Strain Fitness in Escherichia coli BW25113 around b3811

Experiment: Ying_Nucleobase14 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdapF and yigA overlap by 4 nucleotidesyigA and xerC overlap by 4 nucleotidesxerC and yigB overlap by 1 nucleotidesyigB and uvrD are separated by 83 nucleotides b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609 dapF b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313 yigA b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206 xerC b3812: yigB - predicted hydrolase (NCBI), at 3,995,206 to 3,995,922 yigB b3813: uvrD - DNA-dependent ATPase I and helicase II (NCBI), at 3,996,006 to 3,998,168 uvrD Position (kb) 3994 3995 3996Strain fitness (log2 ratio) -2 -1 0 1 2at 3993.601 kb on + strandat 3993.601 kb on + strandat 3993.637 kb on + strandat 3993.647 kb on - strandat 3993.778 kb on - strand, within yigAat 3993.778 kb on - strand, within yigAat 3993.782 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.813 kb on - strand, within yigAat 3993.815 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.827 kb on + strand, within yigAat 3993.829 kb on + strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.837 kb on - strand, within yigAat 3993.854 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.892 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.948 kb on - strand, within yigAat 3993.972 kb on - strand, within yigAat 3993.978 kb on - strand, within yigAat 3994.051 kb on - strand, within yigAat 3994.088 kb on - strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.090 kb on + strand, within yigAat 3994.093 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.098 kb on - strand, within yigAat 3994.170 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.192 kb on + strand, within yigAat 3994.202 kb on + strand, within yigAat 3994.210 kb on - strand, within yigAat 3994.279 kb on - strandat 3994.300 kb on - strandat 3994.300 kb on - strandat 3994.314 kb on - strandat 3994.318 kb on + strandat 3994.399 kb on + strandat 3994.765 kb on + strand, within xerCat 3994.776 kb on + strand, within xerCat 3994.932 kb on + strand, within xerCat 3995.087 kb on + strand, within xerCat 3995.093 kb on - strand, within xerCat 3995.093 kb on - strand, within xerCat 3995.095 kb on - strand, within xerCat 3995.113 kb on + strand, within xerCat 3995.165 kb on + strandat 3995.165 kb on + strandat 3995.246 kb on + strandat 3995.246 kb on + strandat 3995.251 kb on + strandat 3995.308 kb on + strand, within yigBat 3995.308 kb on + strand, within yigBat 3995.316 kb on - strand, within yigBat 3995.338 kb on + strand, within yigBat 3995.341 kb on + strand, within yigBat 3995.351 kb on - strand, within yigBat 3995.379 kb on - strand, within yigBat 3995.391 kb on - strand, within yigBat 3995.400 kb on + strand, within yigBat 3995.567 kb on + strand, within yigBat 3995.615 kb on + strand, within yigBat 3995.615 kb on + strand, within yigBat 3995.782 kb on + strand, within yigBat 3995.826 kb on - strand, within yigBat 3995.864 kb on + strandat 3995.875 kb on - strandat 3995.875 kb on - strandat 3995.950 kb on - strandat 3995.962 kb on + strandat 3996.013 kb on + strandat 3996.013 kb on + strandat 3996.013 kb on + strandat 3996.013 kb on + strandat 3996.013 kb on + strandat 3996.021 kb on - strandat 3996.037 kb on + strandat 3996.069 kb on + strandat 3996.095 kb on + strandat 3996.103 kb on + strandat 3996.111 kb on - strandat 3996.148 kb on + strandat 3996.150 kb on + strandat 3996.180 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2
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3,993,601 + +1.0
3,993,601 + -0.1
3,993,637 + -1.2
3,993,647 - -2.1
3,993,778 - yigA b3810 0.24 +0.1
3,993,778 - yigA b3810 0.24 +0.6
3,993,782 - yigA b3810 0.25 +0.5
3,993,813 - yigA b3810 0.29 +0.2
3,993,813 - yigA b3810 0.29 -0.8
3,993,815 + yigA b3810 0.30 +0.1
3,993,827 + yigA b3810 0.31 -0.2
3,993,827 + yigA b3810 0.31 -0.3
3,993,829 + yigA b3810 0.31 -0.3
3,993,837 - yigA b3810 0.33 -0.6
3,993,837 - yigA b3810 0.33 +0.2
3,993,854 - yigA b3810 0.35 -0.5
3,993,892 - yigA b3810 0.40 -0.5
3,993,892 - yigA b3810 0.40 +0.3
3,993,948 - yigA b3810 0.48 -0.5
3,993,948 - yigA b3810 0.48 +0.2
3,993,972 - yigA b3810 0.52 -0.3
3,993,978 - yigA b3810 0.53 +0.0
3,994,051 - yigA b3810 0.63 -0.5
3,994,088 - yigA b3810 0.68 -0.2
3,994,090 + yigA b3810 0.68 +0.8
3,994,090 + yigA b3810 0.68 -0.1
3,994,093 - yigA b3810 0.69 -0.9
3,994,098 - yigA b3810 0.69 -1.0
3,994,098 - yigA b3810 0.69 -0.4
3,994,098 - yigA b3810 0.69 -0.7
3,994,098 - yigA b3810 0.69 -1.3
3,994,098 - yigA b3810 0.69 -0.5
3,994,098 - yigA b3810 0.69 -0.2
3,994,170 + yigA b3810 0.80 -1.9
3,994,192 + yigA b3810 0.83 -0.7
3,994,192 + yigA b3810 0.83 -0.4
3,994,202 + yigA b3810 0.84 -0.0
3,994,210 - yigA b3810 0.85 -0.4
3,994,279 - -0.1
3,994,300 - -0.0
3,994,300 - -1.4
3,994,314 - -0.2
3,994,318 + -0.2
3,994,399 + -0.4
3,994,765 + xerC b3811 0.51 -0.0
3,994,776 + xerC b3811 0.52 +0.9
3,994,932 + xerC b3811 0.69 +0.6
3,995,087 + xerC b3811 0.87 -1.4
3,995,093 - xerC b3811 0.87 +0.9
3,995,093 - xerC b3811 0.87 -1.5
3,995,095 - xerC b3811 0.88 +2.5
3,995,113 + xerC b3811 0.90 +0.1
3,995,165 + -0.9
3,995,165 + +0.1
3,995,246 + +0.3
3,995,246 + -0.2
3,995,251 + -0.2
3,995,308 + yigB b3812 0.14 +0.4
3,995,308 + yigB b3812 0.14 -0.1
3,995,316 - yigB b3812 0.15 -0.2
3,995,338 + yigB b3812 0.18 -0.1
3,995,341 + yigB b3812 0.19 -0.3
3,995,351 - yigB b3812 0.20 +0.5
3,995,379 - yigB b3812 0.24 -0.6
3,995,391 - yigB b3812 0.26 -0.5
3,995,400 + yigB b3812 0.27 -0.9
3,995,567 + yigB b3812 0.50 -0.2
3,995,615 + yigB b3812 0.57 +0.5
3,995,615 + yigB b3812 0.57 -0.7
3,995,782 + yigB b3812 0.80 +0.4
3,995,826 - yigB b3812 0.86 -0.1
3,995,864 + +0.1
3,995,875 - -0.1
3,995,875 - +0.1
3,995,950 - +0.4
3,995,962 + +0.0
3,996,013 + +0.2
3,996,013 + +0.5
3,996,013 + +0.1
3,996,013 + +0.7
3,996,013 + +0.1
3,996,021 - -0.2
3,996,037 + -0.3
3,996,069 + +0.4
3,996,095 + +0.2
3,996,103 + +0.9
3,996,111 - +1.0
3,996,148 + -0.1
3,996,150 + +0.3
3,996,180 - -0.3

Or see this region's nucleotide sequence