	+0.9
4,136,260	-	gldA	b3945	0.28	+0.6
4,136,277	-	gldA	b3945	0.29	-1.0
4,136,353	+	gldA	b3945	0.36	+0.1
4,136,361	-	gldA	b3945	0.37	-0.1
4,136,390	-	gldA	b3945	0.39	-0.2
4,136,390	-	gldA	b3945	0.39	-0.2
4,136,435	+	gldA	b3945	0.43	-0.2
4,136,444	-	gldA	b3945	0.44	-0.3
4,136,454	-	gldA	b3945	0.45	-0.6
4,136,474	-	gldA	b3945	0.47	-0.1
4,136,474	-	gldA	b3945	0.47	-0.1
4,136,474	-	gldA	b3945	0.47	+0.4
4,136,474	-	gldA	b3945	0.47	+0.7
4,136,508	+	gldA	b3945	0.50	+0.3
4,136,557	-	gldA	b3945	0.55	+0.0
4,136,562	+	gldA	b3945	0.55	+0.0
4,136,638	+	gldA	b3945	0.62	+0.4
4,136,667	-	gldA	b3945	0.64	-0.1
4,136,670	+	gldA	b3945	0.65	-0.3
4,136,672	-	gldA	b3945	0.65	+0.1
4,136,690	-	gldA	b3945	0.67	-0.2
4,136,725	+	gldA	b3945	0.70	-0.2
4,136,733	-	gldA	b3945	0.70	+0.1
4,136,733	-	gldA	b3945	0.70	-0.4
4,136,733	-	gldA	b3945	0.70	+0.1
4,136,738	-	gldA	b3945	0.71	+0.1
4,136,738	-	gldA	b3945	0.71	-0.1
4,136,784	+	gldA	b3945	0.75	-0.4
4,136,784	+	gldA	b3945	0.75	-0.9
4,136,812	-	gldA	b3945	0.78	-0.0
4,136,812	-	gldA	b3945	0.78	+0.6
4,136,916	+				+0.8
4,136,970	+				-1.4
4,136,970	+				-0.3
4,137,030	-				-0.0
4,137,061	-				+0.1
4,137,061	-				-0.1
4,137,105	-				+0.3
4,137,105	-				-0.7
4,137,105	-				+0.2
4,137,196	+	fsaB	b3946	0.19	-0.1
4,137,214	+	fsaB	b3946	0.22	+0.2
4,137,215	-	fsaB	b3946	0.22	+0.1
4,137,234	-	fsaB	b3946	0.25	-0.4
4,137,288	+	fsaB	b3946	0.33	+0.7
4,137,288	+	fsaB	b3946	0.33	+0.3
4,137,288	+	fsaB	b3946	0.33	-0.2
4,137,291	-	fsaB	b3946	0.33	-1.7
4,137,296	-	fsaB	b3946	0.34	+0.5
4,137,296	-	fsaB	b3946	0.34	-0.3
4,137,344	-	fsaB	b3946	0.41	+0.2
4,137,389	-	fsaB	b3946	0.48	+0.7
4,137,389	-	fsaB	b3946	0.48	-1.0
4,137,393	+	fsaB	b3946	0.49	-0.4
4,137,396	+	fsaB	b3946	0.49	+0.2
4,137,401	-	fsaB	b3946	0.50	-0.5
4,137,401	-	fsaB	b3946	0.50	+0.9
4,137,404	-	fsaB	b3946	0.51	-0.4
4,137,404	-	fsaB	b3946	0.51	+0.3
4,137,404	-	fsaB	b3946	0.51	-0.9
4,137,404	-	fsaB	b3946	0.51	+0.0
4,137,468	-	fsaB	b3946	0.60	-0.1
4,137,507	+	fsaB	b3946	0.66	+0.5
4,137,525	-	fsaB	b3946	0.69	-0.2
4,137,525	-	fsaB	b3946	0.69	-0.9
4,137,539	-	fsaB	b3946	0.71	+0.1
4,137,539	-	fsaB	b3946	0.71	+0.1
4,137,552	+	fsaB	b3946	0.73	+0.1
4,137,610	+	fsaB	b3946	0.82	+0.1
4,137,647	+	fsaB	b3946	0.87	-0.4
4,137,703	+				-0.0
4,137,703	-				+0.2
4,137,705	-				-0.5
4,137,866	-				-0.8
4,137,866	-				+0.5
4,137,900	-				-0.5
4,137,900	-				-0.9
4,137,900	-				-0.7
4,138,103	-	ptsA	b3947	0.14	-1.3
4,138,103	-	ptsA	b3947	0.14	+0.1
4,138,112	-	ptsA	b3947	0.15	-0.4
4,138,124	-	ptsA	b3947	0.15	-0.0
4,138,205	-	ptsA	b3947	0.18	-0.8
4,138,205	-	ptsA	b3947	0.18	-0.5
4,138,208	-	ptsA	b3947	0.19	-0.2
4,138,259	+	ptsA	b3947	0.21	-0.7
4,138,351	+	ptsA	b3947	0.24	-0.4
4,138,396	+	ptsA	b3947	0.26	+0.4
4,138,398	+	ptsA	b3947	0.26	-0.9
4,138,398	+	ptsA	b3947	0.26	+0.4
4,138,444	-	ptsA	b3947	0.28	-1.1
4,138,444	-	ptsA	b3947	0.28	-0.2
4,138,446	+	ptsA	b3947	0.28	+0.1
4,138,459	+	ptsA	b3947	0.29	+0.2
4,138,467	-	ptsA	b3947	0.29	-0.2
4,138,662	-	ptsA	b3947	0.37	-0.1
4,138,705	+	ptsA	b3947	0.38	+0.6
4,138,705	+	ptsA	b3947	0.38	-0.4