Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggT and yggU overlap by 4 nucleotides yggU and yggV are separated by 7 nucleotides yggV and yggW overlap by 8 nucleotides yggW and yggM are separated by 154 nucleotides
b2952: yggT - predicted inner membrane protein (NCBI), at 3,093,842 to 3,094,408
yggT
b2953: yggU - orf, hypothetical protein (VIMSS), at 3,094,405 to 3,094,695
yggU
b2954: yggV - putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI), at 3,094,703 to 3,095,296
yggV
b2955: yggW - coproporphyrinogen III oxidase (NCBI), at 3,095,289 to 3,096,425
yggW
b2956: yggM - hypothetical protein (NCBI), at 3,096,580 to 3,097,587
yggM
Position (kb)
3095
3096
3097 Strain fitness (log2 ratio)
-2
-1
0
1 at 3094.290 kb on + strand, within yggT at 3094.319 kb on + strand, within yggT at 3094.324 kb on + strand, within yggT at 3094.333 kb on + strand, within yggT at 3094.333 kb on + strand, within yggT at 3094.391 kb on - strand at 3094.400 kb on + strand at 3094.400 kb on + strand at 3094.451 kb on + strand, within yggU at 3094.451 kb on + strand, within yggU at 3094.542 kb on + strand, within yggU at 3094.775 kb on + strand, within yggV at 3094.879 kb on + strand, within yggV at 3094.920 kb on - strand, within yggV at 3094.948 kb on + strand, within yggV at 3095.006 kb on + strand, within yggV at 3095.095 kb on - strand, within yggV at 3095.115 kb on + strand, within yggV at 3095.136 kb on + strand, within yggV at 3095.299 kb on - strand at 3095.380 kb on + strand at 3095.409 kb on - strand, within yggW at 3095.442 kb on - strand, within yggW at 3095.448 kb on + strand, within yggW at 3095.456 kb on - strand, within yggW at 3095.485 kb on + strand, within yggW at 3095.609 kb on - strand, within yggW at 3095.661 kb on - strand, within yggW at 3095.757 kb on - strand, within yggW at 3095.844 kb on - strand, within yggW at 3095.886 kb on + strand, within yggW at 3095.902 kb on + strand, within yggW at 3096.070 kb on - strand, within yggW at 3096.104 kb on - strand, within yggW at 3096.146 kb on + strand, within yggW at 3096.179 kb on - strand, within yggW at 3096.242 kb on + strand, within yggW at 3096.242 kb on + strand, within yggW at 3096.281 kb on - strand, within yggW at 3096.319 kb on - strand at 3096.319 kb on - strand at 3096.327 kb on + strand at 3096.453 kb on + strand at 3096.550 kb on - strand at 3096.579 kb on - strand at 3096.846 kb on + strand, within yggM at 3096.847 kb on - strand, within yggM at 3096.858 kb on + strand, within yggM at 3096.866 kb on - strand, within yggM at 3096.866 kb on - strand, within yggM at 3096.899 kb on - strand, within yggM at 3097.084 kb on - strand, within yggM at 3097.107 kb on - strand, within yggM at 3097.207 kb on - strand, within yggM at 3097.252 kb on + strand, within yggM at 3097.252 kb on + strand, within yggM at 3097.420 kb on - strand, within yggM at 3097.420 kb on - strand, within yggM
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 3,094,290 + yggT b2952 0.79 -0.5 3,094,319 + yggT b2952 0.84 -0.1 3,094,324 + yggT b2952 0.85 -0.8 3,094,333 + yggT b2952 0.87 -0.1 3,094,333 + yggT b2952 0.87 -0.3 3,094,391 - -0.3 3,094,400 + +0.1 3,094,400 + +0.4 3,094,451 + yggU b2953 0.16 -0.5 3,094,451 + yggU b2953 0.16 +0.2 3,094,542 + yggU b2953 0.47 -0.1 3,094,775 + yggV b2954 0.12 +0.2 3,094,879 + yggV b2954 0.30 -1.8 3,094,920 - yggV b2954 0.37 +0.0 3,094,948 + yggV b2954 0.41 +0.1 3,095,006 + yggV b2954 0.51 -0.1 3,095,095 - yggV b2954 0.66 -0.7 3,095,115 + yggV b2954 0.69 -0.5 3,095,136 + yggV b2954 0.73 -0.8 3,095,299 - -0.2 3,095,380 + -0.4 3,095,409 - yggW b2955 0.11 -0.3 3,095,442 - yggW b2955 0.13 -0.7 3,095,448 + yggW b2955 0.14 -0.8 3,095,456 - yggW b2955 0.15 -0.8 3,095,485 + yggW b2955 0.17 -1.1 3,095,609 - yggW b2955 0.28 -0.6 3,095,661 - yggW b2955 0.33 -0.2 3,095,757 - yggW b2955 0.41 -0.1 3,095,844 - yggW b2955 0.49 +0.3 3,095,886 + yggW b2955 0.53 -0.6 3,095,902 + yggW b2955 0.54 -1.9 3,096,070 - yggW b2955 0.69 -0.9 3,096,104 - yggW b2955 0.72 +0.1 3,096,146 + yggW b2955 0.75 -0.1 3,096,179 - yggW b2955 0.78 -0.8 3,096,242 + yggW b2955 0.84 -0.3 3,096,242 + yggW b2955 0.84 -1.2 3,096,281 - yggW b2955 0.87 -1.4 3,096,319 - -1.4 3,096,319 - -0.7 3,096,327 + -0.8 3,096,453 + +0.1 3,096,550 - +0.0 3,096,579 - +0.3 3,096,846 + yggM b2956 0.26 -0.4 3,096,847 - yggM b2956 0.26 -0.0 3,096,858 + yggM b2956 0.28 +0.3 3,096,866 - yggM b2956 0.28 +0.3 3,096,866 - yggM b2956 0.28 +0.5 3,096,899 - yggM b2956 0.32 -0.0 3,097,084 - yggM b2956 0.50 -0.1 3,097,107 - yggM b2956 0.52 +1.7 3,097,207 - yggM b2956 0.62 +0.1 3,097,252 + yggM b2956 0.67 +0.2 3,097,252 + yggM b2956 0.67 +0.8 3,097,420 - yggM b2956 0.83 +0.4 3,097,420 - yggM b2956 0.83 +0.4
Or see this region's nucleotide sequence